Analysis of Changes in Serum Levels and Gene
Expression Profiles of Novel Adipocytokines
(Omentin, Vaspin, Irisin and Visfatin) and Their
Correlation with Serum C-reactive Protein Levels
in Women Diagnosed with Endometriosis

SEZGİNER, Ecem KAYA; GÜR, Serap; Topçu, Hasan Onur; KIRLANGIÇ, Ömer Faruk; TANRIVERDİ, Merve Didem EŞKİN

doi:10.4274/tjps.galenos.2021.52284

Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis

Ecem KAYA SEZGİNER, (Ankara Üniversitesi Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye)

Ömer Faruk KIRLANGIÇ, (Türkiye Sağlık Bilimleri Üniversitesi, Gülhane Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Ankara, Türkiye)

Merve Didem EŞKİN TANRIVERDİ, (Türkiye Sağlık Bilimleri Üniversitesi, Ankara Şehir Hastanesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Ankara, Türkiye)

Hasan Onur TOPÇU, (Memorial Ankara Hastanesi, Kadın Hastalıkları ve Doğum Kliniği, Ankara, Türkiye)

Serap GÜR (Ankara Üniversitesi Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, Ankara, Türkiye)

Turkish Journal of Pharmaceutical Sciences

10 0

Yıl: 2022 Cilt: 19 Sayı: 1 Sayfa Aralığı: 48 - 53 Metin Dili: İngilizce DOI: 10.4274/tjps.galenos.2021.52284 İndeks Tarihi: 26-06-2022

Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis

Öz:

Objectives: This study aimed to investigate the role of new adipocytokines (omentin, vaspin, irisin and visfatin) in the development of endometriosis and the relationship of these adipocytokines with the inflammatory marker, C-reactive protein (CRP) levels in serum. Materials and Methods: In this study, endometriosis (n=16) and control groups (n=14) were determined via ultrasound. Serum omentin, vaspin and irisin levels were measured by ELISA method. CRP levels in serum and the gene expression of visfatin and vaspin in whole blood samples were determined by clinical analyzer and the real-time polymerase chain reaction, respectively. Results: Serum irisin and CRP levels in the endometriosis group were significantly higher than in the control group. Irisin protein levels demonstrated a positive correlation with body mass index and CRP in women diagnosed with endometriosis. No statistically significant difference was found in serum omentin and vaspin levels between groups. The visfatin and vaspin gene expression in whole blood samples from the endometriosis group was found to be significantly lower than the control group. Conclusion: Increased levels of serum irisin and decreased visfatin and vaspin gene expressions in blood may be considered as a potential biomarker in endometriosis. The identification of new adipocytokines, which demonstrate an alteration in the presence of endometriosis and the relationship between these adipocytokines and inflammation will facilitate the detection of mechanisms involved in endometriosis and will lead to the development of targeted therapy.

Anahtar Kelime:

Endometriozis Tanısı Alan Kadınlarda Yeni Adipositokinlerin (Omentin, Vaspin, İrisin ve Visfatin) Serum Seviyelerindeki ve Gen Ekspresyon Profillerindeki Değişimlerinin Analizi ve Bu Adipositokinlerin Serum C-reaktif Protein Düzeyleri ile Korelasyonu

Öz:

Amaç: Bu çalışmanın amacı, yeni adipositokinlerin (omentin, vaspin, irisin ve visfatin) endometriozis gelişimindeki rolünü ve bu adipositokinlerin inflamatuvar belirteç olan serumdaki C-reaktif protein (CRP) düzeyi ile ilişkisini araştırmaktır Gereç ve Yöntemler: Bu çalışmada endometriozis (n=16) ve kontrol grupları (n=14) ultrason aracılığı ile belirlenmiştir. Serum omentin, vaspin ve irisin seviyeleri ELISA yöntemi ile ölçülmüştür. Serumda CRP seviyeleri ile tam kan örneklerinde visfatin ve vaspin gen ekspresyonu sırası ile klinik analiz cihazı ve gerçek zamanlı-polimeraz zincir reaksiyonu ile belirlenmiştir. Bulgular: Endometriozis grubunda, serum irisin ve CRP seviyeleri kontrol grubuna göre anlamlı olarak yüksek bulunmuştur. Endometriozis tanısı alan kadınlarda irisin protein düzeyleri, vücut kitle indeksi ve CRP ile pozitif korelasyon göstermiştir. Serum omentin ve vaspin düzeylerinde gruplar arasında istatistiksel olarak anlamlı fark bulunmamıştır. Endometriozis grubundan alınan tam kan örneklerinde, visfatin ve vaspin gen ekspresyonu, kontrol grubuna göre anlamlı derecede düşük bulunmuştur. Sonuç: Serum irisin düzeylerinde artma ve kanda visfatin ve vaspin gen ekspresyonunda azalma endometrioziste potansiyel bir biyobelirteç olarak düşünülebilir. Endometriozis varlığında değişiklik gösteren yeni adipositokinlerin ve bu adipositokinler ile inflamasyon arasındaki ilişkinin tanımlanması, endometrioziste rol oynayan mekanizmaların tespitini kolaylaştıracak ve hedeflendirilmiş tedavinin geliştirilmesine yol açacaktır

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SEZGİNER E, KIRLANGIÇ Ö, TANRIVERDİ M, Topçu H, GÜR S (2022). Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis. , 48 - 53. 10.4274/tjps.galenos.2021.52284
Chicago	SEZGİNER Ecem KAYA,KIRLANGIÇ Ömer Faruk,TANRIVERDİ Merve Didem EŞKİN,Topçu Hasan Onur,GÜR Serap Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis. (2022): 48 - 53. 10.4274/tjps.galenos.2021.52284
MLA	SEZGİNER Ecem KAYA,KIRLANGIÇ Ömer Faruk,TANRIVERDİ Merve Didem EŞKİN,Topçu Hasan Onur,GÜR Serap Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis. , 2022, ss.48 - 53. 10.4274/tjps.galenos.2021.52284
AMA	SEZGİNER E,KIRLANGIÇ Ö,TANRIVERDİ M,Topçu H,GÜR S Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis. . 2022; 48 - 53. 10.4274/tjps.galenos.2021.52284
Vancouver	SEZGİNER E,KIRLANGIÇ Ö,TANRIVERDİ M,Topçu H,GÜR S Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis. . 2022; 48 - 53. 10.4274/tjps.galenos.2021.52284
IEEE	SEZGİNER E,KIRLANGIÇ Ö,TANRIVERDİ M,Topçu H,GÜR S "Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis." , ss.48 - 53, 2022. 10.4274/tjps.galenos.2021.52284
ISNAD	SEZGİNER, Ecem KAYA vd. "Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis". (2022), 48-53. https://doi.org/10.4274/tjps.galenos.2021.52284

APA	SEZGİNER E, KIRLANGIÇ Ö, TANRIVERDİ M, Topçu H, GÜR S (2022). Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis. Turkish Journal of Pharmaceutical Sciences, 19(1), 48 - 53. 10.4274/tjps.galenos.2021.52284
Chicago	SEZGİNER Ecem KAYA,KIRLANGIÇ Ömer Faruk,TANRIVERDİ Merve Didem EŞKİN,Topçu Hasan Onur,GÜR Serap Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis. Turkish Journal of Pharmaceutical Sciences 19, no.1 (2022): 48 - 53. 10.4274/tjps.galenos.2021.52284
MLA	SEZGİNER Ecem KAYA,KIRLANGIÇ Ömer Faruk,TANRIVERDİ Merve Didem EŞKİN,Topçu Hasan Onur,GÜR Serap Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis. Turkish Journal of Pharmaceutical Sciences, vol.19, no.1, 2022, ss.48 - 53. 10.4274/tjps.galenos.2021.52284
AMA	SEZGİNER E,KIRLANGIÇ Ö,TANRIVERDİ M,Topçu H,GÜR S Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis. Turkish Journal of Pharmaceutical Sciences. 2022; 19(1): 48 - 53. 10.4274/tjps.galenos.2021.52284
Vancouver	SEZGİNER E,KIRLANGIÇ Ö,TANRIVERDİ M,Topçu H,GÜR S Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis. Turkish Journal of Pharmaceutical Sciences. 2022; 19(1): 48 - 53. 10.4274/tjps.galenos.2021.52284
IEEE	SEZGİNER E,KIRLANGIÇ Ö,TANRIVERDİ M,Topçu H,GÜR S "Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis." Turkish Journal of Pharmaceutical Sciences, 19, ss.48 - 53, 2022. 10.4274/tjps.galenos.2021.52284
ISNAD	SEZGİNER, Ecem KAYA vd. "Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis". Turkish Journal of Pharmaceutical Sciences 19/1 (2022), 48-53. https://doi.org/10.4274/tjps.galenos.2021.52284