The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey

KARAPINAR, Zeynep; OZKAN, CUMALI; DİNÇER, Ender

The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey

Zeynep KARAPINAR, (Balıkesir Üniversitesi, Veteriner Fakültesi, Viroloji Ana Bilim Dalı, Balıkesir, Türkiye)

Ender DİNÇER, (Mersin Üniversitesi, İleri Teknoloji Eğitim Araştırma ve Uygulama Merkezi, Mersin, Türkiye)

Cumali ÖZKAN (Van Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Ana Bilim Dalı, Van, Türkiye)

Van Veterinary Journal

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Yıl: 2018 Cilt: 29 Sayı: 2 Sayfa Aralığı: 83 - 86 Metin Dili: İngilizce İndeks Tarihi: 28-04-2021

The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey

Öz:

In this study, it was aimed to determine the presence of CPV infection in the blood and rectal swaps of suspected dogs that they were handed to Yuzuncu YılUniversity, Faculty of Veterinary, Department of Internal Disease using PCR techniques and molecular characterization of local viruses. 57 (75%) of the 76 rectal swabs used in the study and 40 (64%) of the 62 blood samples were determined to be positive by PCR obtained from dogs with clinical symptoms. Selected samples were positive with the virus carried the molecular characterization. The results of the sequence analysis were similar among themselves and they were found in CPV-2a and CPV-2b groups. It was determined that the sequences of virus strains showed 99-100% homology with other strains obtained from Genbank.

Anahtar Kelime:

Türkiyede Van Yöresinde Bulunan Köpeklerden Sağlanan Kan veRektal Sıvap Örneklerinde Canine Parvovirus-2 Enfeksiyonunun Araştırılması ve Filogenetik Analizi

Öz:

Bu çalışmada, Van ili Yüzüncü Yıl Üniversitesi Veteriner Fakültesi İç Hastalıkları Kliniğine getirilenCanine Parvovirus (CPV) Enfeksiyonu şüpheli köpeklerden alınan kan ve rektal sıvap örneklerinde PZR tekniği kullanılarak CPV enfeksiyonunun varlığının belirlenmesi ve yerel virusların moleküler karakterizasyonunun yapılması amaçlandı. Klinik olarak enfeksiyon belirtisi gösteren köpeklerden sağlanan 76 adet rektal sıvaptan 57 adedi (%75), 62 adet kan örneğinin ise 40 adedi (%64) PZR ile pozitif olarak belirlendi. Seçilen pozitif örnekler ile virusun moleküler karakterizasyonu gerçekleştirildi. Elde edilen sekans sonucu bilgileri değerlendirildiğinde örneklerin CPV-2a ve CPV-2b grublarında bulunduğu ve kendi aralarında benzerlik gösterdikleri belirlendi.Elde edilen virus suşu sekanslarının Genbankasından sağlanan diğer suşlarla %99-100 oranında homoloji gösterdiği belirlendi.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARAPINAR Z, DİNÇER E, OZKAN C (2018). The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey. , 83 - 86.
Chicago	KARAPINAR Zeynep,DİNÇER Ender,OZKAN CUMALI The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey. (2018): 83 - 86.
MLA	KARAPINAR Zeynep,DİNÇER Ender,OZKAN CUMALI The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey. , 2018, ss.83 - 86.
AMA	KARAPINAR Z,DİNÇER E,OZKAN C The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey. . 2018; 83 - 86.
Vancouver	KARAPINAR Z,DİNÇER E,OZKAN C The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey. . 2018; 83 - 86.
IEEE	KARAPINAR Z,DİNÇER E,OZKAN C "The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey." , ss.83 - 86, 2018.
ISNAD	KARAPINAR, Zeynep vd. "The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey". (2018), 83-86.

APA	KARAPINAR Z, DİNÇER E, OZKAN C (2018). The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey. Van Veterinary Journal, 29(2), 83 - 86.
Chicago	KARAPINAR Zeynep,DİNÇER Ender,OZKAN CUMALI The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey. Van Veterinary Journal 29, no.2 (2018): 83 - 86.
MLA	KARAPINAR Zeynep,DİNÇER Ender,OZKAN CUMALI The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey. Van Veterinary Journal, vol.29, no.2, 2018, ss.83 - 86.
AMA	KARAPINAR Z,DİNÇER E,OZKAN C The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey. Van Veterinary Journal. 2018; 29(2): 83 - 86.
Vancouver	KARAPINAR Z,DİNÇER E,OZKAN C The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey. Van Veterinary Journal. 2018; 29(2): 83 - 86.
IEEE	KARAPINAR Z,DİNÇER E,OZKAN C "The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey." Van Veterinary Journal, 29, ss.83 - 86, 2018.
ISNAD	KARAPINAR, Zeynep vd. "The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal SwabSamples from Dogs in Van Province, Turkey". Van Veterinary Journal 29/2 (2018), 83-86.