Chemical profile by LC-Q-TOF-MS of Nigella sativa seed
extracts and in vitro antimicrobial activity on bacteria
which are determined resistance gene and isolated from
nosocomial infection

SERVI, HÜSEYIN; AYSAL, Ayhan Ibrahim; KISA, ÖZGÜL; Satana, Dilek

doi:10.29228/jrp.127

Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection

Hüseyin SERVİ, (Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, İstanbul Yeni Yüzyıl Üniversitesi, İstanbul, Türkiye)

Özgül KISA, (Ufuk Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Ayhan Ibrahim AYSAL, (Farmasötik Kimya Anabilim Dalı, Eczacılık Fakültesi, Gazi Üniversitesi, Ankara, Türkiye)

Dilek ŞATANA (İstanbul Üniversitesi Tıp Fakültesi Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye)

Journal of research in pharmacy (online)

12 0

Yıl: 2022 Cilt: 26 Sayı: 2 Sayfa Aralığı: 287 - 297 Metin Dili: İngilizce DOI: 10.29228/jrp.127 İndeks Tarihi: 07-06-2022

Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection

Öz:

Healthcare-associated infection (HCAI) is a global health challenge and methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum beta-lactamases (ESBL) producing gram-negative bacilli, which were isolated from HCAIs, are the most common antimicrobial-resistant pathogens. The present research aimed to determine the chemical composition and antimicrobial activity of ethyl acetate and methanol extracts of Nigella sativa seeds. Antimicrobial activity of plant extracts was observed against 20 clinical yeast (which isolated from swab samples of tongue dorsal surfaces in patients with stomatitis), 18 clinical bacteria isolates (which found from HCAIs), and also eight bacteria, and three yeast standard strains by using a broth microdilution assay. The methanol extract had the highest inhibitory activity against S. aureus standard strain at 0.4 mg/mL. The ethyl acetate extract showed the highest antibacterial activity against Bacillus cereus standard strain at 0.4 mg/mL. The methanol and ethyl acetate extracts did not show inhibitory activity against Gram-negative bacteria and yeast isolates. The LC-Q-TOF-MS profiles of the extracts were found to contain saponin, alkaloid, and fatty acid. Mass spectra revealed the presence of 14 fatty acids, four saponins, and three alkaloids for methanol extract and 13 fatty acids, three saponins, and two alkaloids for ethyl acetate extract.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SERVI H, KISA Ö, AYSAL A, Satana D (2022). Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. , 287 - 297. 10.29228/jrp.127
Chicago	SERVI HÜSEYIN,KISA ÖZGÜL,AYSAL Ayhan Ibrahim,Satana Dilek Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. (2022): 287 - 297. 10.29228/jrp.127
MLA	SERVI HÜSEYIN,KISA ÖZGÜL,AYSAL Ayhan Ibrahim,Satana Dilek Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. , 2022, ss.287 - 297. 10.29228/jrp.127
AMA	SERVI H,KISA Ö,AYSAL A,Satana D Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. . 2022; 287 - 297. 10.29228/jrp.127
Vancouver	SERVI H,KISA Ö,AYSAL A,Satana D Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. . 2022; 287 - 297. 10.29228/jrp.127
IEEE	SERVI H,KISA Ö,AYSAL A,Satana D "Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection." , ss.287 - 297, 2022. 10.29228/jrp.127
ISNAD	SERVI, HÜSEYIN vd. "Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection". (2022), 287-297. https://doi.org/10.29228/jrp.127

APA	SERVI H, KISA Ö, AYSAL A, Satana D (2022). Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. Journal of research in pharmacy (online), 26(2), 287 - 297. 10.29228/jrp.127
Chicago	SERVI HÜSEYIN,KISA ÖZGÜL,AYSAL Ayhan Ibrahim,Satana Dilek Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. Journal of research in pharmacy (online) 26, no.2 (2022): 287 - 297. 10.29228/jrp.127
MLA	SERVI HÜSEYIN,KISA ÖZGÜL,AYSAL Ayhan Ibrahim,Satana Dilek Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. Journal of research in pharmacy (online), vol.26, no.2, 2022, ss.287 - 297. 10.29228/jrp.127
AMA	SERVI H,KISA Ö,AYSAL A,Satana D Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. Journal of research in pharmacy (online). 2022; 26(2): 287 - 297. 10.29228/jrp.127
Vancouver	SERVI H,KISA Ö,AYSAL A,Satana D Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. Journal of research in pharmacy (online). 2022; 26(2): 287 - 297. 10.29228/jrp.127
IEEE	SERVI H,KISA Ö,AYSAL A,Satana D "Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection." Journal of research in pharmacy (online), 26, ss.287 - 297, 2022. 10.29228/jrp.127
ISNAD	SERVI, HÜSEYIN vd. "Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection". Journal of research in pharmacy (online) 26/2 (2022), 287-297. https://doi.org/10.29228/jrp.127