Moleküler Kimerizm Metotları: Geçmiş ve Günümüz

SAVRAN OGUZ, FATMA; Abatay Sel, Figen

doi:10.53394/akd.1037771

Moleküler Kimerizm Metotları: Geçmiş ve Günümüz

Figen ABATAY SEL, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İstanbul, Türkiye)

Fatma SAVRAN OĞUZ (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İstanbul, Türkiye)

Akdeniz Tıp Dergisi

2 1

Yıl: 2022 Cilt: 8 Sayı: 1 Sayfa Aralığı: 82 - 90 Metin Dili: Türkçe DOI: 10.53394/akd.1037771 İndeks Tarihi: 29-07-2022

Moleküler Kimerizm Metotları: Geçmiş ve Günümüz

Öz:

Kimerizm analizi, hematolojide allojenik hematopoetik kök hücre transplantasyonu (AHKHT) sonrası hastadaki donör hücre miktarının tespiti için kullanılan bir tanı yöntemidir. Transplantasyon sonrası yapılan kimerizm analizi, donör hücre engraftmanı ve hastalığın relapsı açısından oldukça önemli bilgiler vermektedir. Kimerizm analizi, çeşitli belirteçler ve teknikler kullanılarak yapılmaktadır. Son zamanlarda kantitatif-gerçek zamanlı polimeraz zincir reaksiyonu (qRT-PCR), dijital droplet-PCR (dd-PCR) ve yeni nesil dizileme (NGS) gibi yeni kimerizm yöntemleri kullanılmaktadır. Bu derlemede multipleks kısa ardışık tekrarlı dizi tabanlı PCR (STR-PCR) yöntemi ile yeni moleküler qRT-PCR, dd-PCR ve NGS yöntemlerinin avantajlı ve dezavantajlı yönleri karşılaştırılıp tartışılmıştır.

Anahtar Kelime: dd-PCR qRT-PCR Kimerizm analizi NGS STR-PCR

Molecular Chimerism Methods: Past and Present

Öz:

Chimerism analysis is a diagnostic method used to determine the amount of donor cells in the patient for hematologic cases after allogeneic hematoietic stem cell transplantation (AHSCT). Post-transplant chimerism analysis provides very important information in terms of donor cell engraftment and relapse. Chimerism analysis is performed using various markers and techniques. Recently, new chimerism methods have been used such as quantitative-real time polymerase chain reaction (qRT-PCR), digital droplet-PCR (dd-PCR) and next generation sequencing (NGS). In this review, multiplex short tandem repeat based-PCR (STR-PCR) and new molecular methods qRT-PCR, dd-PCR and NGS were compared and discussed in the terms of advantageous and disadvantageous aspects.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Derleme Erişim Türü: Erişime Açık

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APA	Abatay Sel F, SAVRAN OGUZ F (2022). Moleküler Kimerizm Metotları: Geçmiş ve Günümüz. , 82 - 90. 10.53394/akd.1037771
Chicago	Abatay Sel Figen,SAVRAN OGUZ FATMA Moleküler Kimerizm Metotları: Geçmiş ve Günümüz. (2022): 82 - 90. 10.53394/akd.1037771
MLA	Abatay Sel Figen,SAVRAN OGUZ FATMA Moleküler Kimerizm Metotları: Geçmiş ve Günümüz. , 2022, ss.82 - 90. 10.53394/akd.1037771
AMA	Abatay Sel F,SAVRAN OGUZ F Moleküler Kimerizm Metotları: Geçmiş ve Günümüz. . 2022; 82 - 90. 10.53394/akd.1037771
Vancouver	Abatay Sel F,SAVRAN OGUZ F Moleküler Kimerizm Metotları: Geçmiş ve Günümüz. . 2022; 82 - 90. 10.53394/akd.1037771
IEEE	Abatay Sel F,SAVRAN OGUZ F "Moleküler Kimerizm Metotları: Geçmiş ve Günümüz." , ss.82 - 90, 2022. 10.53394/akd.1037771
ISNAD	Abatay Sel, Figen - SAVRAN OGUZ, FATMA. "Moleküler Kimerizm Metotları: Geçmiş ve Günümüz". (2022), 82-90. https://doi.org/10.53394/akd.1037771

APA	Abatay Sel F, SAVRAN OGUZ F (2022). Moleküler Kimerizm Metotları: Geçmiş ve Günümüz. Akdeniz Tıp Dergisi, 8(1), 82 - 90. 10.53394/akd.1037771
Chicago	Abatay Sel Figen,SAVRAN OGUZ FATMA Moleküler Kimerizm Metotları: Geçmiş ve Günümüz. Akdeniz Tıp Dergisi 8, no.1 (2022): 82 - 90. 10.53394/akd.1037771
MLA	Abatay Sel Figen,SAVRAN OGUZ FATMA Moleküler Kimerizm Metotları: Geçmiş ve Günümüz. Akdeniz Tıp Dergisi, vol.8, no.1, 2022, ss.82 - 90. 10.53394/akd.1037771
AMA	Abatay Sel F,SAVRAN OGUZ F Moleküler Kimerizm Metotları: Geçmiş ve Günümüz. Akdeniz Tıp Dergisi. 2022; 8(1): 82 - 90. 10.53394/akd.1037771
Vancouver	Abatay Sel F,SAVRAN OGUZ F Moleküler Kimerizm Metotları: Geçmiş ve Günümüz. Akdeniz Tıp Dergisi. 2022; 8(1): 82 - 90. 10.53394/akd.1037771
IEEE	Abatay Sel F,SAVRAN OGUZ F "Moleküler Kimerizm Metotları: Geçmiş ve Günümüz." Akdeniz Tıp Dergisi, 8, ss.82 - 90, 2022. 10.53394/akd.1037771
ISNAD	Abatay Sel, Figen - SAVRAN OGUZ, FATMA. "Moleküler Kimerizm Metotları: Geçmiş ve Günümüz". Akdeniz Tıp Dergisi 8/1 (2022), 82-90. https://doi.org/10.53394/akd.1037771