Investigation of Vector-Borne Diseases in Dogs
Yıl: 2020 Cilt: 9 Sayı: 2 Sayfa Aralığı: 154 - 160 Metin Dili: İngilizce DOI: 10.31196/huvfd.792147 İndeks Tarihi: 02-05-2021
Investigation of Vector-Borne Diseases in Dogs
Öz: In this study, a total of 186 blood samples were collected from kennel dogs consisting of 104 male and 82 female in five provinces (Mersin, Adana, Hatay, Gaziantep and Batman) of Turkey, and evaluated using molecular methods for the presence of canine vector-borne diseases (CVBDs). Overall, 10.8% of the sampled dogs were found to be infected with one or more CVBD pathogens investigated. Ehrlichia canis (17/186; 9.1%) was the most common CVBD pathogen, followed by Babesia canis vogeli (5/186; 2.7%) and Hepatozoon canis (1/186; 0.5%), respectively. Co-infection of E. canis with B. caniswas detected in 3 (1.6%) dogs. Infection with Rickettsia spp., Coxiella burnetii, Borrelia burgdorferi s.l., Francisella tularensis, Bartonella spp., Leishmania spp., Diroflaria immitis, Diroflaria repens, and Acanthocheilonema reconditum were not detected. No sex association with CVBDs was determined (p>0.05). The result of the study indicates the presence of three CVB pathogens, including the first report of B. canis and H. canis in the studied provinces.
Anahtar Kelime: Köpeklerde Vektör Kaynaklı Hastalıkların Araştırılması
Öz: Bu çalışmada, Türkiye'nin beş farklı ilindeki (Mersin, Adana, Hatay, Gaziantep ve Batman) köpek barınaklarındanalınan 186 (104'ü erkek ve 82'si dişi) kan örneği vektör kaynaklı nakledilen patojenler yönünden moleküler yöntemlerlearaştırıldı. İncelenen örneklerin %10.8'inin en az bir veya birden fazla patojen ile enfekte olduğu tespit edildi. Ehrlichiacanis (17/186; %9.1) en yaygın vektör aracılı nakledilen patojen olup, bunu sırasıyla Babesia canis vogeli (5/186; %2.7) veHepatozoon canis (1/186; %0.5) izledi. E. canis ve B. canis ortak enfeksiyonu 3 (%1.6) köpekte tespit edildi. Rickettsia spp.,Coxiella burnetii, Borrelia burgdorferi s.l., Francisella tularensis, Bartonella spp., Leishmania spp., Diroflaria immitis,Diroflaria repens ve Acanthocheilonema reconditum enfeksiyonu saptanmadı. Vektör aracılı nakledilen patojenler yönündenpozitif bulunan köpeklerde yaş ve cinsiyet yönünden istatistiksel olarak önemli bir fark belirlenmedi (p> 0.05). Çalışılanillerde köpeklerde vektör aracılı nakledilen patojenlerden üçünün varlığı gösterilmiş ve çalışılan illerde ilk kez B. canis ve H.canis varlığı tespit edilmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Aktaş M, Özübek S, Sayın Ipek DN, 2013: Molecular investigations of Hepatozoon species in dogs and developmental stages of Rhipicephalus sanguineus. Parasitol Res, 112, 2381-2385.
- Aktaş M, Özübek S, Altay K, Sayın İpek ND, Balkaya İ, Utuk AE, Kırbaş A, Şimsek S, Dumanlı N, 2015a: Molecular detection of tick-borne rickettsial and protozoan pathogens in domestic dogs from Turkey. Parasites & Vectors, 8, 157.
- Aktaş M, Özübek S, Altay K , Balkaya İ, Utuk AE, Kırbaş A, Şimsek S, Dumanlı N, 2015b: A molecular and parasitological survey of Hepatozoon canis in domestic dogs in Turkey. Vet Parasitol, 209, 264- 267.
- Aktaş M, 2014: A survey of ixodid tick species and molecular identification of tick-borne pathogens. Vet Parasitol, 200, 276–283.
- Aktaş M, Özübek S, İpek DN, 2013: Molecular investigations of Hepatozoon species in dogs and developmental stages of Rhipicephalus sanguineus. Parasitol Res, 112, 2381–2385.
- Aysul N, 2006: Comparison of microscopic and PCR-RLB findings in detection of Babesia species of dogs in Istanbul. PhD Thesis, Istanbul University, Institute of Health Science, Parasitology Department, Istanbul, Turkey.
- Baneth G, 2011: Perspectives on canine and feline hepatozoonosis. Vet Parasitol, 181, 3–11.
- Baneth G, Bourdeau P, Bourdoiseau G, Bowman D, Breitschwerdt E, Capelli G, Cardoso L, Dantas-Torres F, Day M,Dedet JP,Dobler G, Ferrer L, Irwin P, et al., 2012: Vector-Borne Diseases-constant challenge for practicing veterinarians: recommendations from the CVBD World Forum. Parasit & Vectors, 5, 55.
- Bell CA, Patel R, 2005: A real-time combined polymerase chain reaction assay for the rapid detection and differentiation of Anaplasma phagocytophilum, Ehrlichia chaffeensis and Ehrlichia ewingii. Diagn Microbiol Infect Dis, 53, 301–306.
- Bouza-Mora L, Dolz G, Solórzano-Morales A, Romero Zuñiga JJ, Salazar-Sánchez L, Labruna MB, Aguiar DM, 2017: Novel genotype of Ehrlichia canis detected in samples of human blood bank donors in Costa Rica. Ticks Tick Borne Dis, 8, 36-40.
- Bölükbaş CS, Pekmezci D, Gürler AT, Pekmezci GZ, Güzel M, Açıcı M, Umur Ş, 2016: Molecular survey of Hepatozoon canis in dogs from Samsun Province of Northern part of Turkey. Etlik Vet Mikrobiyol Derg, 27, 104-107.
- De Almeida ME, Koru O, Steurer F, Herwaldt BL, da Silva AJ, 2017: Detection and differentiation of Leishmania spp. in clinical specimens by use of a SYBR Green-Based Real-Time PCR assay. J Clin Microbiol, 55, 281-290.
- De Tommasi AS, Otranto D, Dantas-Torres F, Capelli G, Breitschwerdt EB, de Caprariis D, 2013: Are vector borne pathogen co-infections complicating the clinical presentation in dogs? Parasites & Vectors, 6, 97.
- Diaz MH, Bai Y, Malania L, Winchell JM, Kosoy MY, 2012: Development of a novel genus-specific real-time PCR assay for detection and differentiation of Bartonella species and genotypes. J Clin Microbiol. 50, 1645–1649.
- Duscher GG, Leschnik M, Fuehrer HP, Joachim A, 2014: Wildlife reservoirs for vector-borne canine, feline and zoonotic infections in Austria. Int J Parasitol Parasites Wildl, 4, 88-96.
- Düzlü Ö, İnci A, Yıldırım A, Önder Z, Çiloğlu A, 2014: The investigation of some tick-borne protozoon and rickettsial infections in dogs by Real Time PCR and the molecular characterizations of the detected isolates. Ankara Üniv Vet Fak Derg, 61, 275-282.
- Fang LQ, Liu K, Li XL, Liang S, Yang Y, Yao HW, Sun RX, Sun Y, Chen WJ, Zuo SQ, Ma MJ, Li H, Jiang JF, Liu W, Yang XF, Gray GC, Krause PJ, Cao WC, 2015: Emerging tick-borne infections in mainland China: an increasing public health threat. Lancet Infect Dis, 15, 1467-1479.
- Fouque F, Reeder JC, 2019: Impact of past and on-going changes on climate and weather on vector-borne diseases transmission: a look at the evidence. Infect Dis Poverty, 8, 51.
- Gomes PV, Mundim MJS, Mundim AV, Ávila DF, Guimarães EC, Cury MC, 2010: Occurrence of Hepatozoon sp. In dogs in the urban area originating from a municipality in southeastern Brazil Vet Parasitol, 174, 155–161.
- Guo H, Sevinç F, Ceylan O, Sevinç M, İnce E, Gao Y, Moumouni PFA, Liu M, Efstratiou A, Wang G, Cao S, Zhou M, Jirapattharasate C, Ringo AE, Zheng W, Xuan X, 2017: A PCR survey of vector-borne pathogens in different dog populations from Turkey. Acta Parasitologica, 62, 533–540.
- Güven E, Avcıoğlu H, Cengiz S, Hayırlı A, 2017: Vector borne pathogens in stray dogs in Northeastern Turkey. Vector Borne Zoonotic Dis, 17, 610-617.
- Hall TA, 1999: BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acid Symp, 47, 95- 98.
- Hasegawa M, Kishino H, Yano T, 1985: Dating the human ape split by a molecular clock of mitochondrial DNA. J Mol Evol, 22, 160-174. Jaton K, Peter O, Raoult D, Tissot JD, Greub G, 2013: Development of a high throughput PCR to detect Coxiella burnetii and its application in a diagnostic laboratory over a 7-year period. New Microbes New Infect, 1, 6–12.
- Karagenç T, Hoşgör M, Bilgiç HB, Paşa S, Kirli G, Eren H, 2005: The determination of the prevalence of E. canis, A. phagocytophila, A. platys with nested PCR in dogs in the Aegean Region. İzmir, Turkey: 14th National Parasitology Congress. p. 18–25.
- Kato CY, Chung IH, Robinson LK, Austin AL, Dasch GA, Massung RF, 2013: Assessment of Real-Time PCR assay for detection of Rickettsia spp. and Rickettsia rickettsii in banked clinical samples. J Clin Microbiol, 51, 314–317.
- Kordick SK, Breitschwerdt EB, Hegarty BC, Southwick KL, Colitz CM, Hancock SI, Bradley JM, Rumbough R, Mcpherson JT, MacCormack JN, 1999: Coinfection with multiple tick-borne pathogens in a Walker Hound kennel in North Carolina. J Clin Microbiol, 37, 2631-2638.
- Kumar S, Stecher G, Li M, Knyaz C, Tamura K, 2018: MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol, 35, 1547-1549.
- Little SE, Allen KE, Johnson EM, Panciera RJ, Reichard MV, Ewing SA, 2009: New developments in canine hepatozoonosis in North America: a review. Parasites & Vectors, Suppl 1, S5.
- Maggi RG, Krämer F, 2019: A review on the occurrence of companion vector-borne diseases in pet animals in Latin America. Review. Parasites & Vectors, 12, 145.
- Orkun Ö, Koç N, Nalbantoğlu S, Sürsal N, Çakmak A, Karaer Z, 2018: Molecular Characterization of tick borne blood protozoa in stray dogs from Central Anatolia Region of Turkey with a high-rate hepatozoon infection. Kafkas Univ Vet Fak Derg, 24, 227-232.
- Otranto, Dantas-Torres F, Breitschwerdt EB, 2009a: Managing canine vector-borne diseases of zoonotic concern: part one. Trends Parasitol. 25, 157–163.
- Otranto D, Dantas-Torres F, Breitschwerdt EB, 2009: Managing canine vector-borne diseases of zoonotic concern: Part two. Trends Parasitol, 25, 228–235.
- Otranto D, Dantas-Torres F, Weigl S, Latrofa MS, Stanneck D, de Caprariis D, Capelli G, Baneth G, 2011: Diagnosis of Hepatozoon canis in young dogs by cytology and PCR. Parasites & Vectors, 4, 55.
- Perez M, Bodor M, Zhang C, Xiong Q, Rikihisa Y, 2006: Human infection with Ehrlichia canis accompanied by clinical signs in Venezuela. Ann N Y Acad Sci, 1078, 110-117.
- Perez M, Rikihisa Y, Wen B, 1996: Ehrlichia canis-like agent isolated from a man in Venezuela: antigenic and genetic characterization. J Clin Microbiol, 34, 2133–2139.
- Rishniw M, Barr SC, Simpson KW, Frongillo MF, Franz M, Dominguez Alpizar, JL, 2006: Discrimination between six species of canine microfilariae by a single polymerase chain reaction. Vet Parasitol, 135, 303-314.
- Self SCW, Liu Y, Nordone SK, Yabsley MJ, Walden HS, Lund RB, Dwight D, Bowman D, Carpenter C, McMahan CS, Gettings JR, 2019: Canine vector-borne disease: mapping and the accuracy of forecasting using big data from the veterinary community. Anim Health Res Rev, 20, 47-60.
- Semenza JC, Menne B, 2009: Climate change and infectious diseases in Europe. Lancet Infect Dis, 9, 365–375.
- Tamura K, 1992: Estimation of the number of nucleotide substitutions when there are strong transition transversion and G+C-content biases. Mol Biol Evol, 9, 678-687.
- Versage JL, Severin DD, Chu MC, Petersen JM, 2003: Development of a multitarget real-time TaqMan PCR assay for inhanced detection of Francisella tularensis in complex specimens. J Clin Microbiol, 41, 5492–5499.
- Yu P, Liu Z, Niu Q, Yang J, Abdallah MO, Chen Z, Yin H, 2017: Molecular evidence of tick-borne pathogens in Hyalomma anatolicum ticks infesting cattle in Xinjiang Uygur autonomous region, northwestern China. Exp Appl Acarol, 73 (2), 269–281.
| APA | ASLANTAŞ Ö, Çelebi B, Usluca S (2020). Investigation of Vector-Borne Diseases in Dogs. , 154 - 160. 10.31196/huvfd.792147 |
| Chicago | ASLANTAŞ Özkan,Çelebi Bekir,Usluca Selma Investigation of Vector-Borne Diseases in Dogs. (2020): 154 - 160. 10.31196/huvfd.792147 |
| MLA | ASLANTAŞ Özkan,Çelebi Bekir,Usluca Selma Investigation of Vector-Borne Diseases in Dogs. , 2020, ss.154 - 160. 10.31196/huvfd.792147 |
| AMA | ASLANTAŞ Ö,Çelebi B,Usluca S Investigation of Vector-Borne Diseases in Dogs. . 2020; 154 - 160. 10.31196/huvfd.792147 |
| Vancouver | ASLANTAŞ Ö,Çelebi B,Usluca S Investigation of Vector-Borne Diseases in Dogs. . 2020; 154 - 160. 10.31196/huvfd.792147 |
| IEEE | ASLANTAŞ Ö,Çelebi B,Usluca S "Investigation of Vector-Borne Diseases in Dogs." , ss.154 - 160, 2020. 10.31196/huvfd.792147 |
| ISNAD | ASLANTAŞ, Özkan vd. "Investigation of Vector-Borne Diseases in Dogs". (2020), 154-160. https://doi.org/10.31196/huvfd.792147 |
| APA | ASLANTAŞ Ö, Çelebi B, Usluca S (2020). Investigation of Vector-Borne Diseases in Dogs. Harran Üniversitesi Veteriner Fakültesi Dergisi, 9(2), 154 - 160. 10.31196/huvfd.792147 |
| Chicago | ASLANTAŞ Özkan,Çelebi Bekir,Usluca Selma Investigation of Vector-Borne Diseases in Dogs. Harran Üniversitesi Veteriner Fakültesi Dergisi 9, no.2 (2020): 154 - 160. 10.31196/huvfd.792147 |
| MLA | ASLANTAŞ Özkan,Çelebi Bekir,Usluca Selma Investigation of Vector-Borne Diseases in Dogs. Harran Üniversitesi Veteriner Fakültesi Dergisi, vol.9, no.2, 2020, ss.154 - 160. 10.31196/huvfd.792147 |
| AMA | ASLANTAŞ Ö,Çelebi B,Usluca S Investigation of Vector-Borne Diseases in Dogs. Harran Üniversitesi Veteriner Fakültesi Dergisi. 2020; 9(2): 154 - 160. 10.31196/huvfd.792147 |
| Vancouver | ASLANTAŞ Ö,Çelebi B,Usluca S Investigation of Vector-Borne Diseases in Dogs. Harran Üniversitesi Veteriner Fakültesi Dergisi. 2020; 9(2): 154 - 160. 10.31196/huvfd.792147 |
| IEEE | ASLANTAŞ Ö,Çelebi B,Usluca S "Investigation of Vector-Borne Diseases in Dogs." Harran Üniversitesi Veteriner Fakültesi Dergisi, 9, ss.154 - 160, 2020. 10.31196/huvfd.792147 |
| ISNAD | ASLANTAŞ, Özkan vd. "Investigation of Vector-Borne Diseases in Dogs". Harran Üniversitesi Veteriner Fakültesi Dergisi 9/2 (2020), 154-160. https://doi.org/10.31196/huvfd.792147 |
