Proje Grubu: TÜBİTAK SBAG Proje Sayfa Sayısı: 75 Proje No: 216S980 Proje Bitiş Tarihi: 15.01.2019 Metin Dili: Türkçe

Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi

Öz:
Proje Başlığı: Rat Oral Mukozasında Ononis Spinosa L.‘nin Yara İyileşmesi Üzerindeki Etkinliğinin Değerlendirilmesi KAPSAM: Oral ülser ağız mukozasının herhangi bir yerine lokalize olan, mukoza epitelinin alt dokularına kadar uzanabilen doku kayıpları ile karakterizedir. Oral ülser, hastaların yaşam kalitesini bozar, ilerlemesi acil servislere başvuru sayısını, destekleyici bakım gereksinimlerini ve hastane masraflarını artırır. Mevcut tedavilerin pahalı olması, yan etkilerinin olması, mikroorganizmaların antimikrobiyal olarak kullanılan ajanlara sürekli direnç kazanması insanları doğal tedaviye yönlendirmektedir. AMAÇ: Bu çalışmada oral ülseri ve oral ülsere bağlı gelişen komplikasyonları azaltarak, hastaların yaşam kalitesini artırmak, tedavi maliyetlerini azaltarak ülke ekonomisine katkı sağlamak amaçlanmıştır. Yara iyileştirici olarak geleneksel tedavide kullanılan, antienflamatuar ve antioksidan özelliklere sahip Ononis spinosa L. bitkisinden elde edilen ekstre ile kullanımı kolay, yan etkisi bulunmayan, bitkisel kökenli ağız bakım solüsyonunun ön çalışmasını yapmak hedeflenmiştir. YÖNTEM: Bu çalışmada Wistar Albino cinsi ratlar kullanıldı. Denekler her grupta 10’ar adet rat olmak üzere 12 gruba ayrıldı. Deneyin 0. Günü anestezi altında grup 1, 2, 3, 4, 5 ve 11’de yer alan ratların sağ ve sol yanak mukozasında 5 mm çaplı, 1 mm derinlikli, standart biyopsi tekniği kullanılarak, punch yardımı ile yara oluşturuldu. 10 gün boyunca, Grup 2 ve Grup 7‘ye Klorheksidin; Grup 11 ve Grup 12’ye CMC-Na ile ağız bakımı uygulandı. Grup 3 ve Grup 8’e %0,5’lik; Grup 4 ve Grup 9‘a %1’lik; Grup 5 ve Grup 10’a ise %1,5’lik Ononis spinosa ekstresi ile ağız bakımı uygulandı. Deney sonucunda tüm gruplardan alınan kan ve doku örnekleriyle makroskopik, biyokimyasal, histopatolojik ve immünohistokimyasal analizler yapılarak yara iyileşmesi değerlendirildi. SONUÇ: Deney süresince ratlarda kilo artışı olduğu belirlendi. Makroskopik evreleme ve yara kapanma miktarına göre de en hızlı yara iyileşmesinin %0,5’lik Ononis spinosa uygulanan yaralı grupta olduğu belirlendi. Makroskopik analizlerle paralel olarak%0,5’lik Ononis spinosa uygulanan yaralı grubun IL-6 ve TNF-α düzeyinin diğer gruplardan anlamlı düzeyde düşük olduğu belirlendi. Makroskopik, biyokimyasal, histopatolojik ve immünohistokimyasal analizler göz önünde bulundurulduğunda en iyi yara iyileşmesinin %0,5’lik Ononis Spinosa dozu uygulanan grupta olduğu belirlendi.
Anahtar Kelime: Klorheksidin Ononis spinosa Rat Oral mukozit Yara iyileşmesi

Konular: Farmakoloji ve Eczacılık
Erişim Türü: Erişime Açık
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APA DEMİR A, SALTAN H, YAZIHAN N, KOCA G, BİLLUR D, ERGENE ÖZ B (2019). Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi. , 1 - 75.
Chicago DEMİR Ayten,SALTAN Hayriye Gülçin,YAZIHAN Nuray,KOCA Gökhan,BİLLUR Deniz,ERGENE ÖZ Burçin Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi. (2019): 1 - 75.
MLA DEMİR Ayten,SALTAN Hayriye Gülçin,YAZIHAN Nuray,KOCA Gökhan,BİLLUR Deniz,ERGENE ÖZ Burçin Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi. , 2019, ss.1 - 75.
AMA DEMİR A,SALTAN H,YAZIHAN N,KOCA G,BİLLUR D,ERGENE ÖZ B Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi. . 2019; 1 - 75.
Vancouver DEMİR A,SALTAN H,YAZIHAN N,KOCA G,BİLLUR D,ERGENE ÖZ B Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi. . 2019; 1 - 75.
IEEE DEMİR A,SALTAN H,YAZIHAN N,KOCA G,BİLLUR D,ERGENE ÖZ B "Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi." , ss.1 - 75, 2019.
ISNAD DEMİR, Ayten vd. "Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi". (2019), 1-75.
APA DEMİR A, SALTAN H, YAZIHAN N, KOCA G, BİLLUR D, ERGENE ÖZ B (2019). Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi. , 1 - 75.
Chicago DEMİR Ayten,SALTAN Hayriye Gülçin,YAZIHAN Nuray,KOCA Gökhan,BİLLUR Deniz,ERGENE ÖZ Burçin Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi. (2019): 1 - 75.
MLA DEMİR Ayten,SALTAN Hayriye Gülçin,YAZIHAN Nuray,KOCA Gökhan,BİLLUR Deniz,ERGENE ÖZ Burçin Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi. , 2019, ss.1 - 75.
AMA DEMİR A,SALTAN H,YAZIHAN N,KOCA G,BİLLUR D,ERGENE ÖZ B Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi. . 2019; 1 - 75.
Vancouver DEMİR A,SALTAN H,YAZIHAN N,KOCA G,BİLLUR D,ERGENE ÖZ B Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi. . 2019; 1 - 75.
IEEE DEMİR A,SALTAN H,YAZIHAN N,KOCA G,BİLLUR D,ERGENE ÖZ B "Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi." , ss.1 - 75, 2019.
ISNAD DEMİR, Ayten vd. "Rat Oral Mukozasında Ononis Spinosa L.`Nin Yara İyileşmesi Üzerindeki Etkinliğinin Belirlenmesi". (2019), 1-75.