Globally 364102 healthcare professionals have been infected with severe acute respiratory syndromecoronavirus-2 (SARS-CoV-2) and 1253 of them died until 15 January 2021. Healthcare professionalsserving at the forefront of combating the pandemic are in the high risk group. In our country, the dataabout coronavirus-2019 (COVID-19) among healthcare professionals are limited. The aim of this studywas to investigate the anti-SARS-CoV-2 IgG seroprevalence in healthcare professionals, to evaluate therisks they encountered during work, and to examine their relationships with antibody positivity. A totalof 572 healthcare professionals serving in various units of our hospital participated in our study and thepresence of anti-nucleocapsid IgG was investigated by chemiluminescent microparticle immunoassay(SARS-CoV-2 IgG test, Abbott Laboratories Diagnostics, USA) method in serum samples collected betwe en May 18, 2020 and June 30, 2020. The demographic characteristics, medical history, work conditions,medical procedures performed and possible risk factors were questioned with a questionnaire form. Theaverage age of the participants was 33.5 ± 9.2 (19-61) years, and 62.9% (360/572) of them were wo men. In our study, the anti-SARS-CoV-2 IgG seroprevalence was 3.7% (21/572). The association of theantibody positivity with age, gender and occupational status was not statistically significant (p> 0.05).Comorbid diseases which were significantly higher in seropositive healthcare professionals were hyper tension (19%) and diabetes mellitus (14.3%) (p< 0.05). It was observed that antibody positivity wassignificantly higher in healthcare professionals working in high (52.4%) and medium risk (33.3%) areas,those who treat and/or examine patients with suspicious or positive COVID-19 (66.7%) and those who spend more than 30 minutes in COVID-19 patient rooms (76%) (p< 0.05). The symptoms associatedwith seropositivity in healthcare workers with a history of symptoms (46%) were loss of smell (23.5%),loss of taste (20.0%) and respiratory distress (16.7%) (p< 0.05). It was observed that the probability ofbeing infected with SARS-CoV-2 increased 12 times if there was a colleague with COVID-19 in the hos pital, four times if there was a patient in the house/lodging and six times if there was an infected personin the social environment (p< 0.05). The rate of those who had the flu vaccine among the participantswas 10.8% (62/572) and 9.7% of them were found to be anti-SARS-CoV-2 IgG positive (p< 0.05, 95%CI= 1.31-9.48). The seropositivity was significantly higher in non-smokers (4.8 %) compared to smokers(0.0%) (p< 0.05). In our study, it was determined that the rate of seropositivity was 12 times higher inhealthcare professionals who stated that they received hydroxychloroquine prophylaxis due to risky con tact compared to those who did not receive prophylaxis (p< 0.05, 95% CI= 4.11-40.64). The ratio of thepersonnel who answered “always” to the frequency of wearing gloves, masks, goggles/face shields andoveralls was 85.7%, 96.9%, 62.1% and 65.4%, respectively. In conclusion, regular and large-scale sero epidemiological screening of healthcare professionals in the COVID-19 pandemic can contribute to thecontrol of the pandemic by providing a better understanding of transmission dynamics and risk factors.