Effect of epirubicin on mitotic index in cultured L-cells

Topçul, Mehmet Rıfkı; ARICAN, Özcan Gül

Effect of epirubicin on mitotic index in cultured L-cells

Özcan Gül ARICAN, (İstanbul Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, İstanbul, Türkiye)

Mehmet Rıfkı TOPÇUL (İstanbul Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, İstanbul, Türkiye)

Journal of Cell and Molecular Biology

2 1

Yıl: 2003 Cilt: 2 Sayı: 1 Sayfa Aralığı: 43 - 48 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of epirubicin on mitotic index in cultured L-cells

Öz:

Kanser kemoterapisi, yaygın tümörlerin tedavisinde cerrahi uygulama ve radyoterapinin yanında gerçeklefltirilen ek bir uygulamadır. Antrasiklin türevi bir antibiyotik olan epirubisin (EPI) klinik olarak kullanılan antineoplastik ilaçlardan birisidir. Bu çalışmada, EPI in sitotoksik etkileri, transforme edilmiş fare fibroblastlarında (L-hücreleri) araştırıldı. EPI in 0.001 µg/ml, 0.01 µg/ml ve 0.1 µg/ml konsantrasyonları 2, 4, 8, 16 ve 32 saat süresince hücrelere uygulandı. Sonuçlar uygulanan zaman ve konsantrasyona bağlı olarak EPI in L-hücrelerinin mitotik indeks değerlerini düşürdüğünü gösterdi. Bu düşüş kontrol grubu ile karşılaştırıldığında, her bir deney grubunda istatistik olarak anlamlı bulundu (p<0.05 - p<0.001).

Anahtar Kelime: fibroblast in vitro L-hücreleri hücre hatları mitoz mitotik indeks epirubisin

Konular: Biyokimya ve Moleküler Biyoloji

Epirubisinin kültürdeki L-hücrelerinde mitotik indekse etkisi

Öz:

Cancer chemotherapy is an additional application to surgical operations and radiotherapy in the treatment of widespread tumors. An anthracycline-derived antibiotic, epirubicin (EPI) is one of the clinically used antineoplastic drugs. In this study the cytotoxic effects of EPI in transformed mouse fibroblasts (L-cell) were examined. EPI concentrations of 0.001 µg/ml, 0.01 µg/ml and 0.1 µg/ml were applied to the cells for 2, 4, 8, 16 and 32 hours. The results showed that EPI diminished mitotic index of L-cells depends upon time and applied concentrations. This decrease was found statistically significant in each treatment group when compared to control (p<0.05 - p<0.001).

Anahtar Kelime: epirubicin fibroblasts in vitro L-cells cell lines mitosis mitotic index

Konular: Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ARICAN Ö, Topçul M (2003). Effect of epirubicin on mitotic index in cultured L-cells. , 43 - 48.
Chicago	ARICAN Özcan Gül,Topçul Mehmet Rıfkı Effect of epirubicin on mitotic index in cultured L-cells. (2003): 43 - 48.
MLA	ARICAN Özcan Gül,Topçul Mehmet Rıfkı Effect of epirubicin on mitotic index in cultured L-cells. , 2003, ss.43 - 48.
AMA	ARICAN Ö,Topçul M Effect of epirubicin on mitotic index in cultured L-cells. . 2003; 43 - 48.
Vancouver	ARICAN Ö,Topçul M Effect of epirubicin on mitotic index in cultured L-cells. . 2003; 43 - 48.
IEEE	ARICAN Ö,Topçul M "Effect of epirubicin on mitotic index in cultured L-cells." , ss.43 - 48, 2003.
ISNAD	ARICAN, Özcan Gül - Topçul, Mehmet Rıfkı. "Effect of epirubicin on mitotic index in cultured L-cells". (2003), 43-48.

APA	ARICAN Ö, Topçul M (2003). Effect of epirubicin on mitotic index in cultured L-cells. Journal of Cell and Molecular Biology, 2(1), 43 - 48.
Chicago	ARICAN Özcan Gül,Topçul Mehmet Rıfkı Effect of epirubicin on mitotic index in cultured L-cells. Journal of Cell and Molecular Biology 2, no.1 (2003): 43 - 48.
MLA	ARICAN Özcan Gül,Topçul Mehmet Rıfkı Effect of epirubicin on mitotic index in cultured L-cells. Journal of Cell and Molecular Biology, vol.2, no.1, 2003, ss.43 - 48.
AMA	ARICAN Ö,Topçul M Effect of epirubicin on mitotic index in cultured L-cells. Journal of Cell and Molecular Biology. 2003; 2(1): 43 - 48.
Vancouver	ARICAN Ö,Topçul M Effect of epirubicin on mitotic index in cultured L-cells. Journal of Cell and Molecular Biology. 2003; 2(1): 43 - 48.
IEEE	ARICAN Ö,Topçul M "Effect of epirubicin on mitotic index in cultured L-cells." Journal of Cell and Molecular Biology, 2, ss.43 - 48, 2003.
ISNAD	ARICAN, Özcan Gül - Topçul, Mehmet Rıfkı. "Effect of epirubicin on mitotic index in cultured L-cells". Journal of Cell and Molecular Biology 2/1 (2003), 43-48.