Evaluation of Oxidative Stress, Immune System and Mineral 
Concentrations in Milk and Serum of Cows with Clinical and 
Subclinical Mastitis Naturally Infected by Staphylococcus aureus

Demir, Pinar; Eski, Funda; mis, leyla; kurt, serdal

doi:10.9775/kvfd.2021.26281

Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus

Serdal KURT, (Kahramanmaraş İstiklal Üniversitesi, Elbistan Meslek Yüksekokulu, Veterinerlik Bölümü, Kahramanmaraş, Türkiye)

Funda EŞKİ, (Çukurova Üniversitesi, Ceyhan Veteriner Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Adana, Türkiye)

Leyla MİS, (Van Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Veteriner Fizyoloji Anabilim Dalı, Van, Türkiye)

Pınar AYVAZOĞLU DEMİR (Kafkas Üniversitesi, Veteriner Fakültesi, Hayvancılık Ekonomisi ve Yönetimi Anabilim Dalı, Kars, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

2 0

Yıl: 2021 Cilt: 27 Sayı: 6 Sayfa Aralığı: 755 - 762 Metin Dili: İngilizce DOI: 10.9775/kvfd.2021.26281 İndeks Tarihi: 22-06-2022

Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus

Öz:

The aim of this study was to investigate effect of Staphylococcus aureus on oxidative stress status (TAS, TOS, OSI), immune system (IL-1β, IL-6, TNF-α), and mineral (Mg, Fe, Zn, Cu, Na and Ca) concentrations in milk and serum of cows with mastitis. The cows were allocated to three groups according to mammary health status as follows: healthy (Group 1), clinical (Group 2) and subclinical mastitis cows (Group 3). IL-1β levels in serum and milk increased in Group 2 compared to Group 1 and Group 3 (P<0.001; P<0.05). Milk IL-6 level was greater in Group 3 and Group 2 than in Group 1 (P<0.01). Blood TNF-α (P<0.001), TOS and OSI levels (P<0.01) were higher in Group 2 than other groups. Milk TNF-α level increased and blood TAS level decreased in Group 2 compared to Group 1 (P<0.05). Milk TOS (P<0.01) and OSI (P<0.05) levels increased in Group 3 compared to Group 1. Blood and milk Mg levels increased in Group 1 (P<0.05) and Group 2 (P<0.001), respectively. Milk Fe (P<0.01) and Na levels were greater in Group 2 (P<0.001). Blood Zn level was lower in Group 2 compared to Group 3 (P<0.05). While blood Cu level decreased in Group 1 compared to other groups, milk Cu level decreased in Group 3 compared to other groups (P<0.001). In conclusion, S. aureus had significant effects on oxidative stress, cytokine and mineral levels in milk and blood serum of cows with clinical and subclinical mastitis. However, since there were specific changes only in milk OSI and IL-6 levels according to other parameters, it is thought that milk IL-6 and OSI levels may be a diagnostic tool in the detection of subclinical mastitis.

Anahtar Kelime:

Staphylococcus aureus İle Doğal Olarak Enfekte Olan Klinik veSubklinik Mastitisli İneklerin Süt ve Serumlarında Oksidatif Stres,Bağışıklık Sistemi ve Mineral Konsantrasyonlarının Değerlendirilmesi

Öz:

Bu çalışmanın amacı, Staphylococcus aureus’un mastitisli ineklerin süt ve kan serumlarında oksidatif stres durumu (TAS, TOS, OSI), bağışıklık sistemi (IL1β, IL-6, TNF-α) ve mineral (Mg, Fe, Zn, Cu, Na ve Ca) konsantrasyonları üzerine etkisini araştırmaktı. Çalışma sağlıklı (Grup 1), klinik (Grup 2) ve subklinik mastitisli (Grup 3) inekler olmak üzere üç grup üzerinde yürütüldü. Grup 1 ve Grup 3’e göre Grup 2’de kan ve sütteki IL-1β düzeyleri arttı (P<0.001; P<0.05). Grup 1’e göre Grup 3 ve Grup 2’de süt IL-6 düzeyi daha yüksekti (P<0.01). Diğer gruplara göre Grup 2’de kan TNF-α (P<0.001), TOS ve OSI düzeyleri (P<0.01) daha yüksekti. Grup 1’e göre Grup 2 de süt TNF-α düzeyi yükseldi ve kan TAS düzeyi azaldı (P<0.05). Grup 1’e kıyasla Grup 3’te süt TOS (P<0.01) ve OSI (P<0.05) düzeyleri yükseliş gösterdi. Diğer gruplara göre kan ve süt Mg düzeyleri sırasıyla Grup 1 (P<0.05) ve Grup 2’de (P<0.001) arttı. Diğer gruplara göre Grup 2’de süt Fe (P<0.01) ve Na düzeyleri arttı (P<0.001). Grup 3’e göre Grup 2’de kan Zn düzeyi düşüş gösterdi (P<0.05). Kan Cu düzeyi diğer gruplara göre Grup 1’de düşerken, süt Cu düzeyi diğer gruplara kıyasla Grup 3’te azaldı (P<0.001). Sonuç olarak, S. aureus’un klinik ve subklinik mastitisli ineklerin süt ve kan serumlarında oksidatif stres, sitokin ve mineral seviyeleri üzerinde önemli etkileri olduğu görülmüştür. Ancak diğer parametrelere göre sadece süt OSI ve IL-6 düzeylerinde spesifik değişiklikler olduğu için süt IL-6 ve OSI düzeylerinin subklinik mastitisin saptanmasında tanı aracı olabileceği düşünülmektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	kurt s, Eski F, mis l, Demir P (2021). Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus. , 755 - 762. 10.9775/kvfd.2021.26281
Chicago	kurt serdal,Eski Funda,mis leyla,Demir Pinar Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus. (2021): 755 - 762. 10.9775/kvfd.2021.26281
MLA	kurt serdal,Eski Funda,mis leyla,Demir Pinar Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus. , 2021, ss.755 - 762. 10.9775/kvfd.2021.26281
AMA	kurt s,Eski F,mis l,Demir P Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus. . 2021; 755 - 762. 10.9775/kvfd.2021.26281
Vancouver	kurt s,Eski F,mis l,Demir P Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus. . 2021; 755 - 762. 10.9775/kvfd.2021.26281
IEEE	kurt s,Eski F,mis l,Demir P "Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus." , ss.755 - 762, 2021. 10.9775/kvfd.2021.26281
ISNAD	kurt, serdal vd. "Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus". (2021), 755-762. https://doi.org/10.9775/kvfd.2021.26281

APA	kurt s, Eski F, mis l, Demir P (2021). Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(6), 755 - 762. 10.9775/kvfd.2021.26281
Chicago	kurt serdal,Eski Funda,mis leyla,Demir Pinar Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.6 (2021): 755 - 762. 10.9775/kvfd.2021.26281
MLA	kurt serdal,Eski Funda,mis leyla,Demir Pinar Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.6, 2021, ss.755 - 762. 10.9775/kvfd.2021.26281
AMA	kurt s,Eski F,mis l,Demir P Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(6): 755 - 762. 10.9775/kvfd.2021.26281
Vancouver	kurt s,Eski F,mis l,Demir P Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(6): 755 - 762. 10.9775/kvfd.2021.26281
IEEE	kurt s,Eski F,mis l,Demir P "Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.755 - 762, 2021. 10.9775/kvfd.2021.26281
ISNAD	kurt, serdal vd. "Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/6 (2021), 755-762. https://doi.org/10.9775/kvfd.2021.26281