Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy
Yıl: 2022 Cilt: 47 Sayı: 2 Sayfa Aralığı: 765 - 775 Metin Dili: İngilizce DOI: 10.17826/cumj.1090930 İndeks Tarihi: 29-07-2022
Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy
Öz: Purpose: The aim of this research is to see how Melissa officinalis L. affects the death pathways of MCF-7 breast cancer cells in vitro.
Materials and Methods: In order to determine the cytotoxic activity and IC50 value of the 70% methanolic extract prepared from the aerial parts of Melissa officinalis, on the MCF-7 breast cancer cell line; the extract was applied at different concentrations and times and MTT analysis was performed. Apoptotic effects of Melissa officinalis on breast cancer cells at the determined IC50 dose were revealed by TUNEL method, and autophagic effects were revealed using Beclin-1 and LC3 immunofluorescence staining method.
Results: When the effects of Melissa officinalis on MCF-7 breast cancer cells were evaluated, it was observed that the cell viability of the MCF-7 breast cancer cell line was decreased in a dose- and time-dependent manner. In addition, it was observed that it induced apoptosis and increased the immunoreactivity of autophagy markers Beclin-1 and LC3.
Conclusion: Findings from the study showed that Melissa officinalis has a cytotoxic effect on MCF-7 breast cancer cells through both apoptosis and autophagy cell death pathways. These data will lead to further in vitro studies necessary to elucidate the antitumor mechanism of action.
Anahtar Kelime: Melissa officinalis L. bitkisinin apoptoz ve otofaji yoluyla insan meme kanser hücre hattı üzerine etkisi
Öz: Amaç: Bu araştırmanın amacı, Melissa officinalis L. bitkisinin in vitro olarak MCF-7 meme kanseri hücrelerindeki ölüm yollarını nasıl etkilediğini görmektir.
Gereç ve Yöntem: Melissa officinalis bitkisinin topraküstü kısımlarından hazırlanan %70 metanolik ekstrenin sitotoksik aktivitesi ve IC50 değerini belirlemek için MCF-7 meme kanseri hücre hattı farklı konsantrasyonlar da ve sürelerde Melissa officinalis’ e maruz bırakılarak MTT analizi yapıldı. Melissa officinalis' in belirlenen IC50 dozunda meme kanseri hücreleri üzerindeki apoptotik etkileri TUNEL yöntemi ile, otofajik etkileri Beclin-1 ve LC3 immünofloresan boyama yöntemi kullanılarak ortaya çıkarıldı.
Bulgular: Melissa officinalis’ in, MCF-7 meme kanseri hücreleri üzerindeki etkileri değerlendirildiğinde, MCF-7 meme kanseri hücre hattındaki hücre canlılığını doza ve zamana bağlı bir şekilde azalttığı görüldü. Bunun yanı sıra, apopitozu indüklediği ve otofaji belirteçleri olan Beclin-1 ve LC3 immunreaktivitesini de artırdığı gözlendi.
Sonuç: Çalışmadan elde edilen bulgular, Melissa officinalis’in hem apoptoz hem de otofaji hücre ölümü yolları üzerinden MCF-7 meme kanseri hücreleri üzerinde sitotoksik bir etkiye sahip olduğunu göstermiştir. Bu veriler, antitümör etki mekanizmasını aydınlatmak için gerekli olan daha ileri in vitro çalışmalara öncülük edecektir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Akkuzu MZ, Küçüköner M, Irtegun S, Akdeniz N, Urakçı Z, Kaplan MA et al. Meme kanserinde Brca-1 ve Brca-2’ de sık görülen polimorfizm mutasyonların bölgemizde varlığı. Dicle Med J. 2019;46:623-31.
- 2. Kampa M, Nifli AP, Notas G, Castanas E. Polyphenols and cancer cell growth. Rev Physiol Biochem Pharmacol. 2007;159:79-113.
- 3. Sonnenblick A, Fumagalli D, Sotiriou C, Piccart M. Is the differentiation into molecular subtypes of breast cancer important for staging, local and systemic therapy, and follow up? Cancer Treat Rev. 2014;40:1089-95.
- 4. Eliyatkin N, Yalcin E, Zengel B, Aktaş S, Vardar E. Molecular classification of breast carcinoma: from traditional, old-fashioned way to a new age, and a new way. J Breast Heal. 2015;11:59-66.
- 5. Comsa S, Cîmpean AM, Raica M. The story of MCF7 breast cancer cell line: 40 Years of experience in research. Anticancer Res. 2015;35:3147-54.
- 6. Sweeney EE, McDaniel RE, Maximov PY, Fan P, Craig Jordan V. Models and mechanisms of acquired antihormone resistance in breast cancer: Significant clinical progress despite limitations. Horm Mol Biol Clin Investig. 2012;9:143-63.
- 7. Baskal G, Köngül E, Karatoprak GS. Traditional Usage of Melissa officinalis. J Health Sci. 2017;26:267- 69.
- 8. Gurbuz P, Martinez A, Perez C, Martinez-Gonzalez L, Goger F, Ayran I. Potential anti-Alzheimer effects of selected Lamiaceae plants through polypharmacology on glycogen synthase kinase-3β, βsecretase, and casein kinase 1δ. Ind Crops Prod. 2019;138:111431.
- 9. Saraydin SU, Tuncer E, Tepe B, Karadayi S, Özer H, Şen M et al. Antitumoral effects of melissa officinalis on breast cancer in vitro and in vivo. Asian Pac J Cancer Prev. 2012;13:2765-70.
- 10. Weidner C, Rousseau M, Plauth A, Wowro SJ, Fischer C, Abdel-Aziz H et al. Melissa officinalis extract induces apoptosis and inhibits proliferation in colon cancer cells through formation of reactive oxygen species. Phytomedicine. 2015;22:262-70.
- 11. Encalada MA, Hoyos KM, Rehecho S, Berasategi I, García-Íñiguez de Ciriano M, Ansorena D et al. Antiproliferative effect of Melissa officinalis on Human Colon Cancer Cell Line. Plant Foods Hum Nutr. 2011;66:328-34.
- 12. Jahanban-Esfahlan A, Modaeinama S, Abasi M, Mesgari Abbasi M, Jahanban-Esfahlan R. Anti proliferative properties of Melissa officinalis in different human cancer cells. Asian Pac J Cancer Prev. 2015;16:5703-7.
- 13. Matsunaga K, Saitoh T, Tabata K, Omori H, Satoh T, Kurotori N et al. Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages. Nat Cell Biol. 2009;11:385-96.
- 14. Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Arozena AA et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 2016;12:1-222.
- 15. Magalhães DB, Castro I, Lopes-Rodrigues V, Pereira JM, Barros L, Ferreira ICFR et al. Melissa officinalis L. ethanolic extract inhibits the growth of a lung cancer cell line by interfering with the cell cycle and inducing apoptosis. Food Funct. 2018;9:3134-42.
- 16. Ghiulai R, Avram S, Stoian D, Pavel IZ, Coricovac D, Oprean C et al. Lemon balm extracts prevent breast cancer progression in vitro and in ovo on chorioallantoic membrane assay. Evid Based Complement Alternat Med. 2020;2020:6489159.
- 17. Mosmann T. Rapid colorimetric assay for celluler growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55- 63.
- 18. Kumari S, Badana AK, Mohan GM, Naik GS, Malla RR. Synergistic effects of coralyne and paclitaxel on cell migration and proliferation of breast cancer cells lines. Biomed Pharmacother. 2017;91:436-45.
- 19. Maryam R, Faegheh S, Majid AS, Kazem NK. Effect of quercetin on secretion and gene expression of leptin in breast cancer. J Tradit Chin Med. 2017;37:321-5.
- 20. Dias MI, Barros L, Sousa MJ, Ferreira IC. Systematic comparison of nutraceuticals and antioxidant potential of cultivated, in vitro cultured and commercial Melissa officinalis samples. Food Chem Toxicol. 2012;50:1866-73.
- 21. Jahanban-Esfahlan R, Seidi K, Monfaredan A. The herbal medicine Melissa officinalis extract effects on gene expression of p53, Bcl-2, Her2, VEGF-A and hTERT in human lung, breast and prostate cancer cell lines.Gene. 2017;613:14-9.
- 22. Balunas MJ, Su B, Brueggemeier RW. Natural products as aromatase inhibitors. Anticancer Agents Med Chem. 2008;8:646-82.
- 23. Yesil-Celiktas O, Sevimli C, Bedir E, Vardar-Sukan F. Inhibitory effects of rosemary extracts, carnosic acid and rosmarinic acid on the growth of various human cancer cell lines. Plant Foods Hum Nutr. 2010;65:158-63.
- 24. Katary MA, Abdelsayed R, Alhashim A, Abdelhasib M, Elmarakby AA. Salvianolic acid b slows the progression of breast cancer cell growth via enhancement of apoptosis and reduction of oxidative stress, inflammation, and angiogenesis. Int J Mol Sci. 2019;20:5653.
- 25. Quan G, Chen W, Wen Y, Yan Y, Gu M, Pan Y. Chemoprotective efficacy of salvianolic acid B via triggering apoptosis in MCF-7 human breast cancer cells. Int J Pharm. 2019;15:110-5.
| APA | Bitgen N, BARAN M, Önder G, Suna P, Gürbüz P, Yay A (2022). Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy. , 765 - 775. 10.17826/cumj.1090930 |
| Chicago | Bitgen Nazmiye,BARAN MÜNEVVER,Önder Gözde Özge,Suna Pınar,Gürbüz Perihan,Yay Arzu Hanım Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy. (2022): 765 - 775. 10.17826/cumj.1090930 |
| MLA | Bitgen Nazmiye,BARAN MÜNEVVER,Önder Gözde Özge,Suna Pınar,Gürbüz Perihan,Yay Arzu Hanım Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy. , 2022, ss.765 - 775. 10.17826/cumj.1090930 |
| AMA | Bitgen N,BARAN M,Önder G,Suna P,Gürbüz P,Yay A Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy. . 2022; 765 - 775. 10.17826/cumj.1090930 |
| Vancouver | Bitgen N,BARAN M,Önder G,Suna P,Gürbüz P,Yay A Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy. . 2022; 765 - 775. 10.17826/cumj.1090930 |
| IEEE | Bitgen N,BARAN M,Önder G,Suna P,Gürbüz P,Yay A "Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy." , ss.765 - 775, 2022. 10.17826/cumj.1090930 |
| ISNAD | Bitgen, Nazmiye vd. "Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy". (2022), 765-775. https://doi.org/10.17826/cumj.1090930 |
| APA | Bitgen N, BARAN M, Önder G, Suna P, Gürbüz P, Yay A (2022). Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy. Cukurova Medical Journal, 47(2), 765 - 775. 10.17826/cumj.1090930 |
| Chicago | Bitgen Nazmiye,BARAN MÜNEVVER,Önder Gözde Özge,Suna Pınar,Gürbüz Perihan,Yay Arzu Hanım Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy. Cukurova Medical Journal 47, no.2 (2022): 765 - 775. 10.17826/cumj.1090930 |
| MLA | Bitgen Nazmiye,BARAN MÜNEVVER,Önder Gözde Özge,Suna Pınar,Gürbüz Perihan,Yay Arzu Hanım Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy. Cukurova Medical Journal, vol.47, no.2, 2022, ss.765 - 775. 10.17826/cumj.1090930 |
| AMA | Bitgen N,BARAN M,Önder G,Suna P,Gürbüz P,Yay A Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy. Cukurova Medical Journal. 2022; 47(2): 765 - 775. 10.17826/cumj.1090930 |
| Vancouver | Bitgen N,BARAN M,Önder G,Suna P,Gürbüz P,Yay A Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy. Cukurova Medical Journal. 2022; 47(2): 765 - 775. 10.17826/cumj.1090930 |
| IEEE | Bitgen N,BARAN M,Önder G,Suna P,Gürbüz P,Yay A "Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy." Cukurova Medical Journal, 47, ss.765 - 775, 2022. 10.17826/cumj.1090930 |
| ISNAD | Bitgen, Nazmiye vd. "Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy". Cukurova Medical Journal 47/2 (2022), 765-775. https://doi.org/10.17826/cumj.1090930 |
