Objective: To evaluate the awareness of nurses about evidence-based nursing (EBN) practices and the effects of the training of EBN practices on given to nurses on their attitudes towards EBN. Materials and Methods: This is a descriptive and semi-experimental study conducted in a Training and Research Hospital in Ankara between February and May 2018. The sample of the nurses (n=105) who participated in the study were included in the training on EBN practices in the in-service training plan of the hospital. The data collection form consists of 4 parts. In the first part, socio-demographic characteristics, in the second part awareness assessment questions about EBN practices, in the third section the attitude towards EBN Questionnaire (pre-training) and in the fourth part the this questionnaire (post-training). Wilcoxoen Signed Rank Test, Mann-Whitney U Test and Kruskal Wallis Test were used to evaluate the data. For statistical significance p <0.05 was accepted. Results: The mean age of the nurses participating in the study was 37.24±5.75 years, the study year was 15.63±6.79 years, 34.3% of them were bachelor's degree, 59% did not know the definition of EBN. Significant differences were found between nurses' scores of Atitude Towards EBN Questionnaire before and after training (p=0.000). Conclusion: In this study, it was found that nurses' scores of atitude EBN Questionnaire were at a moderate level and after the training on EBN, the total score of Attitude Towards EBN Questionnaire was increased.