The aim of this study is to develop a scale compatible with current animal ethics studies to measure the phenomenon of speciesism, that is marginalization of animals and prejudice and discrimination against animals. In order to develop the Ambivalent Speciesism Scale, an item pool was created by examiningthe animal ethics literature and social psychology studies on human-animal relations, and then the items were edited by taking the opinions of people studying animal rights and experts in measurement and evaluation in psychology. The scale is designed in 7-point Likert type.The trial form was applied to the participants together with the Speciesism Scale, the Social Dominance Orientation Scale and the Basic Empathy Scale. Participants were selected from individuals representing different lifestyles in thecontext of animal use, using the snowball sampling technique. The study was conducted with 288 participants; 64 men, 217 women and, 7 of whom are not of both genders. While there were 24 items in the trial form of the scale, nine of these items were eliminated as a result ofthe factor analysis. The final form of the scalewith 15 items has a high reliability (.90). The items of the scale are divided into three dimensions: belief in human superiority, protective speciesism, and speciesism in language. It was determined that the scores obtained from the scale were in positive correlation with the scores obtained from the other scale measuring speciesism and the social dominance orientation scale, as expected. The scores obtained from the scale are distributed as expected among the groups that include lifestyles related to animal use. These data were evaluated as findings showing the validity of the scale.