Background/Aims: Although many regimens, including quadruple, sequential, and concomitant treatment, are used and recommendedas first-line or rescue therapies for Helicobacter pylori infection, eradication rates are still below 90% in intention-to-treat analyses.Treatment protocols with substantially high eradication rates and low antibiotic resistance are needed. In this study, we investigatedthe efficacy of high-dose dual therapy as first-line treatment in a Turkish population.Materials and Methods: All patients underwent upper gastrointestinal endoscopy for the initial H. pylori status because of dyspepticsymptoms. All patients received a 14-day, high-dose dual therapy comprising rabeprazole (20 mg t.i.d.) and amoxicillin (1 g t.i.d.) for H.pylori eradication. H. pylori stool antigen tests of eradication were administered to all participants at least 4 weeks after the completionof the treatment.Results: The high-dose dual therapy demonstrated a 91.3% rate of successful eradication of H. pylori infection. Per-protocol successwas 94.4% among female patients (n=51) and 89.6% among male patients (n=86); in terms of gender, the differences were not significant (p=0.310). No side effects were observed during the study in any patient. Six other patients did not take adequate doses of thetreatment protocol.Conclusion: High-dose dual therapy with rabeprazole and amoxicillin was highly effective and well tolerated as a first-line therapy forH. pylori eradication