Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey

AKIN, Candan; DEMİRCAN, MENEKŞE DİDEM

Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey

MENEKŞE DİDEM DEMİRCAN, (İstanbul Üniversitesi, Su Ürünleri Fakültesi, İstanbul, Türkiye)

Candan AKIN (İstanbul Üniversitesi, Su Ürünleri Fakültesi, İstanbul, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

7 0

Yıl: 2006 Cilt: 30 Sayı: 3 Sayfa Aralığı: 305 - 310 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey

Öz:

Vibriozis hastalığına neden olan etkenlerin başında Vibrio anguiilarum gelir. Bu çalışmada, tipik vibriozis belirtisi gösteren deniz balıklarının iç organlarından 33 Vibrio anguiilarum susu izole edilmiştir ve bu suşiar biyokimyasal testlerle tanımlanmıştır. Sonuçların karşılaştırılması amacıyla, API 20E ve BIONOR Mono-Va aglütinasyon kitleri kullanılmıştır. En son PCR yoluyla suşların rpoN geni çoğaltılmıştır. V. anguiilarum suşlarının fenotipik özellikleri çeşitlilik göstersede, kromozomal DNA' dan rpoN geni tüm suşlardan çoğaltılmıştır. Sonuç olarak bu çalışma, Türkiye'de izole edilen V. anguiiiarum suşlarının tanısında bu genin kullanılabileceğini göstermiştir.

Anahtar Kelime: örnek tanı Listonella anguillarum vibrioz Türkiye Muğla genler deniz balıkları polimeraz zincir reaksiyonu

Konular: Ziraat Mühendisliği

Türkiye'de deniz balıklarında vibriozise neden olan Vibrio anguillarum'un PCR (rpoN geni) ile tanısı

Öz:

The main causative agent of vibriosis is Vibrio anguiilarum. In this study, 33 V. anguiilarum strains were isolated from the internal organs of marine fish showing typical clinical signs of vibriosis, and these strains were identified by biochemical tests. For comparison of these results, API 20E and BIONOR Mono-Va agglutination kits were used. Finally strains were amplified by PCR using the rpoN gene. Although the phenotypic properties of V. anguiilarum strains varied, the rpoN gene from chromosomal DNA was amplified in all strains. In conclusion, this gene could be used in the diagnosis of V. anguiilarum strains isolated in Turkey.

Anahtar Kelime: Listonella anguillarum vibriosis Turkey Mugla genes marine fishes polymerase chain reaction samples identification

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	DEMİRCAN M, AKIN C (2006). Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey. , 305 - 310.
Chicago	DEMİRCAN MENEKŞE DİDEM,AKIN Candan Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey. (2006): 305 - 310.
MLA	DEMİRCAN MENEKŞE DİDEM,AKIN Candan Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey. , 2006, ss.305 - 310.
AMA	DEMİRCAN M,AKIN C Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey. . 2006; 305 - 310.
Vancouver	DEMİRCAN M,AKIN C Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey. . 2006; 305 - 310.
IEEE	DEMİRCAN M,AKIN C "Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey." , ss.305 - 310, 2006.
ISNAD	DEMİRCAN, MENEKŞE DİDEM - AKIN, Candan. "Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey". (2006), 305-310.

APA	DEMİRCAN M, AKIN C (2006). Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey. Turkish Journal of Veterinary and Animal Sciences, 30(3), 305 - 310.
Chicago	DEMİRCAN MENEKŞE DİDEM,AKIN Candan Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey. Turkish Journal of Veterinary and Animal Sciences 30, no.3 (2006): 305 - 310.
MLA	DEMİRCAN MENEKŞE DİDEM,AKIN Candan Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey. Turkish Journal of Veterinary and Animal Sciences, vol.30, no.3, 2006, ss.305 - 310.
AMA	DEMİRCAN M,AKIN C Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey. Turkish Journal of Veterinary and Animal Sciences. 2006; 30(3): 305 - 310.
Vancouver	DEMİRCAN M,AKIN C Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey. Turkish Journal of Veterinary and Animal Sciences. 2006; 30(3): 305 - 310.
IEEE	DEMİRCAN M,AKIN C "Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey." Turkish Journal of Veterinary and Animal Sciences, 30, ss.305 - 310, 2006.
ISNAD	DEMİRCAN, MENEKŞE DİDEM - AKIN, Candan. "Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with vibriosis in marine fish in Turkey". Turkish Journal of Veterinary and Animal Sciences 30/3 (2006), 305-310.