In vitro antifungal activity of strains of Trichoderma harzianum

KIVANÇ, Merih; KÜÇÜK, Çiğdem

In vitro antifungal activity of strains of Trichoderma harzianum

Çiğdem KÜÇÜK, (Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Merih KIVANÇ (Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Turkish Journal of Biology

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Yıl: 2004 Cilt: 28 Sayı: 2-4 Sayfa Aralığı: 111 - 115 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

In vitro antifungal activity of strains of Trichoderma harzianum

Öz:

Interactions between Trichoderma harzianum strains and some soilborne plant pathogens (Gaeumannomyces graminis var. tritici, Fusarium culmorum and F. moniliforme) were studied on PDA medium. All T. harzianum strains tested produced a metabolite that inhibited growth of plant pathogenic fungi on PDA medium. When grown in liquid cultures containing laminarin, chitin or fungal cell walls as sole carbon sources, 2 strains of T. harzianum produced 1,3-p-glucanase and chitinase in the medium. Higher levels of these enzymes were induced by T. harzianum T15.

Anahtar Kelime: enzyme activity beta-glucanase cell walls chitinase soilborne pathogens Trichoderma harzianum plant pathogenic fungi

Konular: Biyoloji

Trichoderma harzianum izolatlarının in vitro antifungal aktivitesi

Öz:

PDA gelişme ortamında, bazı toprak kökenli bitki patojenleri (Gaeumannomyces graminis var. tritici, Fusarium culmorum ve F. moniliforme) ve Trichoderma harzianum streynleri arasındaki interaksiyonlar çalışılmıştır. Test edilen tüm Trichoderma harzianum streynleri, bitki patojeni fungusların gelişimini PDA ortamında inhibe eden uçucu metabolit üretmişlerdir. Karbon kaynağı olarak laminarin, kitin veya fungal hücre duvarı içeren sıvı ortamda geliştirildiklerinde, Trichoderma harzianum 'un iki streyni ortamda l ,3-p-glukanaz ve kitinaz enzimleri üretmişlerdir. Bu enzimlerin en yüksek düzeyleri Trichoderma harzianum Tl 5 tarafından üretilmiştir.

Anahtar Kelime: kitinaz toprak kaynaklı patojenler Trichoderma harzianum bitki patojen mantarlar enzim aktivitesi beta-glukanaz hücre duvarı

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

TRDizin Dışındaki Atıf Sayıları

OpenCitations: 0

OpenAlex: 0

Crossref: 0

1. Cook FU, Vesth FU. Wheat Health management. American Phytopathological Society, St. Paul, MN. 1991
2. Baker KF, Cook RJ. The nature and practice of biological control of plant pathogens, American Phytopathological Society, St Paul, MN. 1983.
3. Alabouvette C, Rouxel F, Louvet J. Characteristics of Fusarium wilt-suppressive soils and prospects for their utilization in biological control. In: Schippers B, Gams W (Eds) Soilborne Plant Pathogens. Academic Press, London, 648 pp. 1979.
4. Elad Y, Chet I, Henis Y. Degradation of plant pathogenic fungi by Trichoderma harzianum. Can J Microbiol 28: 719-725. 1982.
5. Sivan, A, Chet 1. Biological control of Fusarium spp in cotton, wheat and muskmelon by Trichoderma harzianum. J Phytopathol 116:39-47.1986. 13.
6. Küçük Ç, Kıvanç M. Isolation of Trichoderma spp. and their antifungal, biochemical and physiological features. Turk J Biol. 27:247-253.2003.
7. Simon C, Sivasithamparam M. Interactions among G. graminis var tritici, Trichoderma koningii and soil bacteria. Can J Microbiol 34: 871-876. 1988.
8. Schirmbock M, Lorito M, Wang YL et al. Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathdgenic fungi. Appl Environ Microbiol 60: 4344-4370. 1994.
9. Inbar J, Abransky D, Cohen D et al. Plant growth enhancement and disease control by Trichoderma harzianum in vegetable seedlings grown under commercial conditions. Eur J Plant Pathol 100:337-346. 1994.
10. Chet I, Henis Y, Mitchell R. Chemical composition of hyphal and sclerotial walls of Sclerotium rolfsii Sacc. Can J Microbiol 13: 137-141. 1967. 11. Cherif M, Benhamou N. Cytochemical aspect of chitin breakdown during the parasitic action of a Trichoderma sp. on F. oxysporum f. sp. radicis-lycopersici. J Phytopathol 80: 1406-1414. 1990.
12. Dennis C, Webster J. Antagonistic properties of species groups of Trichoderma III. Hyphal Interaction. Trans. British Mycological Society, 57: 363-369. 1971.
13. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254. 1976.
14. Sivan A, Chet I. Degradation of fungal cell walls by lytic enzymes of Trichoderma harzianum. J Gen Microbiol 135: 675-682. 1989.

APA	KÜÇÜK Ç, KIVANÇ M (2004). In vitro antifungal activity of strains of Trichoderma harzianum. , 111 - 115.
Chicago	KÜÇÜK Çiğdem,KIVANÇ Merih In vitro antifungal activity of strains of Trichoderma harzianum. (2004): 111 - 115.
MLA	KÜÇÜK Çiğdem,KIVANÇ Merih In vitro antifungal activity of strains of Trichoderma harzianum. , 2004, ss.111 - 115.
AMA	KÜÇÜK Ç,KIVANÇ M In vitro antifungal activity of strains of Trichoderma harzianum. . 2004; 111 - 115.
Vancouver	KÜÇÜK Ç,KIVANÇ M In vitro antifungal activity of strains of Trichoderma harzianum. . 2004; 111 - 115.
IEEE	KÜÇÜK Ç,KIVANÇ M "In vitro antifungal activity of strains of Trichoderma harzianum." , ss.111 - 115, 2004.
ISNAD	KÜÇÜK, Çiğdem - KIVANÇ, Merih. "In vitro antifungal activity of strains of Trichoderma harzianum". (2004), 111-115.

APA	KÜÇÜK Ç, KIVANÇ M (2004). In vitro antifungal activity of strains of Trichoderma harzianum. Turkish Journal of Biology, 28(2-4), 111 - 115.
Chicago	KÜÇÜK Çiğdem,KIVANÇ Merih In vitro antifungal activity of strains of Trichoderma harzianum. Turkish Journal of Biology 28, no.2-4 (2004): 111 - 115.
MLA	KÜÇÜK Çiğdem,KIVANÇ Merih In vitro antifungal activity of strains of Trichoderma harzianum. Turkish Journal of Biology, vol.28, no.2-4, 2004, ss.111 - 115.
AMA	KÜÇÜK Ç,KIVANÇ M In vitro antifungal activity of strains of Trichoderma harzianum. Turkish Journal of Biology. 2004; 28(2-4): 111 - 115.
Vancouver	KÜÇÜK Ç,KIVANÇ M In vitro antifungal activity of strains of Trichoderma harzianum. Turkish Journal of Biology. 2004; 28(2-4): 111 - 115.
IEEE	KÜÇÜK Ç,KIVANÇ M "In vitro antifungal activity of strains of Trichoderma harzianum." Turkish Journal of Biology, 28, ss.111 - 115, 2004.
ISNAD	KÜÇÜK, Çiğdem - KIVANÇ, Merih. "In vitro antifungal activity of strains of Trichoderma harzianum". Turkish Journal of Biology 28/2-4 (2004), 111-115.