Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity
Yıl: 2020 Cilt: 4 Sayı: 1 Sayfa Aralığı: 1 - 7 Metin Dili: İngilizce DOI: 10.30616/ajb.690005 İndeks Tarihi: 14-06-2021
Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity
Öz: Armillaria mellea (Vahl) P. Kumm. commonly-known as the honey fungus is an edible mushroom and its antioxidant,antitumor, neuroprotective, and insulin resistance reductive effects have been well-characterized. Glutathione S-transferases(GST) are the group of detoxification enzymes has a function to conjugate glutathione to the variety of electrophile, makingthem more water-soluble for excretion. Their inhibition or activation could have profound toxicological or clinical implications.This study is conducted to investigate the inhibitory effects of A. mellea on GST enzymes. Total GST activities were measuredusing glutathione and 1 chloro-2,4-dinitrobenzene as substrates spectrophotometrically, and enzyme kinetic studies wereconducted to determine Vmax and Km values. Additionally, aqueous and methanolic extracts of A. mellea were tested to see howthey modulate the kinetic parameters. Vmax for liver GST enzyme was calculated as 443.90 ± 11.52 U/mg, Km value for GSH,and CDNB were determined as 4.88 ± 0.53 mM and 10.43 ± 1.07 mM, respectively. The decrease in Vmax and the increase in Kmvalues with A. mellea extracts demonstrated a mixed-type inhibitory mechanism. Methanolic extract inhibits the GSH-dependentGST activity much more than the CDNB-dependent activity, but aqueous extract mainly affects CDNB-dependent GST activity.In conclusion, A. mellea could suppress the hepatic GST enzymes that might have toxicological consequences such as reducedcellular detoxification of electrophilic xenobiotics as well as alleviated drug resistance in the treatment of several diseases.
Anahtar Kelime: Armillaria mellea (Vahl) P. Kumm’nın karaciğer glutatyon S-transferaz aktivitesi üzerine baskılayıcı etkileri
Öz: Armillaria mellea (Vahl) P. Kumm. yaygın olarak bal mantarı olarak bilinen yenilebilir bir mantardır ve antioksidan, antitümör, nöroprotektif ve insülin direncini azaltıcı etkileri iyi karakterize edilmiştir. Glutatyon S-transferazlar (GST), detoksifikasyon enzimlerinin bir grubudur ve glutatyonu çeşitli elektrofillere konjüge etme fonksiyonuna sahiptir. Bu işlem onları atılım için suda daha fazla çözünür hale getirir. GST enzimlerinin baskılanması veya aktive edilmesinin toksikolojik veya klinik etkileri olabilir. Bu çalışma A. mellea'nın GST'ler üzerindeki baskılayıcı etkilerini araştırmak için yapılmıştır. Toplam GST aktiviteleri, spektrofotometrik olarak glutatyon ve 1 kloro-2,4-dinitrobenzen substratları kullanılarak ölçülmüş, Vmax ve Km değerlerini belirlemek için enzim kinetiği çalışmaları yapılmıştır. Ek olarak, A. mellea'nın sulu ve metanolik özütleri, kinetik parametreleri nasıl modüle ettiklerini değerlendirmek için test edilmiştir. Karaciğer GST enzimi için Vmax 443,90±11,52 U/mg, GSH ve CDNB için Km değerleri sırasıyla 4,88±0,53 mM ve 10,43±1,07 mM olarak belirlenmiştir. A. mellea ekstreleri ile Vmax'daki azalma ve Km değerlerindeki artış, GST enziminin karışık tipte inhibisyon mekanizmasıyla baskılandığını göstermiştir. Metanolik ekstraktın GSH-bağımlı GST aktivitesini CDNB-bağımlı aktiviteden çok daha fazla inhibe ettiği, fakat su ekstraktlarının esas olarak CDNB-bağımlı GST aktivitesini baskıladığı gösterilmiştir. Sonuç olarak A. mellea’nın, karaciğer GST enzimini baskılayarak ksenobiyotiklerin hücresel detoksifikasyonunu azaltabilecek ve çeşitli hastalıkların tedavisinde ilaç direncinin azaltılması gibi toksikolojik sonuçları olabilecek bir mantar türü olabileceği değerlendirilmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | KÜTÜK A, Sadi G (2020). Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity. , 1 - 7. 10.30616/ajb.690005 |
| Chicago | KÜTÜK AYŞEGÜL,Sadi Gökhan Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity. (2020): 1 - 7. 10.30616/ajb.690005 |
| MLA | KÜTÜK AYŞEGÜL,Sadi Gökhan Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity. , 2020, ss.1 - 7. 10.30616/ajb.690005 |
| AMA | KÜTÜK A,Sadi G Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity. . 2020; 1 - 7. 10.30616/ajb.690005 |
| Vancouver | KÜTÜK A,Sadi G Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity. . 2020; 1 - 7. 10.30616/ajb.690005 |
| IEEE | KÜTÜK A,Sadi G "Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity." , ss.1 - 7, 2020. 10.30616/ajb.690005 |
| ISNAD | KÜTÜK, AYŞEGÜL - Sadi, Gökhan. "Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity". (2020), 1-7. https://doi.org/10.30616/ajb.690005 |
| APA | KÜTÜK A, Sadi G (2020). Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity. Anatolian Journal of Botany, 4(1), 1 - 7. 10.30616/ajb.690005 |
| Chicago | KÜTÜK AYŞEGÜL,Sadi Gökhan Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity. Anatolian Journal of Botany 4, no.1 (2020): 1 - 7. 10.30616/ajb.690005 |
| MLA | KÜTÜK AYŞEGÜL,Sadi Gökhan Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity. Anatolian Journal of Botany, vol.4, no.1, 2020, ss.1 - 7. 10.30616/ajb.690005 |
| AMA | KÜTÜK A,Sadi G Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity. Anatolian Journal of Botany. 2020; 4(1): 1 - 7. 10.30616/ajb.690005 |
| Vancouver | KÜTÜK A,Sadi G Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity. Anatolian Journal of Botany. 2020; 4(1): 1 - 7. 10.30616/ajb.690005 |
| IEEE | KÜTÜK A,Sadi G "Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity." Anatolian Journal of Botany, 4, ss.1 - 7, 2020. 10.30616/ajb.690005 |
| ISNAD | KÜTÜK, AYŞEGÜL - Sadi, Gökhan. "Inhibitory effects of Armillaria mellea (Vahl) P. Kumm. on liver glutathione S-transferase activity". Anatolian Journal of Botany 4/1 (2020), 1-7. https://doi.org/10.30616/ajb.690005 |
