Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells
Yıl: 2022 Cilt: 12 Sayı: 1 Sayfa Aralığı: 6 - 11 Metin Dili: İngilizce DOI: 10.26650/experimed.2021.1038579 İndeks Tarihi: 22-06-2022
Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells
Öz: Objective: In recent years, especially combined treatment options that will reduce the side effects of drugs have attracted attention.
Carmofur is a new potent agent being investigated in the treatment of cancer. This study aimed to investigate the cytotoxic and apoptotic
effects of carmofur+vitamin C combination in colon cancer cells.
Materials and Methods: Carmofur in the range of 7.8-250 µM, vitamin C in 7.8 µM-2mM and carmofur+vitamin C in different concentrations
were evaluated on HCT116 cells by MTT test. In addition, the cell death pathway was determined by an apoptosis-necrosis assay.
Results: The IC50 value of carmofur in HCT116 cells was 8 µM, vitamin C was not effective at low doses, and the IC50 value was 2.2 mM.
When carmofur+vitamin C were applied to HCT116 cells, 4 µM carmofur and 125 µM vitamin C together inhibited half of the cells (IC50),
while 4 µM carmofur and 2 mM vitamin C inhibited half of the cells similarly.
Conclusion: Vitamin C doubled the anticancer effect of carmofur. As a result, it was observed that vitamin C supplementation significantly
increased cell death in anticancer drug administration. In addition, it was determined that the addition of vitamin C significantly increased
the apoptotic activity.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Danışman F (2022). Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells. , 6 - 11. 10.26650/experimed.2021.1038579 |
| Chicago | Danışman Ferdane Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells. (2022): 6 - 11. 10.26650/experimed.2021.1038579 |
| MLA | Danışman Ferdane Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells. , 2022, ss.6 - 11. 10.26650/experimed.2021.1038579 |
| AMA | Danışman F Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells. . 2022; 6 - 11. 10.26650/experimed.2021.1038579 |
| Vancouver | Danışman F Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells. . 2022; 6 - 11. 10.26650/experimed.2021.1038579 |
| IEEE | Danışman F "Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells." , ss.6 - 11, 2022. 10.26650/experimed.2021.1038579 |
| ISNAD | Danışman, Ferdane. "Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells". (2022), 6-11. https://doi.org/10.26650/experimed.2021.1038579 |
| APA | Danışman F (2022). Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells. EXPERIMED, 12(1), 6 - 11. 10.26650/experimed.2021.1038579 |
| Chicago | Danışman Ferdane Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells. EXPERIMED 12, no.1 (2022): 6 - 11. 10.26650/experimed.2021.1038579 |
| MLA | Danışman Ferdane Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells. EXPERIMED, vol.12, no.1, 2022, ss.6 - 11. 10.26650/experimed.2021.1038579 |
| AMA | Danışman F Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells. EXPERIMED. 2022; 12(1): 6 - 11. 10.26650/experimed.2021.1038579 |
| Vancouver | Danışman F Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells. EXPERIMED. 2022; 12(1): 6 - 11. 10.26650/experimed.2021.1038579 |
| IEEE | Danışman F "Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells." EXPERIMED, 12, ss.6 - 11, 2022. 10.26650/experimed.2021.1038579 |
| ISNAD | Danışman, Ferdane. "Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells". EXPERIMED 12/1 (2022), 6-11. https://doi.org/10.26650/experimed.2021.1038579 |
