Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars
Yıl: 2020 Cilt: 60 Sayı: 2 Sayfa Aralığı: 37 - 44 Metin Dili: İngilizce DOI: 10.16955/bitkorb.639801 İndeks Tarihi: 25-11-2020
Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars
Öz: Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 is a multihost plantpathogenic bacterium all over the world and also in Turkey. The aim of this studywas to identify the pathogenic bacteria causing the symptoms of water-soakednecrotic leaf spots, dark brown spots on leaves, tip burn of leaf in curly type lettuce(Lactuca sativa var. crispy) grown in the field conditions in Beypazarı regionand to reveal the reactions of some lettuce cultivars against the bacteria. For thispurpose, twelve isolates were obtained from 4 different locations. Pathogenicitytests were conducted on curly type lettuce and all isolates were found pathogenicon the host. Isolated bacteria were evaluated for Gram reaction, LOPAT tests,colony morphology on King’s Medium B and carbon sources utilization byVITEK-2 system and identified as Pseudomonas viridiflava. The 16S rDNA regionof a selected isolate representing the similar isolates in all tests was sequenced forgenetic confirmation and the obtained sequence was submitted to the GeneBankunder the accession number MN_539659. The result of the obtained sequence was99.75% similar to the respective reference sequence after BLAST analysis. Reactionof the three different widely produced lettuce types, iceberg, curly and romainetypes, with ten varieties against the causative bacterial agent was evaluated andthe difference between the varieties found significant statistically. In this study, P.viridiflava was detected in lettuce in Central Anatolia for the first time.
Anahtar Kelime: Marul yaprak lekesi etmeni Pseudomonas viridiflava (Burkholder 1930) Dowson’nın tespiti ve bazı marul çeşitlerinin reaksiyonlarının belirlenmesi
Öz: Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 tüm dünyada ve Türkiye’de çok konukçulu bitki patojeni bir bakteridir. Beypazarı ilçesinde tarla koşullarında yetiştirilen kıvırcık tip marullarda sulumsu nekrotik lekeler, yapraklarda koyu kahverengi lekeler ve yaprak uçlarında yanıklık belirtileri bakterinin saptanması için incelenmiştir. Dört farklı tarladan 12 izolat elde edilmiştir. Patojenite testleri kıvırcık tip marul üzerinde yapılmış ve ele alınan tüm izolatlar konukçusu üzerinde patojen bulunmuştur. İzole edilen bakteriler Gram reaksiyonu, LOPAT testleri, King B besi yerinde oluşturduğu koloni morfolojisi ve VITEK-2 sistemi ile karbon kaynakları kullanımı yönüyle değerlendirilmiş ve P. viridiflava olarak tespit edilmişlerdir. Tüm testlerde benzerlik gösteren izolatları temsilen seçilen bir izolatın 16S rDNA bölgesi genetik doğrulama için sekanslanmış ve elde edilen dizi GenBank’a MN_539659 kodu ile kaydedilmiştir. Elde edilen sekans dizisinin sonucu BLAST analizi sonrası referans dizi ile %99.75 benzerlik oranı göstermiştir. Yaygın olarak üretimi yapılan iceberg, kıvırcık ve yedikule tipi üç farklı marul tipinden toplam on çeşit ile etmene karşı çeşitlerin reaksiyonu değerlendirilmiş olup çeşitler arasındaki fark istatistiksel olarak önemli bulunmuştur. Bu çalışma ile İç Anadolu Bölgesi’nde P.viridiflava marullarda ilk kez tespit edilmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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- Cite this article: Canik Orel, D. (2020). Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. Plant Protection Bulletin, 60-2. DOI: 10.16955/bitkorb.639801
- Atıf için: Canik Orel, D. (2020). Marul yaprak lekesi etmeni Pseudomonas viridiflava (Burkholder 1930) Dowson’nın tespiti ve bazı marul çeşitlerinin reaksiyonlarının belirlenmesi. Bitki Koruma Bülteni, 60-2. DOI: 10.16955/ bitkorb.639801
| APA | OREL D (2020). Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. , 37 - 44. 10.16955/bitkorb.639801 |
| Chicago | OREL Didem CANİK Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. (2020): 37 - 44. 10.16955/bitkorb.639801 |
| MLA | OREL Didem CANİK Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. , 2020, ss.37 - 44. 10.16955/bitkorb.639801 |
| AMA | OREL D Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. . 2020; 37 - 44. 10.16955/bitkorb.639801 |
| Vancouver | OREL D Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. . 2020; 37 - 44. 10.16955/bitkorb.639801 |
| IEEE | OREL D "Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars." , ss.37 - 44, 2020. 10.16955/bitkorb.639801 |
| ISNAD | OREL, Didem CANİK. "Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars". (2020), 37-44. https://doi.org/10.16955/bitkorb.639801 |
| APA | OREL D (2020). Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. Bitki Koruma Bülteni, 60(2), 37 - 44. 10.16955/bitkorb.639801 |
| Chicago | OREL Didem CANİK Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. Bitki Koruma Bülteni 60, no.2 (2020): 37 - 44. 10.16955/bitkorb.639801 |
| MLA | OREL Didem CANİK Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. Bitki Koruma Bülteni, vol.60, no.2, 2020, ss.37 - 44. 10.16955/bitkorb.639801 |
| AMA | OREL D Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. Bitki Koruma Bülteni. 2020; 60(2): 37 - 44. 10.16955/bitkorb.639801 |
| Vancouver | OREL D Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars. Bitki Koruma Bülteni. 2020; 60(2): 37 - 44. 10.16955/bitkorb.639801 |
| IEEE | OREL D "Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars." Bitki Koruma Bülteni, 60, ss.37 - 44, 2020. 10.16955/bitkorb.639801 |
| ISNAD | OREL, Didem CANİK. "Determination of bacterial leaf spot of lettuce caused by Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 and the reactions of some lettuce cultivars". Bitki Koruma Bülteni 60/2 (2020), 37-44. https://doi.org/10.16955/bitkorb.639801 |
