Background and Aims: This study reveals the anatomical features of Hypericum kotschyanum Boiss. species and compares them with previous studies. The anatomical characteristics of stem, leaf, and root were studied using a light microscope. In addition, anatomical structures were measured. Methods: The studied material was collected from Arslanköy, Mersin. The collected specimens were identified. Dried specimens were kept in Istanbul University Faculty of Pharmacy Herbarium (ISTE) and filed using the ISTE number system (ISTE 98173). Also, some plant materials were kept in 70% ethanol for anatomical examination. All sections from plants were cut by hand using a blade. Samples were examined in SARTUR reagent. Photographs were taken using a light microscope.Results: When we examined the cells around the stomata in the light of the neighboring cells, we observed that the stomata is an anomocytic type in leaf superficial section. The number of cells that radially surround the bottom of the trichomes on the leaf surface is 9. This can be specified as a characteristic feature of this type. In the stem cross-section of H. kotschyanumspecies, secretory canals were observed. In H. kotschyanum stem cross-section, irregular ridges were present and wing structure was not observed. The existence of secretory canals was observed in H. kotschyanum root cross-section. Conclusion: We examined Hypericum kotschyanum anatomically for the first time. Therefore, this study is important in providing information that will be the source of other studies on this plant.