Effects of Saint John’s Wort extract on intestinal injury and apoptosis

SEHİRLİ, Ahmet Ozer; SEHIRLI, AHMET OZER; Gül, Mehmet Onur; Sunamak, Oguzhan; akyüz, cebrail

Effects of Saint John’s Wort extract on intestinal injury and apoptosis

Mehmet Onur GÜL, (Çukurova Üniversitesi, Balcalı Eğitim ve Araştırma Hastanesi, Cerrahi Onkoloji Anabilim Dalı, Adana, Türkiye)

Cebrail AKYÜZ, (Gastroenterolojik Cerrahi Anabilim Dalı; Haydarpaşa Numune Eğitim ve Araştırma Hastanesi, İstanbul, Türkiye)

Oğuzhan SUNAMAK, (Haydarpaşa Numune Eğitim ve Araştırma Hastanesi Genel Cerrahi Kliniği, İstanbul, Türkiye)

Ahmet ŞEHİRLİ, (Sağlık Meslek Yüksekokulu, Marmara Üniversitesi, İstanbul, Türkiye)

Ahmet Ozer Sehirli (Farmakoloji Anabilim Dalı, Diş Hekimliği Fakültesi, Yakın Doğu Üniversitesi, Lefkoşa, Kıbrıs)

Annals of Medical Research

4 2

Yıl: 2021 Cilt: 28 Sayı: 9 Sayfa Aralığı: 1725 - 1730 Metin Dili: İngilizce İndeks Tarihi: 17-01-2022

Effects of Saint John’s Wort extract on intestinal injury and apoptosis

Öz:

Aim: Reactive oxygen species play an important role in the pathophysiology of intestinal ischemia/reperfusion (I/R) injury by causing apoptosis. The present study aimed at exploring the possible protective effect of Saint John’s Wort (SJW) extract in I/R injury. Materials and Methods: Twenty four healthy male Wistar rats of 6 to 8 weeks old weighting averagely 200 to 250 g were studied. They were fed on standard rat food and drinking water at room temperature with 12/12 hours periods of day and night. SJW (300 mg/kg, peroral) or saline was given by oral route for 3 days prior to ischemia, 30 minutes before the ischemia, and just before reperfusion. To the rats in the control group, sham operation and application of saline were done. Levels of TNF-α, IL-1β and LDH were measured in the serum samples. In the small bowel tissue, levels of malonaldehyde (MDA) and glutathione (GSH) and activity of myeloperoxidase (MPO) and Na-K ATPase and level of caspase-3/β-actine and Bcl-2/β-actine were measured. Results: I/R increased serum levels of LDH, TNF-α and IL-1β, small bowel level of MDA and MPO whereas it decreased level of GSH and activity of Na-K ATPase in the small bowel tissue. The level of caspase-3/β-actine increased while the level of Bcl-2/β-actine decreased in the I/R group compared to the controls. With the application of SJW, these values approached the control levels. Conclusion: These results indicate that SJW recovers small bowel function by reducing oxidation injury during I/R.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Gül M, akyüz c, Sunamak O, SEHIRLI A, SEHİRLİ A (2021). Effects of Saint John’s Wort extract on intestinal injury and apoptosis. , 1725 - 1730.
Chicago	Gül Mehmet Onur,akyüz cebrail,Sunamak Oguzhan,SEHIRLI AHMET OZER,SEHİRLİ Ahmet Ozer Effects of Saint John’s Wort extract on intestinal injury and apoptosis. (2021): 1725 - 1730.
MLA	Gül Mehmet Onur,akyüz cebrail,Sunamak Oguzhan,SEHIRLI AHMET OZER,SEHİRLİ Ahmet Ozer Effects of Saint John’s Wort extract on intestinal injury and apoptosis. , 2021, ss.1725 - 1730.
AMA	Gül M,akyüz c,Sunamak O,SEHIRLI A,SEHİRLİ A Effects of Saint John’s Wort extract on intestinal injury and apoptosis. . 2021; 1725 - 1730.
Vancouver	Gül M,akyüz c,Sunamak O,SEHIRLI A,SEHİRLİ A Effects of Saint John’s Wort extract on intestinal injury and apoptosis. . 2021; 1725 - 1730.
IEEE	Gül M,akyüz c,Sunamak O,SEHIRLI A,SEHİRLİ A "Effects of Saint John’s Wort extract on intestinal injury and apoptosis." , ss.1725 - 1730, 2021.
ISNAD	Gül, Mehmet Onur vd. "Effects of Saint John’s Wort extract on intestinal injury and apoptosis". (2021), 1725-1730.

APA	Gül M, akyüz c, Sunamak O, SEHIRLI A, SEHİRLİ A (2021). Effects of Saint John’s Wort extract on intestinal injury and apoptosis. Annals of Medical Research, 28(9), 1725 - 1730.
Chicago	Gül Mehmet Onur,akyüz cebrail,Sunamak Oguzhan,SEHIRLI AHMET OZER,SEHİRLİ Ahmet Ozer Effects of Saint John’s Wort extract on intestinal injury and apoptosis. Annals of Medical Research 28, no.9 (2021): 1725 - 1730.
MLA	Gül Mehmet Onur,akyüz cebrail,Sunamak Oguzhan,SEHIRLI AHMET OZER,SEHİRLİ Ahmet Ozer Effects of Saint John’s Wort extract on intestinal injury and apoptosis. Annals of Medical Research, vol.28, no.9, 2021, ss.1725 - 1730.
AMA	Gül M,akyüz c,Sunamak O,SEHIRLI A,SEHİRLİ A Effects of Saint John’s Wort extract on intestinal injury and apoptosis. Annals of Medical Research. 2021; 28(9): 1725 - 1730.
Vancouver	Gül M,akyüz c,Sunamak O,SEHIRLI A,SEHİRLİ A Effects of Saint John’s Wort extract on intestinal injury and apoptosis. Annals of Medical Research. 2021; 28(9): 1725 - 1730.
IEEE	Gül M,akyüz c,Sunamak O,SEHIRLI A,SEHİRLİ A "Effects of Saint John’s Wort extract on intestinal injury and apoptosis." Annals of Medical Research, 28, ss.1725 - 1730, 2021.
ISNAD	Gül, Mehmet Onur vd. "Effects of Saint John’s Wort extract on intestinal injury and apoptosis". Annals of Medical Research 28/9 (2021), 1725-1730.