Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması

Amdia MAHAMADU, (Atatürk Üniversitesi, Veteriner Fakültesi, Biyokimya Ana Bilim Dalı, Erzurum, Türkiye)
Fatih Mehmet KANDEMİR (Atatürk Üniversitesi, Veteriner Fakültesi, Biyokimya Ana Bilim Dalı, Erzurum, Türkiye)
Kocatepe Veterinary Journal
23 4
Yıl: 2021 Cilt: 14 Sayı: 3 Sayfa Aralığı: 325 - 338 Metin Dili: Türkçe DOI: 10.30607/kvj.940020 İndeks Tarihi: 29-07-2022

Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması

Öz:
Bu çalışmada, ratlarda sisplatin (SP) kaynaklı hepatotoksisite üzerine zingeron’ un (ZO) etkilerinin araştırılması amaçlandı. Çalışmada 35 adet Sprague Dawley erkek rat kullanıldı. Hayvanlar, her grupta 7 rat olacak şekilde 5 gruba ayrıldı. 1.Grup (Kontrol): Sadece oral serum fizyolojik (SF, 7 gün) verildi. 2. Grup (SP): Tek doz 7 mg/kg SP periton içi (i.p.) uygulandı. 3.Grup (ZO): ZO 7 gün boyunca 50 mg/kg/gün dozunda oral verildi. 4. Grup (SP+ZO 25): Tek doz SP uygulamasından 30 dk sonra 25 mg/kg/gün dozunda ZO verilmeye başlandı ve 7 gün devam etti. 5.Grup (SP+ZO 50): Tek doz SP uygulamasından 30 dk sonra 50 mg/kg/gün dozunda ZO verildi ve 7 gün devam etti. Alanin transaminaz (ALT), aspartat transaminaz (AST) ve alkalen fosfataz (ALP) aktivitelerinin SP uygulaması ile arttığı, antioksidan enzimlerden süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), katalaz (KAT) aktiviteleri ile non-enzimatik antioksidan olan redükte glutatyon (GSH) seviyelerinin SP grubunda düştüğü, malondialdehit (MDA) düzeyinin arttığı görülmüş, ZO’ un ise antioksidan sistemi desteklediği ve MDA düzeylerini azalttığı gözlenmiştir. SP uygulamasının arginaz aktivitesini düşürürken nitrik oksit (NO), 8-hidroksi-2'-deoksiguanozin (8-OHdG), sistein aspartat spesifik proteaz-3 (kaspaz-3), tümör nekroz faktör-alfa (TNF-α), nükleer faktör kappa B (NF-ĸB) ve B-hücreli lenfoma-3 (Bcl-3) seviyelerini artırdığı, ZO’ un bu parametrelerde iyileşme sağladığı saptandı.
Anahtar Kelime: İnflamasyon Zingeron Sisplatin Oksidatif stres Hepatotoksisite

Investigation of the Effect of Zingerone on Some Biochemical Parameters on Cisplatin-Induced Liver Toxicity in Rats

Öz:
This study was aimed to investigate the protective effects of zingerone (ZO) on cisplatin (CP)-induced hepatotoxicity in rats. Thirty-five (35) Sprague Dawley male rats were used in the study; the animals were divided into 5 groups of 7 rats in each group. Group 1 (Control): received only oral serum physiologic (SP, for 7 days). Group 2 (CP): received a single dose of 7 mg/kg intraperitoneal (i.p.) of CP. Group 3 (ZO): Received daily oral doses of ZO at 50 mg/kg/day for 7 days. Group 4 (CP+ZO 25): Oral administration of ZO at 25 mg/kg/day was started 30 minutes after a single dose CP application and continued for 7 days. Group 5 (CP+ZO 50): Oral administration of ZO at 50 mg/kg/day was started 30 minutes after a single dose CP application and continued for 7 days. Alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) activities increased due to CP administration. Also, enzymatic antioxidants; superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH) level were shown to had decreased upon CP administration. After ZO was supported by antioxidant system and decreased malondialdehyde (MDA) levels. It was determined that CP administration increased nitric oxide (NO), 8-hydroxy-2'- deoxyguanosine (8-OHdG), cysteine aspartate specific protease-3 (caspase-3), tumor necrosis factor-alpha (TNF-α), nuclear factor kappa B (NF-κB) and B-cell lymphoma-3 (Bcl-3) levels while arginase activity reduced, ZO administrations could have been the main cause of the improved effects on the parameters.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA MAHAMADU A, KANDEMİR F (2021). Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması. , 325 - 338. 10.30607/kvj.940020
Chicago MAHAMADU Amdia,KANDEMİR Fatih Mehmet Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması. (2021): 325 - 338. 10.30607/kvj.940020
MLA MAHAMADU Amdia,KANDEMİR Fatih Mehmet Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması. , 2021, ss.325 - 338. 10.30607/kvj.940020
AMA MAHAMADU A,KANDEMİR F Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması. . 2021; 325 - 338. 10.30607/kvj.940020
Vancouver MAHAMADU A,KANDEMİR F Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması. . 2021; 325 - 338. 10.30607/kvj.940020
IEEE MAHAMADU A,KANDEMİR F "Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması." , ss.325 - 338, 2021. 10.30607/kvj.940020
ISNAD MAHAMADU, Amdia - KANDEMİR, Fatih Mehmet. "Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması". (2021), 325-338. https://doi.org/10.30607/kvj.940020
APA MAHAMADU A, KANDEMİR F (2021). Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması. Kocatepe Veterinary Journal, 14(3), 325 - 338. 10.30607/kvj.940020
Chicago MAHAMADU Amdia,KANDEMİR Fatih Mehmet Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması. Kocatepe Veterinary Journal 14, no.3 (2021): 325 - 338. 10.30607/kvj.940020
MLA MAHAMADU Amdia,KANDEMİR Fatih Mehmet Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması. Kocatepe Veterinary Journal, vol.14, no.3, 2021, ss.325 - 338. 10.30607/kvj.940020
AMA MAHAMADU A,KANDEMİR F Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması. Kocatepe Veterinary Journal. 2021; 14(3): 325 - 338. 10.30607/kvj.940020
Vancouver MAHAMADU A,KANDEMİR F Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması. Kocatepe Veterinary Journal. 2021; 14(3): 325 - 338. 10.30607/kvj.940020
IEEE MAHAMADU A,KANDEMİR F "Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması." Kocatepe Veterinary Journal, 14, ss.325 - 338, 2021. 10.30607/kvj.940020
ISNAD MAHAMADU, Amdia - KANDEMİR, Fatih Mehmet. "Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması". Kocatepe Veterinary Journal 14/3 (2021), 325-338. https://doi.org/10.30607/kvj.940020
Sayfa Oluşturulma Tarihi
23-04-2024 20:5

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