Aim: The research was carried out to examine the patient safety cultures of surgical nurses' and the factors affecting this.Methods: The research was carried out descriptively and cross-sectionally. The research population consists of nurses working in the surgical clinics of a university hospital between May and December 2018.It is aimed to reach the entire population (98 nurses) without going through the sample selection. There were 79 (80.6%) nurses in the sample because the nurses who were on leave or were on duty and did not agree to participate in the study were excluded from the study. The data were obtained by applying the Individual Promotion Form and the Patient Safety Culture Scale.Results: It was determined that 81% of the nurses, whose average age was 29.47±6.75, were women. 58.2% of the nurses were bachelor and master degree graduates, and 89.9% were trained in patient safety. It was determined that the total score average taken from the scale was 2.81±0.46. The highest score obtained belongs to the sub-dimensions of “employee training” and “employee behaviour” and the lowest score obtained belongs to the sub-dimension of “management leadership”. According to the professional satisfaction and weekly working hours of the nurses, there was a significant difference between patient safety cultures.Conclusion: The findings can be interpreted that the patient safety culture is moderate in participating nurses. In order to ensure patient safety, a safety culture should be developed in healthcare delivery. For this purpose, in-house training can be recommended. In order to evaluate the patient safety culture of nurses, studies covering larger samples are needed.