Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars

YORULMAZ, ALPER; AYDIN, MEHMET; DEMİRAL, Mehmet Ali

Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars

Mehmet Ali DEMİRAL, (Adnan Menderes Üniversitesi, Ziraat Fakültesi, Aydın, Türkiye)

Mehmet AYDIN, (Adnan Menderes Üniversitesi, Ziraat Fakültesi, Aydın, Türkiye)

Alper YORULMAZ (Adnan Menderes Üniversitesi, Ziraat Fakültesi, Aydın, Türkiye)

Turkish Journal of Biology

1 0

Yıl: 2005 Cilt: 29 Sayı: 2 Sayfa Aralığı: 117 - 123 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars

Öz:

Tuzluluğun iki farklı arpa {Hordeum vulgare L.) çeşidinde (Kaya ve Scarlet) gelişim, kimyasal içerik, süperokside dismutase (SOD) ve peroksidase (P0X) aktiviteleri üzerine olan etkileri araştırıldı. Tuz stresi, fidelerin 10 günlük olduğu dönemde uygun miktarlarda NaCl karıştırılmış su verilmesiyle başlatıldı. Tuz uygulaması çeşitlerin gelişimlerini önemli ölçüde geriletti. Scarlet çeşidi Kaya çeşidine göre $Na^+$ ve $Cl^-$ elementlerinin köke girişini ve yapraklara taşınmasını daha iyi sınırlandırdı. Tuz stresi altında genel olarak Scarlet çeşidi Kaya'ya göre daha yüksek miktarlarda klorofil üretti. Çeşitlerin (P0X) aktiviteleri, 20 dS $m^{-1}$ tuz düzeyine kadar azaldı ve daha sonra arttı. POX aktivitesinin aksine, tuzluluk yaprakların SOD içeriğini arttırdı. Kontrole göre 10 dS $m^{-1}$ tuz düzeyinde SOD aktivitesi Kaya çeşidinde %5,31 ve Scarlet çeşidinde %16,34 artış gösterdi.

Anahtar Kelime: peroksidaz süperoksit dismutaz kimyasal bileşim Hordeum vulgare arpa tuzluluk protein tuz toleransı enzim aktivitesi klorofil büyüme

Konular: Biyoloji

Tuzluluğun iki maltlık arpa (Hordeum vulgare L.) çeşidinde gelişim kimyasal bileşim ve antioksidatif enzim aktivitesi üzerine etkisi

Öz:

The effects of salinity on the growth, chemical composition and superoxide dismutase (SOD) and peroxidase (POX) activities of 2 malting barley (Hordeum vulgare L.) cultivars (Kaya and Scarlet) were studied. In 10-day-old seedlings, salinity stress was initiated by applying an appropriate amount of NaCl to water, and it lasted for 30 days. Salinity decreased growth of the cultivars significantly. Scarlet restricted entry of $Na^+$ and $Cl^-$ into root and translocation to leaves more efficiently than did Kaya. As a whole Scarlet produced a higher amount total chlorophyll under salinity than did Kaya. POX activity of the cultivars decreased with salinity up to 20 dS $m^{-1}$ , and then increased. In contrast to POX, salinity increased the SOD activity of leaves. Compared to the control application, 5.31% and 16.34% increases were determined in SOD activity, in 10 dS $m^{-1}$ salinity, for Kaya and Scarlet,

Anahtar Kelime: salt tolerance enzyme activity chlorophyll growth peroxidase superoxide dismutase chemical composition Hordeum vulgare barley salinity protein

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Rhoades JD, Loveday J. Salinity in Irrigated Agriculture. Steward, BA, Nielsen DR eds. American Society of Civil Engineers, Irrigation of Agricultural Crops Am Soc Agronomists, Monograph 30; 1990: pp. 1089-1142.
2. Xiong L, Zhu JK. Salt tolerance. The Arabidopsis Book. American Society of Plant Biologists 1: 1 -22, 2002.
3. Bağcı SA, Ekiz H, Yılmaz A. Determination of the salt tolerance of some barley genotypes and the characteristics affecting tolerance. Turk J Agric For 27: 253-260, 2003.
4. Ali A, Tucker TC, Thompson TL et al. Effects of salinity and mixed ammonium and nitrate nutrition on the growth and nitrogen utilization of barley. J. Agronomy & Crop Science 186: 223-228,2001.
5. Hoagland DR, Arnon DI. The Water Culture Method for Growing Plants Without Soil. Circular 347, College of Agriculture, University of California, Berkeley; 1950.
6. Littel TM, Hills FJ. Agricultural Experimentation, Design and Analysis. John Wiley and Sons Inc., N.Y, 1978.
7. Kaçar B. Bitki ve Topragin Kimyasal Analizleri, II. Bitki Analizleri. Ankara Üniversitesi Ziraat Fakültesi Yayinlari, No. 453, Ankara, Turkey; 1972.
8. Lichtenthaler HK, Wellburn AR. Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Transac 11: 591-592, 1983.
9. Beauchamp C, Fridovich 1. Superoxide dismutase; Improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276-287,1971.
10. Herzog V, Fahimi H. A new sensitive colorimetric assay for peroxide using 3-3'- diamineobenz-idine as hydrogen donor. Analytical Biochemistry 55: 554-562, 1973.
11. Lowry OH, Rosebrough NJ, Farr AL et al. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-275, 1951.
12. Mer RK, Prajith PK, Pandya DH et al. Effects of salts on germination of seeds and growth of young plants of Hordeum vulgare, Triticum aestium, Cicer aestivum and Brassica juncea. J Agronomy & Crop Science 185: 209-217, 2000.
13. Hussain G, Al-Jaloudi AA, Al-Shommary SA et al. Effect of saline irrigation on germination and growth parameters of barley {Hordeum vulgare L.) in a pot experiment. Agriculture Water Management 34: 125-135, 1997.
14. İsla R, Aragues R, Royo A. Validity of various physiological traits as screening criteria for salt tolerance in barley. Field Crops Research 58: 97-107, 1998.
15. Lazof DB, Bernstein N. The NaCl induced inhibition of shoot growth, the case for disturbed nutrition with special consideration of calcium nutrition. Adv Bot Res 29: 113-189, 1999.
16. Nublat A, Desplans J, Casse F. et al. Sasl, an Arabidopsis mutant over accumulating sodium in the shoot, shows deficiency in the control of the root redial transport of sodium. Plant Cell 13:125- 137,2001.
17. Mengel K, Kirkby EA. Principles of Plant Nutrition. 3rd Edition. International Potash Institute. P.O. Box, CH-3048, Worblaufen- Bern, Switzerland; 1982.
18. Jacoby B. Mechanisms involved in salt tolerance of plants. In: Pessarakli M. ed. Handbook of Plant and Crop Stress. 2nd Edition. Marcel Dekker Inc., New York; 1999: pp. 97-123.
19. Ashraf M. Salt tolerance of cotton: Some new advances. Critical Review in Plant Sciences, 21:1-30, 2002.
20. Zidan I, Jacoby B, Ravina I et al. Sodium does not compete with calcium in saturating plasma membrane sites regulating Z2Na influx in salinized maize roots. Plant Physiol 96: 331, 1991.
21. Lahaye PA, Epstein E. Calcium and salt toleration by bean plants. Physiol Plant 25: 213, 1971.
22. Ben-Hayin G, Kafkafi U, Ganmore-Neumann R. Role of internal potassium in maintaining growth of cultured citrus cells on increasing NaCl and CaCl2 concentration. Plant Physiol 85: 434,1987.
23. Kawasaki S, Borchert C, Deyholos M et al. Gene expression profiles during the initial phase of salt stress in rice. Plant Cell. 13:889-905,2001.
24. White TCR. The abundance of invertebrate herbivores in relation to the availability of nitrogen in stressed food plants. Oecologia 63: 90-105, 1984.
25. Showier AT, Moran PJ. Effects of drought stressed cotton, Gossypium hirsutum L., on beet armyworm, Spodoptera exigua. (Hübner), oviposition, and larval feeding references and growth. Journal of Chemical Ecology Vol. 29, No.9, 2003.
26. Acar O, Türkan I, Özdemir F. Superoxide dismutase and peroxidase activities in drought sensitive and resistant barley (Hordeum vulgare L.) varieties. Acta Physiologiae Piantarum 23: 351-356,2001.
27. Alscher RG, Ertürk N, Health LS. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany, Vol. 53, No. 372. Antioxidants and reactive oxygen species in plants. Special Issue, pp. 1331-1341, 2002.
28. Fridovich I. Superoxide dismutases. Advances in Enzymology and Related Areas of Molecular Biology, 58: 61-97, 1986.

APA	DEMİRAL M, AYDIN M, YORULMAZ A (2005). Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. , 117 - 123.
Chicago	DEMİRAL Mehmet Ali,AYDIN MEHMET,YORULMAZ ALPER Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. (2005): 117 - 123.
MLA	DEMİRAL Mehmet Ali,AYDIN MEHMET,YORULMAZ ALPER Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. , 2005, ss.117 - 123.
AMA	DEMİRAL M,AYDIN M,YORULMAZ A Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. . 2005; 117 - 123.
Vancouver	DEMİRAL M,AYDIN M,YORULMAZ A Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. . 2005; 117 - 123.
IEEE	DEMİRAL M,AYDIN M,YORULMAZ A "Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars." , ss.117 - 123, 2005.
ISNAD	DEMİRAL, Mehmet Ali vd. "Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars". (2005), 117-123.

APA	DEMİRAL M, AYDIN M, YORULMAZ A (2005). Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. Turkish Journal of Biology, 29(2), 117 - 123.
Chicago	DEMİRAL Mehmet Ali,AYDIN MEHMET,YORULMAZ ALPER Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. Turkish Journal of Biology 29, no.2 (2005): 117 - 123.
MLA	DEMİRAL Mehmet Ali,AYDIN MEHMET,YORULMAZ ALPER Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. Turkish Journal of Biology, vol.29, no.2, 2005, ss.117 - 123.
AMA	DEMİRAL M,AYDIN M,YORULMAZ A Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. Turkish Journal of Biology. 2005; 29(2): 117 - 123.
Vancouver	DEMİRAL M,AYDIN M,YORULMAZ A Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. Turkish Journal of Biology. 2005; 29(2): 117 - 123.
IEEE	DEMİRAL M,AYDIN M,YORULMAZ A "Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars." Turkish Journal of Biology, 29, ss.117 - 123, 2005.
ISNAD	DEMİRAL, Mehmet Ali vd. "Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars". Turkish Journal of Biology 29/2 (2005), 117-123.