In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea
Yıl: 2021 Cilt: 18 Sayı: 2 Sayfa Aralığı: 92 - 99 Metin Dili: İngilizce DOI: 10.4274/tjps.galenos.2020.12258 İndeks Tarihi: 13-01-2022
In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea
Öz: Objectives: In Turkey, the genus Moltkia Lehm. is represented by two species, namely Moltkia aurea Boiss. and M. coerulea (Willd.) Lehm., which are used as both food and for medicinal purposes. This study aimed to evaluate the antidiabetic and antioxidant potential and phytochemical profiles of leaf, flower, and root extracts of Moltkia species. Materials and Methods: α-Glucosidase and α-amylase inhibitory activities, antioxidant effects, and total phenol and flavonoid contents of Moltkia extracts were evaluated. High-performance liquid chromatography was performed for identifying and quantifying phenolic compounds, which are responsible for various activities of these extracts. Results: Among the investigated phenolic compounds, caffeic and rosmarinic acids and rutin were determined and quantified in methanol extracts. Rutin was the major compound in the flower extract of M. aurea. Rutin and rosmarinic acid were the major compounds in the leaf extract of M. aurea. The flowers, leaves and roots of M. coerulea were also rich in rosmarinic acid. The antioxidant and antidiabetic potential of these extracts may be attributable to their rutin and rosmarinic acid content. Conclusion: Moltkia species can be used as natural sources of antioxidants. Notably, M. aurea extracts can be used for the development of herbal products with antidiabetic potential.
Anahtar Kelime: Moltkia aurea ve Moltkia coerulea’nın İn Vitro Enzim İnhibitör Özellikleri, Antioksidan Aktiviteleri ve Fitokimyasal Profilleri
Öz: Amaç: Moltkia Lehm. genusu Türkiye’de iki tür ile temsil edilmektedir: Moltkia aurea Boiss. ve M. coerulea (Willd.) Lehm. Her iki bitki de hem besin olarak hem de tıbbi amaçla kullanılmaktadır. Bu çalışma, Moltkia türlerinden elde edilen yaprak, çiçek ve kök ekstrelerinin antidiyabetik ve antioksidan potansiyellerini ve fitokimyasal profillerini incelemeyi amaçlamaktadır. Gereç ve Yöntemler: Moltkia ekstrelerinin α-glukozidaz ve α-amilaz enzim inhibitör aktiviteleri, antioksidan etkileri, total fenol ve flavonoit içerikleri değerlendirilmiştir. Bu ekstrelerin farklı aktivitelerinden sorumlu olabileceği düşünülen fenolik bileşiklerin belirlenmesi ve miktar tayini için yüksek performanslı sıvı kromatografisi yöntemi kullanılmıştır. Bulgular: İncelenen fenolik bileşikler arasında, kafeik asit, rozmarinik asit ve rutin metanol ekstrelerinde tespit edilmiş ve miktarları tayin edilmiştir. Rutin, M. aurea çiçek ekstresinin ana bileşeni olarak belirlenmiştir. Rutin ve rosmarinik asit M. aurea yaprak ekstresinin ana bileşenleri olarak belirlenmiştir. M. coerulea çiçek, yaprak ve köklerinin ise rozmarinik asit açısından da zengin olduğu tespit edilmiştir. Ekstrelerin antioksidan ve antidiyabetik etkileri rutin ve rozmarinik asit içeriklerine bağlı olabilir. Sonuç: Moltkia türleri potansiyel doğal antioksidan kaynağı olarak kullanılabilir. Özellikle M. aurea ekstreleri antidiyabetik potansiyele sahip bitkisel ürün geliştirilmesinde kullanılabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Dogu S, Dinc M, Pınar NM. Anatomical and micromorphological differentiation in the genus Moltkia Lehm in Turkey. Pakistan J Bot. 2012;44:1083-1090.
- Sharma RA, Singh B, Singh D, Chandrawat P. Ethnomedicinal, pharmacological properties and chemistry of some medicinal plants of Boraginaceae in India. J Medic Plants Res. 2009;3:1153-1175.
- Balpınar N, Ökmen G. The biological activities of Moltkia aurea Boiss., an endemic species to Turkey. Afr J Tradit Complement Altern Med. 2017;14:60-64.
- Georgiou O, Dimitrellos G, Georgiadis T. Moltkia petraea (Boraginaceae) in Greece. Phyton. 2000;40:57-69.
- Harput US, Nagatsu A, Saracoglu, I. Antioxidant and cytotoxic effects of Moltkia aurea Boiss. Rec Nat Prod. 2012;6:62-66.
- Ozdemir E, Alpınar K. The wild edible plants of western Nigde Aladaglar Mountains (Central Turkey). Istanbul J Pharm. 2010-2011;41:66-74.
- Ozdemir E, Alpınar K. An ethnobotanical survey of medicinal plants in western part of central Taurus Mountains: Aladaglar (Nigde-Turkey). J Ethnopharmacol. 2015;166:53-65.
- Van Quan N, Xuan TD, Tran HD, Thuy NTD, Trang LT, Huong CT, Andriana Y, Tuyen PT. Antioxidant, α-amylase and α-glucosidase inhibitory activities and potential constituents of Canarium tramdenum Bark, Molecules. 2019;24:605.
- Zengin G, Ceylan R, Katanic J, Mollica A, Aktumsek A, Boroja T, Matic S, Mihailovic V, Stanic S, Aumeerudy-Elalfi Z, Yılmaz MA, Mahomoodally MF. Combining in vitro, in vivo and in silico approaches to evaluate nutraceutical potentials and chemical fingerprints of Moltkia aurea and Moltkia coerulea. Food Chem Toxicol. 2017;107:540-553.
- Ali H, Houghton PJ, Soumyanath A. α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes, with particular reference to Phyllanthus amarus. J Ethnopharmacol. 2006;107:449-455.
- Lam SH, Chen JM, Kang CJ, Chen CH, Lee SS. α-Glucosidase inhibitors from the seeds of Syagrus romanzoffiana. Phytochemistry. 2008;69:1173- 1178.
- Jung HA, Jin SE, Choi RJ, Manh HT, Kim YS, Min BS, Son YK, Ahn BR, Kim BW, Sohn HS, Choi JS. Anti tumorigenic activity of sophoraflavescenol against lewis lung carcinoma in vitro and in vivo. Arch Pharm Res. 2011;34:2087-2099.
- Liu F, Ooi VFC, Chang ST. Free radical scavenging activity of mushroom polysaccharide extracts. Life Sci. 1997;60:763-771.
- Adaramoye OA, Farombi EO, Adeyemi EO, Emerole GO. Comparative study on the antioxidant properties of flavonoids of Garcinia kola seeds. Pak J Med Sci. 2005;21:331-339.
- Oyaizu M. Studies on products of browning reactions-antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr. 1986;44:307-315.
- Dinish TCP, Madeira VMC, Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys. 1994;315:161-169.
- Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex, specific application to the determination of vitamin E. Anal Biochem. 1999;269:337-341.
- Zongo C, Savadogo A, Ouattara L, Bassole IHN, Ouattara CAT, Ouattara AS, Barro N, Koudou J, Traore AS. Polyphenols content, antioxidant and antimicrobial activities of Ampelocissus grantii (Baker) Planch. (Vitaceae): A medicinal plant from Burkina Faso. Int J Pharmacol. 2010;6:880-887.
- Kosalec I, Bakmaz M, Pepeljnjak S, Vladimir-Knezevic S. Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharmaceut. 2004;54:65-72.
- Gökbulut A. Validated RP-HPLC method for quantification of phenolic compounds in methanol extracts of aerial parts and roots of Thymus sipyleus and evaluation of antioxidant potential. Trop J Pharm Res. 2015;14:1871-1877.
- Orhan Deliorman D, Hartevioğlu A, Orhan N, Berkkan A, Gökbulut A, Günhan Ö, Pekcan M. Subacute effects of standardized Fumaria vaillantii Lois. ethanol extract on trace element levels, biochemical and histopathological parameters in experimental liver toxicity. J Food Biochem. 2016;40:180-189.
- Meot-Duros L, Le Floch G, Magne C. Radical scavenging, antioxidant and antimicrobial activities of halophytic species. J Ethnopharmacol. 2008;116:258-262.
- Gullón B, Lú-Chau TA, Moreira MT, Lema JM, Eibes G. Rutin: A review on extraction, identification and purification methods, biological activities and approaches to enhance its bioavailability. Trends Food Sci Tech. 2017;67:220-235.
- Al-Dhabi NA, Arasu MV, Park CH, Park SU. Recent studies on rosmarinic acid and its biological and pharmacological activities. EXCLI J. 2014;13:1192-1195.
- Erdemoglu N, Kusmenoglu S, Vural M. γ-Linolenic acid content and fatty acid composition of Boraginaceae seed oils. Eur J Lipid Sci Tech. 2004;106:160-164.
- Farahpour MR, Dilmaghanian A, Faridy M, Karashi E. Topical Moltkia coerulea hydroethanolic extract accelerates the repair of excision wound in a rat model. Chin J Traumatol. 2016;19:97-103.
- Meshkatalsadat MH, Parekh HH. Chemical investigations of the flowers of Moltkia coerulea. Planta Med. 1990;56:556-557.
| APA | ORHAN N, Gökbulut A, Deliorman Orhan D (2021). In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea. , 92 - 99. 10.4274/tjps.galenos.2020.12258 |
| Chicago | ORHAN Nilufer,Gökbulut Alper,Deliorman Orhan Didem In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea. (2021): 92 - 99. 10.4274/tjps.galenos.2020.12258 |
| MLA | ORHAN Nilufer,Gökbulut Alper,Deliorman Orhan Didem In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea. , 2021, ss.92 - 99. 10.4274/tjps.galenos.2020.12258 |
| AMA | ORHAN N,Gökbulut A,Deliorman Orhan D In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea. . 2021; 92 - 99. 10.4274/tjps.galenos.2020.12258 |
| Vancouver | ORHAN N,Gökbulut A,Deliorman Orhan D In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea. . 2021; 92 - 99. 10.4274/tjps.galenos.2020.12258 |
| IEEE | ORHAN N,Gökbulut A,Deliorman Orhan D "In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea." , ss.92 - 99, 2021. 10.4274/tjps.galenos.2020.12258 |
| ISNAD | ORHAN, Nilufer vd. "In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea". (2021), 92-99. https://doi.org/10.4274/tjps.galenos.2020.12258 |
| APA | ORHAN N, Gökbulut A, Deliorman Orhan D (2021). In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea. Turkish Journal of Pharmaceutical Sciences, 18(2), 92 - 99. 10.4274/tjps.galenos.2020.12258 |
| Chicago | ORHAN Nilufer,Gökbulut Alper,Deliorman Orhan Didem In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea. Turkish Journal of Pharmaceutical Sciences 18, no.2 (2021): 92 - 99. 10.4274/tjps.galenos.2020.12258 |
| MLA | ORHAN Nilufer,Gökbulut Alper,Deliorman Orhan Didem In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea. Turkish Journal of Pharmaceutical Sciences, vol.18, no.2, 2021, ss.92 - 99. 10.4274/tjps.galenos.2020.12258 |
| AMA | ORHAN N,Gökbulut A,Deliorman Orhan D In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea. Turkish Journal of Pharmaceutical Sciences. 2021; 18(2): 92 - 99. 10.4274/tjps.galenos.2020.12258 |
| Vancouver | ORHAN N,Gökbulut A,Deliorman Orhan D In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea. Turkish Journal of Pharmaceutical Sciences. 2021; 18(2): 92 - 99. 10.4274/tjps.galenos.2020.12258 |
| IEEE | ORHAN N,Gökbulut A,Deliorman Orhan D "In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea." Turkish Journal of Pharmaceutical Sciences, 18, ss.92 - 99, 2021. 10.4274/tjps.galenos.2020.12258 |
| ISNAD | ORHAN, Nilufer vd. "In Vitro Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profiles of Moltkia aurea and Moltkia coerulea". Turkish Journal of Pharmaceutical Sciences 18/2 (2021), 92-99. https://doi.org/10.4274/tjps.galenos.2020.12258 |
