Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced
acute seizures in mice through GABA and glutamate mediated mechanism

KARABULUT, SEBAHATTIN; ŞAHİN, Bilal; AKKAYA, Recep

doi:10.30714/j-ebr.2022173850

Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism

Bilal SAHIN, (Sivas Cumhuriyet Üniversitesi Tıp Fakültesi, Fizyoloji Anabilim Dalı, Sivas, Türkiye)

Recep AKKAYA, (Sivas Cumhuriyet Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Sivas, Türkiye)

Sebahattin KARABULUT (Sivas Cumhuriyet Üniversitesi Sağlık Hizmetleri Meslek Yüksekokulu Tıbbi Hizmetler ve Teknikleri Anabilim Dalı, Sivas, Türkiye)

Experimental Biomedical Research

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Yıl: 2022 Cilt: 5 Sayı: 1 Sayfa Aralığı: 48 - 57 Metin Dili: İngilizce DOI: 10.30714/j-ebr.2022173850 İndeks Tarihi: 23-06-2022

Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism

Öz:

Aim: To investigate the possible anticonvulsive effect of the selective COX-2 inhibitor meloxicam in pentylenetetrazole (PTZ)-induced epileptic seizures in mice and to examine its possible role on inhibition and excitation balance in the brain. Method: 30 BALB-c albino mice (16-18 weeks old) weighing 30-33 gr were used. Animals were randomly divided into five groups (n = 6 for each group). Group 1: control, group 2: received saline (10 ml/kg, i.p.) 30 minutes before PTZ (60 mg/kg i.p.) administration, group 3: received saline (10 ml/kg, i.p.) 30 minutes after PTZ (60 mg/kg i.p.) injection, group 4: received 60 mg/kg meloxicam i.p., 30 minutes before PTZ (60 mg/kg i.p.) administration. Group 5: received meloxicam (60 mg/kg i.p.) 30 minutes after PTZ injection (60 mg/kg, i.p.). The animals were observed for 30 minutes and the seizure stages and first myoclonic jerk times (FMJ) were recorded. After 24 hours, brain tissues were removed and the cortex and hippocampus were separated for biochemical assessments. ELISA method was used to measure GABA and glutamate levels. Results: Administration of meloxicam before PTZ induced seizure, reduced seizure stages and prolonged FMJ duration (p<0.05). Pre-treatment with meloxicam increased GABA levels in the cortex and decreased glutamate levels in the hippocampus (p<0.05). Post-treatment of meloxicam after PTZ-induced seizure increased GABA levels in the hippocampus (p<0.05). Conclusion: The results of our experimental study suggest that meloxicam has anti-convulsive effects and these effects may be mediated by GABA and glutamate, which are the main indicators of inhibition and excitation balance in the brain.

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APA	ŞAHİN B, AKKAYA R, KARABULUT S (2022). Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. , 48 - 57. 10.30714/j-ebr.2022173850
Chicago	ŞAHİN Bilal,AKKAYA Recep,KARABULUT SEBAHATTIN Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. (2022): 48 - 57. 10.30714/j-ebr.2022173850
MLA	ŞAHİN Bilal,AKKAYA Recep,KARABULUT SEBAHATTIN Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. , 2022, ss.48 - 57. 10.30714/j-ebr.2022173850
AMA	ŞAHİN B,AKKAYA R,KARABULUT S Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. . 2022; 48 - 57. 10.30714/j-ebr.2022173850
Vancouver	ŞAHİN B,AKKAYA R,KARABULUT S Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. . 2022; 48 - 57. 10.30714/j-ebr.2022173850
IEEE	ŞAHİN B,AKKAYA R,KARABULUT S "Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism." , ss.48 - 57, 2022. 10.30714/j-ebr.2022173850
ISNAD	ŞAHİN, Bilal vd. "Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism". (2022), 48-57. https://doi.org/10.30714/j-ebr.2022173850

APA	ŞAHİN B, AKKAYA R, KARABULUT S (2022). Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. Experimental Biomedical Research, 5(1), 48 - 57. 10.30714/j-ebr.2022173850
Chicago	ŞAHİN Bilal,AKKAYA Recep,KARABULUT SEBAHATTIN Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. Experimental Biomedical Research 5, no.1 (2022): 48 - 57. 10.30714/j-ebr.2022173850
MLA	ŞAHİN Bilal,AKKAYA Recep,KARABULUT SEBAHATTIN Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. Experimental Biomedical Research, vol.5, no.1, 2022, ss.48 - 57. 10.30714/j-ebr.2022173850
AMA	ŞAHİN B,AKKAYA R,KARABULUT S Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. Experimental Biomedical Research. 2022; 5(1): 48 - 57. 10.30714/j-ebr.2022173850
Vancouver	ŞAHİN B,AKKAYA R,KARABULUT S Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. Experimental Biomedical Research. 2022; 5(1): 48 - 57. 10.30714/j-ebr.2022173850
IEEE	ŞAHİN B,AKKAYA R,KARABULUT S "Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism." Experimental Biomedical Research, 5, ss.48 - 57, 2022. 10.30714/j-ebr.2022173850
ISNAD	ŞAHİN, Bilal vd. "Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism". Experimental Biomedical Research 5/1 (2022), 48-57. https://doi.org/10.30714/j-ebr.2022173850