Despite widespread traditional usage of Eryngium species in Anatolia (Turkey), only a limited number ofscientific studies exists on E. kotschyi, an endemic species. Previously, extracts from E. campestre and E. kotschyi werereported to have significant anti-inflammatory effects in vivo. This study aimed to investigate the in vitro antiinflammatory, antioxidant, urease inhibitory activities of ethanol extracts of E. kotschyi and E. campestre var. virens rootsas well as distilled water and ethanol extracts of E. kotschyi aerial parts. The NO and cytokine inhibitory effects wereevaluated by Griess and ELISA assays. The antioxidant activities were tested on DPPH•, ABTS•+ and CUPRAC assays.The EtOH extract of E. kotschyi roots (EKr EtOH) and aerial parts (EKae EtOH) inhibited 50.08% and 41.52% of NOproduction at 100 µg/ml, respectively. The EtOH extract of the roots of E. campestre var. virens (ECr EtOH) and EKrEtOH provided 36.22% and 65.23% IL-6 inhibition and 44.24% and 56.84% IL-1α inhibition at 100 µg/ml. EKae EtOHexerted highest antioxidant activity on ABTS•+ (2.4±0.0005 µM trolox/mg extract) and CUPRAC (0.97±0.07 mMtrolox/mg extract). This extract was also found the richest among all in terms of phenolics content (6.1±0.001mg/GAE/g extract). EKr EtOH and ECr EtOH exhibited strongest DPPH• (IC50 = 1.132±0.057 mg/ml) radicalscavenging and ferric reducing/antioxidant power (0.36±0.005 mM Fe2+/mg extract) activity respectively. The extractsexerted low urease inhibitory activity. Consequently, the results of this study might contribute to the elucidation of themechanism of in vivo anti-inflammatory activity of the extracts.