Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis
Yıl: 2020 Cilt: 26 Sayı: 4 Sayfa Aralığı: 483 - 490 Metin Dili: İngilizce DOI: DOI: 10.9775/kvfd.2019.23576 İndeks Tarihi: 08-11-2020
Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis
Öz: In this study, histologic and electromyographic (EMG) evaluation of neuroregenerative effect of stromal vascular fraction (SVF) following periferal nerve anastomosis was aimed. Totally, 31 Wistar Albino, male rats (weighing about 300 g) were studied, and these rats were grouped according to surgical techniques performed on the right sciatic nerve: group I (GRI) micro suture (n=7), group II (GRII) micro suture + SVF (n=7), group III (GRIII) fibrin glue (n=7) and group IV (GRIV) fibrin glue + SVF (n=7). Fat tissue was taken from 3 rats to prepare SVF, and SVF was produced by non-enzymatic method. The left sciatic nerve of all rats was evaluated for EMG as control. Under general anesthesia, after transversal incision of the sciatic nerve, microsurgical epineural repair technique was performed with 10/0 nonabsorbable suture. EMG examinations were performed in terms of conduction rate, amplitude, distal latency and spontaneous muscle activity at 0th day and postoperative (PO) 1st and 8th weeks. EMG results between and within the groups were statistically evaluated by one-way analysis of variance. Rats in all groups were sacrificed by decapitation at PO 8th week and histological examinations of the sciatic nerves were performed following preparation of the neural tissues. EMG examination results showed the highest nerve conduction in GRI, the highest amplitude in GRIII, normal latency in GRII and longer distal latency in GRIV at PO 8th week. Amplitude and conduction velocity increased gradually in all groups. In needle EMG, the best muscle membrane stabilization was achieved in GRII and GRIV at PO 8th week. Statistically, the values of amplitude, distal latency, conduction velocity, and spontaneous muscle activity were found to be at normal levels at PO 8th week in all groups (P>0.05). In the histological results, although fibroconnective tissue reactions in the anastomosis area had similar scores in GRII and GRIV, maximum fibroconnective tissue reaction and the best axonal regeneration was seen in GRI and GRIII, respectively. In addition, GRI and GRII had the most inflammatory cells accumulation in the suture region, and less inflammatory cells were seen in the anastomosis area of GRIII and GRIV. As a conclusion, fibrin glue presents good electrophysiological and histological results; however, it is clear that local SVF usage on the nerve anastomosis area can be a good choice to decrease fibroconnective tissue reaction and inflammation.
Anahtar Kelime: Nöroanastomoz Sonrası Stromal Vasküler Fraksiyonun Nörorejeneratif Etkisinin Histolojik ve Elektromiyografik Değerlendirilmesi
Öz: Bu çalışmada, periferal sinir anastomozunu takiben stromal vasküler fraksiyonun (SVF) nörorejeneratif etkisinin histolojik ve elektromiyografik (EMG) değerlendirilmesi amaçlandı. Toplamda, 31 Wistar Albino, erkek sıçanda (yaklaşık 300 gr ağırlığında) çalışıldı ve bu sıçanlar sağ siyatik sinirde uygulanan cerrahi tekniklere göre gruplandı: grup I (GRI) mikro dikiş (n=7), grup II (grup GRII) mikro dikiş + SVF (n=7), grup III (GRIII) fibrin yapıştırıcı (n=7) ve grup IV (GRIV) fibrin yapıştırıcı + SVF (n=7). SVF’yi hazırlamak için 3 sıçandan yağ dokusu alındı ve SVF, enzimatik olmayan bir yöntemle üretildi. Bütün sıçanların sol siyatik siniri EMG için kontrol olarak değerlendirildi. Genel anestezi altında, siyatik sinirin transversal ensizyonundan sonra 10/0 emilemeyen dikiş ile mikrocerrahi epinöral onarım tekniği uygulandı. EMG muayeneleri, 0. günde ve postoperatif (PO) 1. ve 8. haftalarda iletim hızı, amplitüt, distal latans ve spontan kas aktivitesi açısından yapıldı. EMG bulguları istatistiksel olarak gruplar arası ve grup içi tek yönlü varyans analizi ile değerlendirildi. Tüm gruplardaki sıçanlar PO 8. haftada dekapitasyon ile sakrifiye edildi ve sinir dokularının hazırlanmasından sonra siyatik sinirlerin histolojik incelemeleri yapıldı. EMG muayene sonuçları, GRI’de en yüksek sinir iletimini, GRIII’te en yüksek amplitütü, GRII’de normal latansı ve GRIV’te ameliyat sonrası 8. haftada daha uzun distal latansı gösterdi. Amplitüt ve iletim hızı, tüm gruplarda kademeli olarak arttı. İğne EMG’de, en iyi kas zarı stabilizasyonu PO 8. haftada GRII ve GRIV’te sağlandı. İstatistiksel olarak amplitüd, distal latans, ileti hızı ve spontan kas aktivitesi değerlerinin tüm gruplarda anlamlı olarak PO 8. haftada normal değerlerde olduğu görüldü (P>0.05). Histolojik sonuçlarda, anastomoz alanındaki fibrokonnektif doku reaksiyonları GRII ve GRIV’te benzer skorlara sahip olsa da, maksimum fibrokonnektif doku reaksiyonu ve en iyi aksonal rejenerasyon sırasıyla GRI ve GRIII’te görülmüştür. Ek olarak, GRI ve GRII dikiş bölgesinde en fazla enflamatuar hücre birikimine sahipti ve GRIII ve GRIV’ün anastomoz alanında daha az enflamatuar hücreler görüldü. Sonuç olarak, fibrin yapıştırıcısı iyi elektrofizyolojik ve histolojik sonuç sunar; ayrıca sinir anastomoz bölgesinde lokal SVF kullanımı fibrokonnektif doku reaksiyonu ve enflamasyonu azaltmak için iyi bir seçim olabileceği açıktır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Barton MJ, Morley JW, Stoodley MA, Lauto A, Mahns DA: Nerve repair: Toward a sutureless approach. Neurosurg Rev, 37 (4): 585-595, 2014. DOI: 10.1007/s10143-014-0559-1
- 2. Breshah MN, Sadakah AA, Eldrieny EA, Saad KA: Functional and histological evaluation of rat sciatic nerve anastomosis using cyanoacrylate and fibrin glue. Tanta Dent J, 10 (2): 67-74, 2013. DOI: 10.1016/j.tdj. 2013.08.005
- 3. Flores AJ, Lavernia CJ, Owens PW: Anatomy and physiology of peripheral nerve injury and repair. Am J Orthop (Belle Mead NJ), 29 (3): 167173, 2000.
- 4. Özaydın İ, Ünsaldı E, Aksoy Ö, Yayla S, Kaya M, Ulkay Tunalı MB, Aktaş A, Taşdemiroğlu E, Cihan M, Kurt B, Yıldırım HC, Şengöz A, Erdoğan H: The effect of silicone tube and silicone tube + hyaluronic acid application on adhesion formation in experimental peri- and epineurorrhaphy in a rat modal. Kafkas Univ Vet Fak Derg, 20 (4): 591-597, 2014. DOI: 10.9775/kvfd.2014.10583
- 5. Panagopoulos GN, Megaloikonomos PD, Mavrogenis AF: The present and future for peripheral nerve regeneration. Orthopedics, 40 (1): e141-e156, 2017. DOI: 10.3928/01477447-20161019-01
- 6. Rafijah G, Bowen AJ, Dolores C, Vitali R, Mozaffar T, Gupta R: The effect of adjuvant fibrin sealant on the surgical repair of segmental nerve defects in an animal model. J Hand Surg, 38 (5): 847-855, 2013. DOI: 10.1016/j. jhsa.2013.01.044
- 7. Wood MD, Kemp SWP, Weber C, Borschel GH, Gordon T: Outcome measures of peripheral nerve regeneration. Ann Anat, 193 (4): 321-333, 2011. DOI: 10.1016/j.aanat.2011.04.008
- 8. Bhandari PS: Use of fibrin glue in the repair of brachial plexus and peripheral nerve injuries. Indian J Neurotrauma, 10 (1): 30-32, 2013. DOI: 10.1016/j.ijnt.2013.05.008
- 9. Bhatnagar D, Bushman JS, Murthy NS, Merolli A, Kaplan HM, Kohn J: Fibrin glue as a stabilization strategy in peripheral nerve repair when using porpous nerve guidance conduits. J Mater Sci Mater Med, 28:79, 2017. DOI: 10.1007/s10856-017-5889-4
- 10. Childe JR, Regal S, Schimoler P, Kharlamov A, Miller MC, Tang P: Fibrin glue increases the tensile strength of conduit-assisted primary digital nerve repair. Hand, 13 (1): 45-49, 2018. DOI: 10.1177/1558944717691131
- 11. Suri A, Mehta VS, Sarkar C: Microneural anastomosis with fibrin glue: an experimental study. Neurol Indıa, 50 (1): 23-26, 2002.
- 12. Aksu AE, Çalış M: Yağ dokusundan elde edilen mezenkimal kök hücreler ve stromal vasküler fraksiyon konsepti. Türkiye Klinikleri J Plast Surg- Special Topics, 4 (3): 19-26, 2015.
- 13. Matsumine H, Numakura K, Climov M, Watanabe Y, Giatsidis G, Orgill DP: Facial-nerve regeneration ability of a hybrid artificial nerve conduit containing uncultured adipose-derived stromal vascular fraction: An experimental study. Microsurgery, 37 (7): 808-818, 2017. DOI: 10.1002/ micr.30060
- 14. Nguyen A, Guo J, Banyard DA, Fadavi D, Toranto JD, Wirth GA, Paydar KZ, Evans GRD, Widgerow AD: Stromal vascular fraction: A regenerative reality? Part 1: Current concepts and review of literature. J Plast Reconstr Aesthet Surg, 69 (2): 170-179, 2016. DOI: 10.1016/j.bjps.2015.10.015
- 15. Özkan HS, Silistreli ÖK, Ergür B, İrkören S: Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: An experimental study in rats. Ulus Travma Acil Cerrahi Derg, 22 (1): 7-11, 2016. DOI: 10.5505/tjtes.2015.12612
- 16. Gentile P, Scioli MG, Bielli A, Orlandi A, Cervelli V: Concise review: The use of adipose-derived stromal vascular fraction cells and platelet rich plasma in regenerative plastic surgery. Stem Cells, 35 (1): 117-134, 2017. DOI: 10.1002/stem.2498
- 17. Guo J, Nguyen A, Banyard DA, Fadavi D, Toranto JD, Wirth GA, Paydar KZ, Evans GRD, Widgerow AD: Stromal vascular fraction: A regenerative reality? Part 2: Mechanisms of regenerative action. J Plast Reconstr Aesthet Surg, 69 (2): 180-188, 2016. DOI: 10.1016/j.bjps.2015.10.014
- 18. Mohammadi R, Sanaei N, Ahsan S, Rostami H, Abbasipour-Dalivand S, Amini K: Repair of nerve defect with chitosan graft supplemented by uncultured characterized stromal vascular fraction in streptozotocin induced diabetic rats. Int J Surg, 12 (1): 33-40, 2014. DOI: 10.1016/j. ijsu.2013.10.018
- 19. Shimizu M, Matsumine H, Osaki H, Ueta Y, Tsunoda S, Kamei W, Hashimoto K, Niimi Y, Watanabe Y, Miyata M, Sakurai H: Adiposederived stem cells and the stromal vascular fraction in polyglycolic acidcollagen nerve conduits promote rat facial nerve regeneration. Wound Repair Regen, 26 (6): 446-455, 2018. DOI: 10.1111/wrr.12665
- 20. Bora P, Majumdar AS: Adipose tissue-derived stromal vascular fraction in regenerative medicine: A brief review on biology and translation. Stem Cell Res Ther, 8:145, 2017. DOI: 10.1186/s13287-017-0598-y
- 21. Harasymiak-Krzyzanowska I, Niedojadlo A, Karwat J, Kotuła L, Gil-Kulik P, Sawiuk M, Kocki J: Adipose tissue-derived stem cells show conciderable promise for regenerative medicine applications. Cell Mol Biol Lett, 18, 479-493, 2013. DOI: 10.2478/s11658-013-0101-4
- 22. Mohammadi R, Azizi S, Delirez N, Hobbenaghi R, Amini K: Transplantation of uncultured omental adipose-derived stromal vascular fraction improves sciatic nerve regeneration and functional recovery through inside-out vein graft in rats. J Trauma Acute Care Surg, 72 (2): 390396, 2012. DOI: 10.1097/ta.0b013e31821181dd
- 23. Mohammadi R, Mehrtash M, Mehrtash M, Sajjadi SS: Nonexpanded adipose stromal vascular fraction local therapy on peripheral nerve regeneration using allografts. J Invest Surg, 29 (3): 149-156, 2016. DOI: 10.3109/08941939.2015.1093046
- 24. Öge AE, Matur Z: Sinir Iletim Çalışmaları ve Elektromiyografi Atlası. 2025, Nobel Yayınevi, İstanbul, 2013.
- 25. Shah FS, Wu X, Dietrich M, Rood J, Gimble JM: A non-enzymatic method for isolating human adipose tissue-derived stromal stem cells. Cytotherapy, 15 (8): 979-985, 2013. DOI: 10.1016/j.jcyt.2013.04.001
- 26. Li YS, Chen PJ, Wu LW, Chou PW, Sun LY, Chiou TW: Investigating the mincing method for isolation of adipose-derived stem cells from pregnant women fat. Cytotechnology, 70 (1): 55-66, 2018. DOI: 10.1007/s10616-0170162-8
- 27. Crossmann G: A modification of Mallory’s connective tissue stain with a discussion of the principles involved. Anat Rec, 69, 33-38, 1937. DOI: 10.1002/ar.1090690105
- 28. Adams EJ, Gren JA, Clark AH, Youngson JH: Comparison of different scoring systems for immunohistochemical staining. J Clin Pathol, 52, 75-77, 1999. DOI: 10.1136/jcp.52.1.75
- 29. Wolthers M, Moldowan M, Binderup T, Schmalbruch H, Krarup C: Comparative electrophysiological, functional, and histological studies of nerve lesions in rats. Microsurgery, 25 (6): 508-519, 2005. DOI: 10.1002/ micr.20156
- 30. Mohammadi R, Sanaei N, Ahsan S, Masoumi-Verki M, Khadir F, Mokarizadeh A: Stromal vascular fraction combined with silicone rubber chamber improves sciatic nerve regeneration in diabetes. Chin J Traumatol, 18 (4): 212-218, 2015. DOI: 10.1016/j.cjtee.2014.10.005
- 31. Menovsky T, Beek JF: Laser, fibrin glue, or suture repair of peripheral nerves: A comparative functional, histological and morohometric study in the rat sciatic nerve. J Neurosurg, 95 (4): 694-699, 2001. DOI: 10.3171/ jns.2001.95.4.0694
- 32. Shamel J, Meyer VE, Bachem U: Glueing of peripheral nerves with fibrin: Experimental studies. J Reconstr Microsurg, 3 (3): 211-218, 1987. DOI: 10.1055/s-2007-1006987
- 33. Gramsbergen A, Ijkema-Paassen J, Meek MF: Sciatic nerve transection in the adult rat: Abnormal EMG patterns during locomotion by aberrant innervation of hindleg muscles. Exp Neurol, 161 (1): 183-193, 2000. DOI: 10.1006/exnr.1999.7233
- 34. Georgiou M, Golding JP, Loughlin AJ, Kingham PJ, Phillips JB: Engineered neural tissue with aligned, differentiated adipose-derived stem cells promotes peripheral nerve regeneration across a critical sized defect in rat sciatic nerve. Biomaterials, 37, 242-251, 2015. DOI: 10.1016/j. biomaterials.2014.10.009
- 35. Isaacs JE, McDaniel CO, Owen JR, Wayne JS: Comparative analysis of biomechanical performance of available “nerve glues”. J Hand Surg, 33 (6): 893-899, 2008. DOI: 10.1016/j.jhsa.2008.02.009
- 36. Martins RS, Siqueira MG, Da Silva CF, Plese JP: Overall assessment of regeneration in peripheral nerve lesion repair using fibrin glue, suture, or a combination of the 2 techniques in a rat model. Which is the ideal choice? Surg Neurol, 64, S1:10-S1:16, 2005. DOI: 10.1016/j.surneu.2005.04.022
- 37. Hüseyinoğlu N, Özaydın İ, Yayla S, Yıldırım CH, Aksoy Ö, Kaya M, Şengöz A, Taşdemiroğlu E: Electrophysiological assessment of the effects of silicone tubes and hyaluronic acid on nerve regeneration in rats with sciatic neurorrhaphy. Kafkas Univ Vet Fak Derg, 18 (6): 917-922, 2012. DOI: 10.9775/kvfd.2012.6373
- 38. Sta M, Cappaert NLM, Ramekers D, Baas F, Wadman WJ: The functional and morphological characteristics of sciatic nerve degeneration and regeneration after crush injury in rats. J Neurosci Methods, 222, 189-198, 2014. DOI: 10.1016/j.jneumeth.2013.11.012
- 39. Martins RS, Siqueira MG, Da Silva CF, De Godoy BO, Plese JPP: Electrophysiologic assessment of regeneration in rat sciatic nerve repair using suture, fibrin glue or a combination of both techniques. Arq Neuropsiquiatr, 63 (3-A): 601-604, 2005. DOI: 10.1590/s0004-282x2005000400009
| APA | Acar H, ÇERÇİ E, karimi khezri m, ÇETİN M, canatan u, SEN M, ASLAN V, Altıncı Sarıl C, MEKİK TEMİZ E, Taskapilioglu M, ERDOST H, SALCI H (2020). Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis. , 483 - 490. DOI: 10.9775/kvfd.2019.23576 |
| Chicago | Acar Hilal,ÇERÇİ Ece,karimi khezri marzieh,ÇETİN Melike,canatan uygur,SEN Mehmet Metin,ASLAN Vildan,Altıncı Sarıl Canan,MEKİK TEMİZ ELİF,Taskapilioglu M Ozgur,ERDOST Hatice,SALCI Hakan Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis. (2020): 483 - 490. DOI: 10.9775/kvfd.2019.23576 |
| MLA | Acar Hilal,ÇERÇİ Ece,karimi khezri marzieh,ÇETİN Melike,canatan uygur,SEN Mehmet Metin,ASLAN Vildan,Altıncı Sarıl Canan,MEKİK TEMİZ ELİF,Taskapilioglu M Ozgur,ERDOST Hatice,SALCI Hakan Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis. , 2020, ss.483 - 490. DOI: 10.9775/kvfd.2019.23576 |
| AMA | Acar H,ÇERÇİ E,karimi khezri m,ÇETİN M,canatan u,SEN M,ASLAN V,Altıncı Sarıl C,MEKİK TEMİZ E,Taskapilioglu M,ERDOST H,SALCI H Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis. . 2020; 483 - 490. DOI: 10.9775/kvfd.2019.23576 |
| Vancouver | Acar H,ÇERÇİ E,karimi khezri m,ÇETİN M,canatan u,SEN M,ASLAN V,Altıncı Sarıl C,MEKİK TEMİZ E,Taskapilioglu M,ERDOST H,SALCI H Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis. . 2020; 483 - 490. DOI: 10.9775/kvfd.2019.23576 |
| IEEE | Acar H,ÇERÇİ E,karimi khezri m,ÇETİN M,canatan u,SEN M,ASLAN V,Altıncı Sarıl C,MEKİK TEMİZ E,Taskapilioglu M,ERDOST H,SALCI H "Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis." , ss.483 - 490, 2020. DOI: 10.9775/kvfd.2019.23576 |
| ISNAD | Acar, Hilal vd. "Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis". (2020), 483-490. https://doi.org/DOI: 10.9775/kvfd.2019.23576 |
| APA | Acar H, ÇERÇİ E, karimi khezri m, ÇETİN M, canatan u, SEN M, ASLAN V, Altıncı Sarıl C, MEKİK TEMİZ E, Taskapilioglu M, ERDOST H, SALCI H (2020). Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(4), 483 - 490. DOI: 10.9775/kvfd.2019.23576 |
| Chicago | Acar Hilal,ÇERÇİ Ece,karimi khezri marzieh,ÇETİN Melike,canatan uygur,SEN Mehmet Metin,ASLAN Vildan,Altıncı Sarıl Canan,MEKİK TEMİZ ELİF,Taskapilioglu M Ozgur,ERDOST Hatice,SALCI Hakan Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26, no.4 (2020): 483 - 490. DOI: 10.9775/kvfd.2019.23576 |
| MLA | Acar Hilal,ÇERÇİ Ece,karimi khezri marzieh,ÇETİN Melike,canatan uygur,SEN Mehmet Metin,ASLAN Vildan,Altıncı Sarıl Canan,MEKİK TEMİZ ELİF,Taskapilioglu M Ozgur,ERDOST Hatice,SALCI Hakan Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.26, no.4, 2020, ss.483 - 490. DOI: 10.9775/kvfd.2019.23576 |
| AMA | Acar H,ÇERÇİ E,karimi khezri m,ÇETİN M,canatan u,SEN M,ASLAN V,Altıncı Sarıl C,MEKİK TEMİZ E,Taskapilioglu M,ERDOST H,SALCI H Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(4): 483 - 490. DOI: 10.9775/kvfd.2019.23576 |
| Vancouver | Acar H,ÇERÇİ E,karimi khezri m,ÇETİN M,canatan u,SEN M,ASLAN V,Altıncı Sarıl C,MEKİK TEMİZ E,Taskapilioglu M,ERDOST H,SALCI H Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(4): 483 - 490. DOI: 10.9775/kvfd.2019.23576 |
| IEEE | Acar H,ÇERÇİ E,karimi khezri m,ÇETİN M,canatan u,SEN M,ASLAN V,Altıncı Sarıl C,MEKİK TEMİZ E,Taskapilioglu M,ERDOST H,SALCI H "Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26, ss.483 - 490, 2020. DOI: 10.9775/kvfd.2019.23576 |
| ISNAD | Acar, Hilal vd. "Histologic and Electromyographic Evaluation of Neuroregenerative Effect of Stromal Vascular Fraction Following Neuroanastomosis". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26/4 (2020), 483-490. https://doi.org/DOI: 10.9775/kvfd.2019.23576 |
