Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease
Yıl: 2022 Cilt: 50 Sayı: 1 Sayfa Aralığı: 22 - 33 Metin Dili: İngilizce DOI: 10.5543/tkda.2022.17203 İndeks Tarihi: 17-06-2022
Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease
Öz: Objective: Coronary artery disease (CAD) is an important public health problem worldwide. Therefore, it is important to identify the molecular mechanisms and the candidate gene polymorphisms involved in the development of CAD. In this study, we focused on 2 polymorphisms of the atherosclerosis-related genes, ESR1 and CYP19A1.
Methods: Unselected 339 individuals who underwent coronary angiography were divided into 2 groups: those with normal coronary arteries (≤30% stenosis) and those with critical disease (≥50% stenosis). Individuals were genotyped for CYP19A1 rs10046 C/T and ESR1 rs2175898 A/G polymorphisms using hybridization probes in real-time PCR. In addition, Gensini and SYNTAX scores were assessed.
Results: ESR1 polymorphism was significantly associated with CAD in men (p=0.036) via G allele carriage. Multiple logistic regression analyses showed that ESR1 rare allele carriage was associated with CAD presence (Odds ratio=2.12, 95% confidence interval 1.01–4.1, p=0.025), adjusted for age, HDL-C, LDL-C and smoking status in the male group. CYP19A1 rs10046 T allele carriers had a 2.84-fold increased risk for complex CAD in multiple logistic regression analysis (p=0.016). Furthermore, the univariate analysis of variance indicated that T allele carriage of rs10046 polymorphism was associated with increased SYNTAX and Gensini scores (p<0.05). Female patients who were ESR1 G allele carriers with CAD had higher adiponectin levels (p=0.005), whereas HbA1c levels were associated with T allele of CYP19A1 in the CAD group (p=0.004) and male CAD group (p=0.018).
Conclusion: The CYP19A1 and ESR1 polymorphisms were associated with the presence and severity of CAD. These gene polymorphisms warrant further studies for the elucidation of their contribution to CAD development.
Anahtar Kelime: Cinsiyet hormonu genlerindeki polimorfizmlerin koroner arter hastalığı ve ciddiyeti ile ilişkisi
Öz: Amaç: Koroner arter hastalığı (KAH) dünyada önemli bir halk sağlığı sorunudur. Hastalığın etiyolojisinin altında yatan klasik risk faktörleri tanımlanmış olsa da bu faktörleri etkileyen moleküler mekanizmalar tamamen aydınlatılamamıştır. Bu nedenle, hastalığın gelişimi ve tedavisinde rol oynayabilecek aday gen polimorfizmlerinin tanımlanması önem taşımaktadır. Bu bilgiler ışığında, bu çalışmada ateroskleroz ile ilişkili iki gen olan ESR1 ve CYP19A1 gen polimorfizmleri araştırılmıştır.
Yöntemler: 339 KAH ve KAH olmayan bireye ait periferik kan örneğinden DNA izolasyonu yapılmış ve çalışmaya katılan bireyler CYP19A1 rs10046 (C/T) ve ESR1 rs2175898 (A/G) polimorfizmleri için hibridizasyon probları kullanılarak genotiplenmiştir. Kan örnekleri koroner anjiyografiden önce alınarak biyokimyasal analizler yapılmıştır. Bunun yanında, çalışmaya katılan bireylerin koroner anjiyografi sonuçlarına göre Gensini ve SYNTAX skorları belirlenmiştir.
Bulgular: ESR1 polimorfizmi G allel taşıyıcılığı erkeklerde KAH ile ilişkili bulunmuştur (p=0.036). Yaş, HDL-K, LDL-K düzeyleri ve sigara içme durumuna göre ayarlama yapılan lojistik regresyon analizinde, ESR1 G allel taşıyıcılığının erkeklerde KAH ile ilişkili olduğu gösterilmiştir (OR=2.12, [%95 GA 1.01–4.1] p=0.025). Yapılan lojistik regresyon analizlerinde, CYP19A1 rs10046 T allel taşıyıcısı bireylerde kompleks KAH riskinin 2.84 kat arttığı görülmüştür (p=0.016). Buna ek olarak, CYP19A1 rs10046 T alel taşıyıcılığı, SYNTAX ve Gensini skorları ile ilişkili bulunmuştur (p<0.05). ESR1 G allel taşıyıcılığı kadın hastalarda yüksek adiponektin düzeyi (p=0.005) ile ilişkili bulunurken CYP19A1 T alleli, HbA1c düzeyleri ile KAH hastalarında (p=0.004) ve erkek KAH hastalarında (p=0.018) ilişkili bulunmuştur.
Sonuç: CYP19A1 ve ESR1 polimorfizmleri KAH şiddeti ve KAH varlığı ile ilişkili bulunmuştur. Çalışılan gen polimorfizmlerin KAH gelişimindeki etkilerinin belirlenebilmesi için daha çok çalışmaya ihtiyaç bulunmaktadır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | coban n, Ozuynuk-Ertugrul A, Erkan A, KAYA A, Ekici B, Guclu-Geyik F, Ademoglu E, CAN G, Erginel-Unaltuna N (2022). Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease. , 22 - 33. 10.5543/tkda.2022.17203 |
| Chicago | coban neslihan,Ozuynuk-Ertugrul Aybike Sena,Erkan Aycan Fahri,KAYA AYŞEM,Ekici Berkay,Guclu-Geyik Filiz,Ademoglu Evin,CAN GÜNAY,Erginel-Unaltuna Nihan Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease. (2022): 22 - 33. 10.5543/tkda.2022.17203 |
| MLA | coban neslihan,Ozuynuk-Ertugrul Aybike Sena,Erkan Aycan Fahri,KAYA AYŞEM,Ekici Berkay,Guclu-Geyik Filiz,Ademoglu Evin,CAN GÜNAY,Erginel-Unaltuna Nihan Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease. , 2022, ss.22 - 33. 10.5543/tkda.2022.17203 |
| AMA | coban n,Ozuynuk-Ertugrul A,Erkan A,KAYA A,Ekici B,Guclu-Geyik F,Ademoglu E,CAN G,Erginel-Unaltuna N Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease. . 2022; 22 - 33. 10.5543/tkda.2022.17203 |
| Vancouver | coban n,Ozuynuk-Ertugrul A,Erkan A,KAYA A,Ekici B,Guclu-Geyik F,Ademoglu E,CAN G,Erginel-Unaltuna N Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease. . 2022; 22 - 33. 10.5543/tkda.2022.17203 |
| IEEE | coban n,Ozuynuk-Ertugrul A,Erkan A,KAYA A,Ekici B,Guclu-Geyik F,Ademoglu E,CAN G,Erginel-Unaltuna N "Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease." , ss.22 - 33, 2022. 10.5543/tkda.2022.17203 |
| ISNAD | coban, neslihan vd. "Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease". (2022), 22-33. https://doi.org/10.5543/tkda.2022.17203 |
| APA | coban n, Ozuynuk-Ertugrul A, Erkan A, KAYA A, Ekici B, Guclu-Geyik F, Ademoglu E, CAN G, Erginel-Unaltuna N (2022). Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease. Türk Kardiyoloji Derneği Arşivi, 50(1), 22 - 33. 10.5543/tkda.2022.17203 |
| Chicago | coban neslihan,Ozuynuk-Ertugrul Aybike Sena,Erkan Aycan Fahri,KAYA AYŞEM,Ekici Berkay,Guclu-Geyik Filiz,Ademoglu Evin,CAN GÜNAY,Erginel-Unaltuna Nihan Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease. Türk Kardiyoloji Derneği Arşivi 50, no.1 (2022): 22 - 33. 10.5543/tkda.2022.17203 |
| MLA | coban neslihan,Ozuynuk-Ertugrul Aybike Sena,Erkan Aycan Fahri,KAYA AYŞEM,Ekici Berkay,Guclu-Geyik Filiz,Ademoglu Evin,CAN GÜNAY,Erginel-Unaltuna Nihan Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease. Türk Kardiyoloji Derneği Arşivi, vol.50, no.1, 2022, ss.22 - 33. 10.5543/tkda.2022.17203 |
| AMA | coban n,Ozuynuk-Ertugrul A,Erkan A,KAYA A,Ekici B,Guclu-Geyik F,Ademoglu E,CAN G,Erginel-Unaltuna N Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease. Türk Kardiyoloji Derneği Arşivi. 2022; 50(1): 22 - 33. 10.5543/tkda.2022.17203 |
| Vancouver | coban n,Ozuynuk-Ertugrul A,Erkan A,KAYA A,Ekici B,Guclu-Geyik F,Ademoglu E,CAN G,Erginel-Unaltuna N Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease. Türk Kardiyoloji Derneği Arşivi. 2022; 50(1): 22 - 33. 10.5543/tkda.2022.17203 |
| IEEE | coban n,Ozuynuk-Ertugrul A,Erkan A,KAYA A,Ekici B,Guclu-Geyik F,Ademoglu E,CAN G,Erginel-Unaltuna N "Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease." Türk Kardiyoloji Derneği Arşivi, 50, ss.22 - 33, 2022. 10.5543/tkda.2022.17203 |
| ISNAD | coban, neslihan vd. "Association of polymorphisms in the sex hormone genes with the presence and severity of coronary artery disease". Türk Kardiyoloji Derneği Arşivi 50/1 (2022), 22-33. https://doi.org/10.5543/tkda.2022.17203 |
