Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods

AYDIN, Buket; ÜRKÜ, Çiğdem; AKAYLI, Tülay; KAYALAR, Ozgecan

doi:10.26650/EurJBiol.2018.404499

Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods

Tülay AKAYLI, (İstanbul Üniversitesi, Su Bilimleri Fakültesi, Balık Hastalıkları Anabilim Dalı, İstanbul, Türkiye)

Buket AYDIN, (İstanbul Üniversitesi, Su Bilimleri Fakültesi, Balık Hastalıkları Anabilim Dalı, İstanbul, Türkiye)

Çiğdem ÜRKÜ, (İstanbul Üniversitesi, Su Bilimleri Fakültesi, Balık Hastalıkları Anabilim Dalı, İstanbul, Türkiye)

Ozgecan KAYALAR (İstanbul Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, İstanbul, Türkiye)

European Journal of Biology

2 0

Yıl: 2018 Cilt: 77 Sayı: 1 Sayfa Aralığı: 26 - 31 Metin Dili: İngilizce DOI: 10.26650/EurJBiol.2018.404499 İndeks Tarihi: 05-10-2020

Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods

Öz:

ABSTRACTObjective: In this study, the diagnosis of infection caused by Vibrio anguillarum in cultured rainbow trout (Oncorhynchus mykiss), in Turkey using bacteriological, histopathological and immunohistochemical methods was attempted.Materials and Methods: 15 rainbow trout samples showing signs of disease were investigated using bacteriological and histopatological methods.Results: Because of physiological and biochemical characteristics of bacteria growing on the medium, these bacteria were isolated and identified as V. anguillarum. As a result of the histopathologic examination of the tissues of diseased rainbow trout, hyperemia in the liver, degeneration of the epithelium of kidney tubules and necrosis in the interstitial area, enlargement in the gill filaments, cells having picnotic nucleus and several necrotic areas in the spleen were observed. It was also detected in the streptavidin-biotin staining method that the causative agent accumulates in the liver, spleen, kidney and also in the gillsespecially in the areas near around the blood vessels depending on the development stage of the disease.Conclusion: As a result, an immunoenzimatic method, streptavidin-biotin staining method was used in this study for the detection of the presence of V. anguillarum in the infected rainbow trout tissue.

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APA	AKAYLI T, AYDIN B, ÜRKÜ Ç, KAYALAR O (2018). Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods. , 26 - 31. 10.26650/EurJBiol.2018.404499
Chicago	AKAYLI Tülay,AYDIN Buket,ÜRKÜ Çiğdem,KAYALAR Ozgecan Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods. (2018): 26 - 31. 10.26650/EurJBiol.2018.404499
MLA	AKAYLI Tülay,AYDIN Buket,ÜRKÜ Çiğdem,KAYALAR Ozgecan Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods. , 2018, ss.26 - 31. 10.26650/EurJBiol.2018.404499
AMA	AKAYLI T,AYDIN B,ÜRKÜ Ç,KAYALAR O Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods. . 2018; 26 - 31. 10.26650/EurJBiol.2018.404499
Vancouver	AKAYLI T,AYDIN B,ÜRKÜ Ç,KAYALAR O Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods. . 2018; 26 - 31. 10.26650/EurJBiol.2018.404499
IEEE	AKAYLI T,AYDIN B,ÜRKÜ Ç,KAYALAR O "Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods." , ss.26 - 31, 2018. 10.26650/EurJBiol.2018.404499
ISNAD	AKAYLI, Tülay vd. "Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods". (2018), 26-31. https://doi.org/10.26650/EurJBiol.2018.404499

APA	AKAYLI T, AYDIN B, ÜRKÜ Ç, KAYALAR O (2018). Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods. European Journal of Biology, 77(1), 26 - 31. 10.26650/EurJBiol.2018.404499
Chicago	AKAYLI Tülay,AYDIN Buket,ÜRKÜ Çiğdem,KAYALAR Ozgecan Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods. European Journal of Biology 77, no.1 (2018): 26 - 31. 10.26650/EurJBiol.2018.404499
MLA	AKAYLI Tülay,AYDIN Buket,ÜRKÜ Çiğdem,KAYALAR Ozgecan Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods. European Journal of Biology, vol.77, no.1, 2018, ss.26 - 31. 10.26650/EurJBiol.2018.404499
AMA	AKAYLI T,AYDIN B,ÜRKÜ Ç,KAYALAR O Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods. European Journal of Biology. 2018; 77(1): 26 - 31. 10.26650/EurJBiol.2018.404499
Vancouver	AKAYLI T,AYDIN B,ÜRKÜ Ç,KAYALAR O Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods. European Journal of Biology. 2018; 77(1): 26 - 31. 10.26650/EurJBiol.2018.404499
IEEE	AKAYLI T,AYDIN B,ÜRKÜ Ç,KAYALAR O "Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods." European Journal of Biology, 77, ss.26 - 31, 2018. 10.26650/EurJBiol.2018.404499
ISNAD	AKAYLI, Tülay vd. "Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods". European Journal of Biology 77/1 (2018), 26-31. https://doi.org/10.26650/EurJBiol.2018.404499