Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae)

Gulcin, ilhami; bingöl, zeynebe; DELİMUSTAFAOĞLU BOSTANLIK, Fatmagül; Kılıç, Ceyda Sibel; DUMAN, HAYRI; KARAKAYA, Songül

doi:10.14235/bas.galenos.2020.4127

Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae)

Songül KARAKAYA, (Atatürk University Faculty of Pharmacy, Erzurum, Turkey)

Zeynebe BİNGÖL, (Gaziosmanpaşa University, Vocational School of Health Services, Tokat, Turkey)

İlhami GÜLÇİN, (Atatürk University Faculty of Science, Erzurum, Turkey)

Fatmagül DELİMUSTAFAOĞLU BOSTANLIK, (Ankara University Faculty of Pharmacy, Ankara, Turkey)

Hayri DUMAN, (Gazi University Faculty of Science, Ankara, Turkey)

Ceyda Sibel KILIÇ (Ankara University Faculty of Pharmacy, Ankara, Turkey)

Bezmiâlem Science

7 0

Yıl: 2021 Cilt: 9 Sayı: 2 Sayfa Aralığı: 153 - 157 Metin Dili: İngilizce DOI: 10.14235/bas.galenos.2020.4127 İndeks Tarihi: 14-06-2021

Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae)

Öz:

Objective: Members of the Apiaceae family have many biological andpharmacological effects like anticancer, antidiabetic, antioxidant,anti-inflammatory, anti-hypertensive, anticoagulant, antimicrobial,and anticholinesterase. Also, some of them are consumed asvegetables, pickles, and spices. Carbonic anhydrase has a remarkablerole in diseases like osteoporosis, glaucoma, edema, cancer, epilepsy,and obesity. The inhibition effects of methanol extracts and subextracts (n-hexane, dichloromethane, ethyl acetate, n-butanol, andaqueous residue) of Leiotulus dasyanthus and Ferulago pauciradiataroots on carbonic anhydrase I and II isoenzymes were investigatedin this research.Methods: Leiotulus dasyanthus and Ferulago pauciradiata werecollected in 2017 from Erzurum and in 2013 from Nevşehir(Turkey), respectively. The roots of L. dasyanthus and F. pauciradiatawere macerated with methanol, dispersed with methanol: distilledwater (1:9), and fractionated with n-hexane, dichloromethane, ethylacetate, and n-butanol, in turn. The hCA I and II isoenzymes were isolated from erythrocytes of fresh human blood via Sepharose-4BL-Tyrosine-sulfanilamide affinity chromatography. The analysis ofhCA I and II isoenzymes was actualized by the Verpoorte method.Results: The hCA I and II were influentially inhibited by thedichloromethane and ethyl acetate sub-extracts of F. pauciradiataroot with IC50 values of 1.694 μg/mL towards hCA I and 1.677 μg/mL towards hCA II, respectively. Whereas dichloromethane subextract and methanol extract of L. dasyanthus root inhibited hCA Iand II isoenzymes with IC50 values of 1.828 μg/mL towards hCA Iand 1.852 μg/mL towards hCA II, respectively.Conclusion: The hCA I and II were influentially inhibited bythe dichloromethane and ethyl acetate sub-extracts of Ferulagopauciradiata root.

Anahtar Kelime:

Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. ve Ferulago pauciradiata Boiss.&Heldr.’nın (Apiaceae) Karbonikanhidraz İzoenzimleri I ve II İnhibisyon Potansiyelleri

Öz:

Amaç: Apiaceae familyasının üyeleri antikanser, antidiyabetik, antioksidan, anti-enflamatuvar, antihipertansif, antikoagülan, antimikrobiyal ve antikolinesteraz gibi pek çok biyolojik ve farmakolojik etkiye sahiptir. Ayrıca, bu üyelerin bazıları sebze, turşu ve baharat olarak tüketilmektedir. Karbonik anhidraz osteoporoz, glokom, ödem, kanser, epilepsi ve obezite gibi hastalıklarda önemli bir role sahiptir. Bu çalışmada Leiotulus dasyanthus ve Ferulago pauciradiata köklerinden hazırlanan metanol ekstreleri ve alt ekstrelerinin (n-hekzan, diklorometan, etil asetat, n-butanol ve sulu artık) karbonik anhidraz I ve II izoenzimleri üzerindeki inhibisyon etkileri araştırılmıştır. Yöntemler: Leiotulus dasyanthus ve Ferulago pauciradiata sırasıyla 2017 yılında Erzurum'dan ve 2013'te Nevşehir’den (Türkiye) toplanmıştır. Bitkilerin kökleri metanol ile masere edildi, metanol:su (1:9) ile çözüldükten sonra sırayla n-hekzan, diklorometan, etil asetat ve n-butanol ile fraksiyonlanmıştır. hCA I ve II izoenzimleri, taze insan kanının eritrositlerinden Sepharose-4B-L-Tirozin sülfanilamid afinite kromatografisi yoluyla izole edilmiştir. hCA I ve II izoenzimlerinin analizi Verpoorte metodu ile gerçekleştirilmiştir. Bulgular: hCA I ve II, F. pauciradiata’nın köklerinden hazırlanan diklorometan ve etil asetat alt ekstreleri tarafından IC50 değerleri hCA I’e karşı 1.694 μg/mL ve hCA II’ye karşı ise 1.677 μg/ mL ile etkili bir şekilde inhibe edildi. L. dasyanthus köklerinden hazırlanan diklorometan alt ekstresi ve metanol ekstresi hCA I ve II izoenzimlerini hCA I’e karşı 1.828 μg/mL ve hCA II’ye karşı 1.852 μg/mL IC50 değerleriyle inhibe etmiştir. Sonuç: hCA I ve II, F. pauciradiata’nın köklerinden hazırlanan diklorometan ve etil asetat alt ekstreleri tarafından etkili bir şekilde inhibe edilmiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARAKAYA S, bingöl z, Gulcin i, DELİMUSTAFAOĞLU BOSTANLIK F, DUMAN H, Kılıç C (2021). Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae). , 153 - 157. 10.14235/bas.galenos.2020.4127
Chicago	KARAKAYA Songül,bingöl zeynebe,Gulcin ilhami,DELİMUSTAFAOĞLU BOSTANLIK Fatmagül,DUMAN HAYRI,Kılıç Ceyda Sibel Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae). (2021): 153 - 157. 10.14235/bas.galenos.2020.4127
MLA	KARAKAYA Songül,bingöl zeynebe,Gulcin ilhami,DELİMUSTAFAOĞLU BOSTANLIK Fatmagül,DUMAN HAYRI,Kılıç Ceyda Sibel Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae). , 2021, ss.153 - 157. 10.14235/bas.galenos.2020.4127
AMA	KARAKAYA S,bingöl z,Gulcin i,DELİMUSTAFAOĞLU BOSTANLIK F,DUMAN H,Kılıç C Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae). . 2021; 153 - 157. 10.14235/bas.galenos.2020.4127
Vancouver	KARAKAYA S,bingöl z,Gulcin i,DELİMUSTAFAOĞLU BOSTANLIK F,DUMAN H,Kılıç C Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae). . 2021; 153 - 157. 10.14235/bas.galenos.2020.4127
IEEE	KARAKAYA S,bingöl z,Gulcin i,DELİMUSTAFAOĞLU BOSTANLIK F,DUMAN H,Kılıç C "Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae)." , ss.153 - 157, 2021. 10.14235/bas.galenos.2020.4127
ISNAD	KARAKAYA, Songül vd. "Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae)". (2021), 153-157. https://doi.org/10.14235/bas.galenos.2020.4127

APA	KARAKAYA S, bingöl z, Gulcin i, DELİMUSTAFAOĞLU BOSTANLIK F, DUMAN H, Kılıç C (2021). Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae). Bezmiâlem Science, 9(2), 153 - 157. 10.14235/bas.galenos.2020.4127
Chicago	KARAKAYA Songül,bingöl zeynebe,Gulcin ilhami,DELİMUSTAFAOĞLU BOSTANLIK Fatmagül,DUMAN HAYRI,Kılıç Ceyda Sibel Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae). Bezmiâlem Science 9, no.2 (2021): 153 - 157. 10.14235/bas.galenos.2020.4127
MLA	KARAKAYA Songül,bingöl zeynebe,Gulcin ilhami,DELİMUSTAFAOĞLU BOSTANLIK Fatmagül,DUMAN HAYRI,Kılıç Ceyda Sibel Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae). Bezmiâlem Science, vol.9, no.2, 2021, ss.153 - 157. 10.14235/bas.galenos.2020.4127
AMA	KARAKAYA S,bingöl z,Gulcin i,DELİMUSTAFAOĞLU BOSTANLIK F,DUMAN H,Kılıç C Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae). Bezmiâlem Science. 2021; 9(2): 153 - 157. 10.14235/bas.galenos.2020.4127
Vancouver	KARAKAYA S,bingöl z,Gulcin i,DELİMUSTAFAOĞLU BOSTANLIK F,DUMAN H,Kılıç C Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae). Bezmiâlem Science. 2021; 9(2): 153 - 157. 10.14235/bas.galenos.2020.4127
IEEE	KARAKAYA S,bingöl z,Gulcin i,DELİMUSTAFAOĞLU BOSTANLIK F,DUMAN H,Kılıç C "Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae)." Bezmiâlem Science, 9, ss.153 - 157, 2021. 10.14235/bas.galenos.2020.4127
ISNAD	KARAKAYA, Songül vd. "Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae)". Bezmiâlem Science 9/2 (2021), 153-157. https://doi.org/10.14235/bas.galenos.2020.4127