Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model

KIRDAN, Taylan; YILMAZ, Enis ÇAĞATAY; KUMANDAŞ, ALİ; EKİCİ, Hüsamettin; GÜZEL, Şevket Ergun; Emre Aydıngöz, Selda; GEDAR, Özgün Melike TOTUK; Midi, Ahmet

doi:10.14744/tjtes.2020.50384

Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model

Özgün Melike TOTUK GEDAR, (Bahçeşehir Üniversitesi, Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, İstanbul, Türkiye)

Şevket Ergun GÜZEL, (Sağlık Bakanlığı Adıyaman Kahta Devlet Hastanesi, Ortopedi ve Travmatoloji Kliniği, Adıyaman, Türkiye)

Hüsamettin EKİCİ, (Kırıkkale Üniversitesi, Veterinerlik Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dalı, Kırıkkale, Türkiye)

Ali KUMANDAŞ, (Kırıkkale Üniversitesi, Veterinerlik Fakültesi, Cerrahi Anabilim Dalı, Kırıkkale, Türkiye)

Selda EMRE AYDINGÖZ, (Başkent Üniversitesi, Tıp Fakültesi, Tıbbi Farmakoloji Anabilim Dalı, Ankara, Türkiye)

Enis ÇAĞATAY YILMAZ, (Bahçeşehir Üniversitesi, Tıp Fakültesi, İstanbul, Türkiye)

Taylan KIRDAN, (Bahçeşehir Üniversitesi, Tıp Fakültesi, İstanbul, Türkiye)

Ahmet MİDİ (Bahçeşehir Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, İstanbul, Türkiye)

Ulusal Travma ve Acil Cerrahi Dergisi

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Yıl: 2020 Cilt: 26 Sayı: 6 Sayfa Aralığı: 853 - 858 Metin Dili: İngilizce DOI: 10.14744/tjtes.2020.50384 İndeks Tarihi: 18-06-2021

Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model

Öz:

BACKGROUND: Algan Hemostatic Agent (AHA) is a multi-herbal extract containing a standardized amount of Achillea millefolium, Juglans regia, Lycopodium clavatum, Rubus caesius or Rubis fruciosus, Viscum album, and Vitis vinifera, each of which is effective in hemostasis. In this study, we aimed to investigate the effects of AHA on bleeding time in a rat tail hemorrhage model.METHODS: Forty-eight Sprague Dawley rats (5–7 weeks old, 180–210 g) were randomly and equally allocated to six groups as follows: heparin plus saline (heparinized control), heparin plus AHA-soaked sponge, heparin plus liquid form of AHA, saline (non-heparinized control), AHA-soaked sponge and liquid form of AHA. Heparin (640 IU/kg) was administered intraperitoneally three times a day for three days in heparinized groups. For the bleeding model, the tail of rats was transected. According to the study group, either saline- or AHA-soaked sponge or liquid form of AHA was applied over the hemorrhage area. In AHA- or saline-soaked sponge groups, once the bleeding time had started, it was checked every 10 seconds. If the bleeding did not stop after 40 seconds, it was accepted as a failure. In liquid AHA group, the duration of bleeding was measured using a chronometer and defined as the time (seconds) from wounding until the bleeding stopped.RESULTS: Bleeding time in the heparinized and non-heparinized control groups was over 40 seconds. After applying the sponge form of AHA on the wound area, bleeding time was significantly shortened to less than 20 seconds in both heparinized and non-heparinized rats (p<0.001 for both). The liquid form of AHA stopped bleeding in 5.0±1.2 seconds and 8.0±1.3 seconds in heparinized and non-heparinized groups, respectively.CONCLUSION: AHA is a highly effective topical hemostatic agent in a rat tail hemorrhage model, thus may provide for a unique clinically effective option for control of bleeding during surgical operations or other emergencies.

Anahtar Kelime:

Algan hemostatik ajan’ın sıçan kuyruk kanama modelinde kanama zamanı üzerine etkisi

Öz:

AMAÇ: Algan hemostatik ajan (AHA), hemostatik etkinliği bilinen Achillea millefolium, Juglans regia, Lycopodium clavatum, Rubus caesius ya da Rubis fruciosus, Viscum album ve Vitis viniferea’yı standardize miktarda içeren bir bitki ekstresidir. Bu çalışmada AHA’nın sıçan kuyruk kanama modelinde kanama zamanı üzerine etkinliğinin değerlendirilmesi amaçlanmıştır.GEREÇ VE YÖNTEM: Kırk sekiz Sprague Dawley sıçan (5–7 haftalık, 180–210 g), altı çalışma grubuna rastgele ve eşit sayıda randomize edildi. Çalışma grupları şunlardır: Heparin + salin (heparinize kontrol), heparin + AHA ile ıslatılmış sünger, heparin + sıvı AHA, salin (heparinize olmayan kontrol), AHA ile ıslatılmış sünger, sıvı AHA. Heparinize gruptaki sıçanlara üç gün boyunca günde üç kez intraperitoneal heparin (640 IU/kg) uygu-landı. Kanama modeli oluşturmak için sıçanların kuyrukları kesildi. Çalışma grubuna göre kanama bölgesine salin ile ıslatılmış sünger, AHA ile ıslatılmış sünger ya da sıvı AHA uygulandı. Salin ya da AHA ile ıslatılmış sünger uygulanan gruplarda, kanamanın durumu her 10 saniyede bir kontrol edildi. Kanama 40 saniye sonra hala durmamış ise uygulanan tedavi başarısız kabul edildi. Sıvı AHA uygulanan grupta, kanama süresi kuyruk kesilmesinden kanama durana kadar geçen süre olarak tanımlandı ve kronometre ile ölçüldü.BULGULAR: Heparin uygulanan ve uygulanmayan kontrol gruplarında kanama süresi 40 saniyenin üzerinde kaydedildi. Kanama bölgesine AHA ile ıslatılmış sünger uygulanan heparinize olan ve olmayan sıçanlarda ise kanama süresi anlamlı olarak kısalarak 20 saniyenin altına düştü (her iki grup için de p<0.001). Sıvı AHA uygulanan heparinize olan ve olmayan sıçanlarda kanama süresi sırasıyla 5.0±1.2 saniye ve 8.0±1.3 saniye olarak ölçüldü.TARTIŞMA: AHA, sıçan kuyruk kanama msodelinde yüksek etkinliğe sahip bir hemostatik ajandır. Cerrahi girişimlerde ve acil durumlarda kanama kontrolü sağlanması için kullanılabilecek bir tedavi seçeneği olarak değerlendirilebilir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	GEDAR Ö, GÜZEL Ş, EKİCİ H, KUMANDAŞ A, Emre Aydıngöz S, YILMAZ E, KIRDAN T, Midi A (2020). Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model. , 853 - 858. 10.14744/tjtes.2020.50384
Chicago	GEDAR Özgün Melike TOTUK,GÜZEL Şevket Ergun,EKİCİ Hüsamettin,KUMANDAŞ ALİ,Emre Aydıngöz Selda,YILMAZ Enis ÇAĞATAY,KIRDAN Taylan,Midi Ahmet Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model. (2020): 853 - 858. 10.14744/tjtes.2020.50384
MLA	GEDAR Özgün Melike TOTUK,GÜZEL Şevket Ergun,EKİCİ Hüsamettin,KUMANDAŞ ALİ,Emre Aydıngöz Selda,YILMAZ Enis ÇAĞATAY,KIRDAN Taylan,Midi Ahmet Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model. , 2020, ss.853 - 858. 10.14744/tjtes.2020.50384
AMA	GEDAR Ö,GÜZEL Ş,EKİCİ H,KUMANDAŞ A,Emre Aydıngöz S,YILMAZ E,KIRDAN T,Midi A Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model. . 2020; 853 - 858. 10.14744/tjtes.2020.50384
Vancouver	GEDAR Ö,GÜZEL Ş,EKİCİ H,KUMANDAŞ A,Emre Aydıngöz S,YILMAZ E,KIRDAN T,Midi A Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model. . 2020; 853 - 858. 10.14744/tjtes.2020.50384
IEEE	GEDAR Ö,GÜZEL Ş,EKİCİ H,KUMANDAŞ A,Emre Aydıngöz S,YILMAZ E,KIRDAN T,Midi A "Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model." , ss.853 - 858, 2020. 10.14744/tjtes.2020.50384
ISNAD	GEDAR, Özgün Melike TOTUK vd. "Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model". (2020), 853-858. https://doi.org/10.14744/tjtes.2020.50384

APA	GEDAR Ö, GÜZEL Ş, EKİCİ H, KUMANDAŞ A, Emre Aydıngöz S, YILMAZ E, KIRDAN T, Midi A (2020). Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model. Ulusal Travma ve Acil Cerrahi Dergisi, 26(6), 853 - 858. 10.14744/tjtes.2020.50384
Chicago	GEDAR Özgün Melike TOTUK,GÜZEL Şevket Ergun,EKİCİ Hüsamettin,KUMANDAŞ ALİ,Emre Aydıngöz Selda,YILMAZ Enis ÇAĞATAY,KIRDAN Taylan,Midi Ahmet Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model. Ulusal Travma ve Acil Cerrahi Dergisi 26, no.6 (2020): 853 - 858. 10.14744/tjtes.2020.50384
MLA	GEDAR Özgün Melike TOTUK,GÜZEL Şevket Ergun,EKİCİ Hüsamettin,KUMANDAŞ ALİ,Emre Aydıngöz Selda,YILMAZ Enis ÇAĞATAY,KIRDAN Taylan,Midi Ahmet Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model. Ulusal Travma ve Acil Cerrahi Dergisi, vol.26, no.6, 2020, ss.853 - 858. 10.14744/tjtes.2020.50384
AMA	GEDAR Ö,GÜZEL Ş,EKİCİ H,KUMANDAŞ A,Emre Aydıngöz S,YILMAZ E,KIRDAN T,Midi A Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model. Ulusal Travma ve Acil Cerrahi Dergisi. 2020; 26(6): 853 - 858. 10.14744/tjtes.2020.50384
Vancouver	GEDAR Ö,GÜZEL Ş,EKİCİ H,KUMANDAŞ A,Emre Aydıngöz S,YILMAZ E,KIRDAN T,Midi A Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model. Ulusal Travma ve Acil Cerrahi Dergisi. 2020; 26(6): 853 - 858. 10.14744/tjtes.2020.50384
IEEE	GEDAR Ö,GÜZEL Ş,EKİCİ H,KUMANDAŞ A,Emre Aydıngöz S,YILMAZ E,KIRDAN T,Midi A "Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model." Ulusal Travma ve Acil Cerrahi Dergisi, 26, ss.853 - 858, 2020. 10.14744/tjtes.2020.50384
ISNAD	GEDAR, Özgün Melike TOTUK vd. "Effects of Algan Hemostatic Agent on bleedingtime in a rat tail hemorrhage model". Ulusal Travma ve Acil Cerrahi Dergisi 26/6 (2020), 853-858. https://doi.org/10.14744/tjtes.2020.50384