In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts

Mustafa ERGÜL, (Sivas Cumhuriyet Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Sivas, Türkiye)
Merve ERGÜL, (Sivas Cumhuriyet Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, Sivas, Türkiye)
Nuraniye ERUYGUR, (Selçuk Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Konya, Türkiye)
Mehmet ATAŞ, (Sivas Cumhuriyet Üniversitesi, Eczacılık Fakültesi, Mikrobiyoloji Anabilim Dalı, Sivas, Türkiye)
Esra UÇAR (Sivas Cumhuriyet Üniversitesi, Sivas Meslek Yüksekokulu, Tıbbi ve Aromatik Bitkiler Bölümü, Sivas, Türkiye)
Turkish Journal of Pharmaceutical Sciences
12 3
Yıl: 2019 Cilt: 16 Sayı: 4 Sayfa Aralığı: 401 - 409 Metin Dili: İngilizce DOI: 10.4274/tjps.galenos.2018.70037 İndeks Tarihi: 26-08-2020

In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts

Öz:
Objectives: The present study aimed to investigate the inhibitory activities of enzymes related to diabetes mellitus and Alzheimer’s disease of themethanol and water extracts of Ficus carica leaf extracts. The bioactive compounds and anticancer, antioxidant, and antimicrobial effects of theextracts were also investigated.Materials and Methods: The bioactive compounds in the extracts were determined by gas chromatography-mass spectrometry. The antioxidantactivity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6 sulphonic acid) (ABTS) radical scavenging,total phenol and flavonoid content, ferric reducing power, and iron chelating method. The anticancer, anticholinesterase, and antimicrobial effectswere investigated using the XTT assay, Ellman method, and microdilution, respectively.Results: Our results showed that between the water and methanol extracts there was a difference in terms of chemical composition. The antioxidantresults suggested that both extracts have strong antioxidant activity. Similarly, both extracts showed strong α-glucosidase and α-amylase inhibitionactivity, while the water extract had higher inhibition activity than the methanol extract against acetylcholinesterase and butyrylcholinesterase. Themethanol extract of F. carica exhibited significant anticancer activity on MDA-MB-231 cells and showed moderate antimicrobial activities againstEscherichia coli and Staphylococcus aureus.Conclusion: Our results suggest that F. carica leaves could be a valuable source for developing a promising therapeutic agent in cancer, diabetes,and Alzheimer’s disease.
Anahtar Kelime:

Ficus carica Yaprak Ekstrelerinin Kimyasal Bileşiminin ve Çeşitli Biyolojik Aktivitelerinin İn Vitro Değerlendirilmesi

Öz:
Amaç: Bu çalışmada, Ficus carica yapraklarına ait su ve metanol ekstrelerinin diyabet ve Alzheimer hastalığı ile ilişkili enzimlerin inhibisyonu üzerine etkisinin araştırılması amaçlanmıştır. Ayrıca, ekstrelere ait biyoaktif bileşenler, antikanser, antioksidan ve antimikrobiyal etkiler de araştırılmıştır. Gereç ve Yöntemler: Ekstrelerdeki biyoaktif bileşikler gaz kromatografisi-kütle spektrometresi metodu ile belirlenmiştir. Antioksidan aktivite, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6 sulphonic acid) (ABTS) radikal süpürücü, toplam fenol ve flavonoid içeriği, ferrik indirgeme gücü ve demir şelasyon yöntemleriyle değerlendirilmiştir. Antikanser, antikolinesteraz ve antimikrobiyal etkinlikler ise sırasıyla XTT yöntemi, Ellman yöntemi ve mikrodilüsyon tekniği yöntemi ile belirlenmiştir. Bulgular: Elde ettiğimiz sonuçlar su ve metanol ekstreleri arasında kimyasal bileşim açısından farklılık olduğunu ve her iki ekstrenin de güçlü antioksidan aktiviteye sahip olduğunu göstermiştir. Benzer şekilde, her iki ekstrede güçlü α-glikozidaz ve α-amilaz aktivite gösterirken, su ekstresi metanole göre daha güçlü asetilkolinesteraz ve butirilkolinesteraz inhibisyon etkiye sahiptir. F. carica metanol ekstresi MDA-MB-231 hücreleri üzerinde güçlü antikanser etki, Escherichia coli ve Staphylococcus aureus’e karşı ise orta düzeyde antimikrobiyal etki göstermiştir. Sonuç: Bulgularımız, F. carica yapraklarının kanser, diyabet ve Alzheimer hastalığında umut verici bir terapötik ajan geliştirmek için değerli bir kaynak olabileceğini düşündürmektedir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ERGÜL M, ERGÜL M, ERUYGUR N, ATAŞ M, UÇAR E (2019). In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. , 401 - 409. 10.4274/tjps.galenos.2018.70037
Chicago ERGÜL Mustafa,ERGÜL Merve,ERUYGUR Nuraniye,ATAŞ Mehmet,UÇAR Esra In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. (2019): 401 - 409. 10.4274/tjps.galenos.2018.70037
MLA ERGÜL Mustafa,ERGÜL Merve,ERUYGUR Nuraniye,ATAŞ Mehmet,UÇAR Esra In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. , 2019, ss.401 - 409. 10.4274/tjps.galenos.2018.70037
AMA ERGÜL M,ERGÜL M,ERUYGUR N,ATAŞ M,UÇAR E In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. . 2019; 401 - 409. 10.4274/tjps.galenos.2018.70037
Vancouver ERGÜL M,ERGÜL M,ERUYGUR N,ATAŞ M,UÇAR E In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. . 2019; 401 - 409. 10.4274/tjps.galenos.2018.70037
IEEE ERGÜL M,ERGÜL M,ERUYGUR N,ATAŞ M,UÇAR E "In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts." , ss.401 - 409, 2019. 10.4274/tjps.galenos.2018.70037
ISNAD ERGÜL, Mustafa vd. "In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts". (2019), 401-409. https://doi.org/10.4274/tjps.galenos.2018.70037
APA ERGÜL M, ERGÜL M, ERUYGUR N, ATAŞ M, UÇAR E (2019). In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. Turkish Journal of Pharmaceutical Sciences, 16(4), 401 - 409. 10.4274/tjps.galenos.2018.70037
Chicago ERGÜL Mustafa,ERGÜL Merve,ERUYGUR Nuraniye,ATAŞ Mehmet,UÇAR Esra In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. Turkish Journal of Pharmaceutical Sciences 16, no.4 (2019): 401 - 409. 10.4274/tjps.galenos.2018.70037
MLA ERGÜL Mustafa,ERGÜL Merve,ERUYGUR Nuraniye,ATAŞ Mehmet,UÇAR Esra In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. Turkish Journal of Pharmaceutical Sciences, vol.16, no.4, 2019, ss.401 - 409. 10.4274/tjps.galenos.2018.70037
AMA ERGÜL M,ERGÜL M,ERUYGUR N,ATAŞ M,UÇAR E In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. Turkish Journal of Pharmaceutical Sciences. 2019; 16(4): 401 - 409. 10.4274/tjps.galenos.2018.70037
Vancouver ERGÜL M,ERGÜL M,ERUYGUR N,ATAŞ M,UÇAR E In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts. Turkish Journal of Pharmaceutical Sciences. 2019; 16(4): 401 - 409. 10.4274/tjps.galenos.2018.70037
IEEE ERGÜL M,ERGÜL M,ERUYGUR N,ATAŞ M,UÇAR E "In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts." Turkish Journal of Pharmaceutical Sciences, 16, ss.401 - 409, 2019. 10.4274/tjps.galenos.2018.70037
ISNAD ERGÜL, Mustafa vd. "In Vitro Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts". Turkish Journal of Pharmaceutical Sciences 16/4 (2019), 401-409. https://doi.org/10.4274/tjps.galenos.2018.70037
Sayfa Oluşturulma Tarihi
23-04-2024 13:1

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