OBJECTIVE: Explaining the isolation control methods and giving importance to this issue in the preclinical period will help the doctor candidates to protect themselves and their patients during their risky business lives. The aim of this study is to evaluate the knowledge status, associated factors and training needs of the 3rd grade medical faculty students who were at preclinical period about isolation precaution methods. MATERIAL AND METHODS: In this descriptive study, the questionnaire consisting of the questions which evaluated the general information of the students and the information about the isolation precaution methods was applied to the 3rd grade students of the Faculty of Medicine who wanted to participate in the study between October 2018 and February 2019. RESULTS: 49.3% of the students were women; 50.7% of them were male. It was determined that the students, who were informed about the isolation precautions icons at the hospital (p= 0,045) and who received training on hand hygiene and isolation precautions (p= 0,025) knew the knowledge and practices regarding hand hygiene more accurately than others. Students who had a history of hospitalization (p= 0,014), were informed about the isolation precautions icons at the hospital (p= 0,017) and who received training on hand hygiene and isolation precautions (p= 0,000) knew the knowledge and practices regarding isolation precautions more accurately than others. CONCLUSIONS: In order for knowledge and attitude to become a behaviour, training on issues that have a major place in infection control should be initiated as early as possible and the trainings should be renewed at regular intervals. It is very important that physicians who should be a role model in this subject and who need to protect themselves professionally in the preclinical period.