Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus

Yarim, Gul Fatma; ÇİFTCİ, GÜLAY; ÇENESİZ, Sena; Ciftci, Alper; NİSBET, Cevat

Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus

Cevat NİSBET, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Samsun, Türkiye)

Gul Fatma Yarim, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Samsun, Türkiye)

ALPER ÇİFTCİ, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Kontrol Bölümü, Samsun,Türkiye)

Sena ÇENESİZ, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Samsun, Türkiye)

Gülay ÇİFTCİ (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Samsun, Türkiye)

Ankara Üniversitesi Veteriner Fakültesi Dergisi

5 0

Yıl: 2007 Cilt: 54 Sayı: 3 Sayfa Aralığı: 159 - 163 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus

Öz:

Bu çalışmanın amacı Brucella abortus (B. abortus) ile enfekte sığırlarda serum nitrik oksit (NO) ve malondialdehit (MDA) düzeylerini belirlemektir. Çalışmanın materyalini 20 adet B. abortus ile enfekte ve 10 adet sağlıklı olmak üzere 2-3 yaşlarında 30 adet sığır oluşturdu. B. abortus infeksiyonunun tespiti Serum Aglutinasyon Testi ve Multipleks PCR ile gerçekleştirildi. Serum NO düzeyi enzimatik Greiss reaksiyonu ile MDA düzeyi Yoshioka metodu ile belirlendi. B. abortus ile enfekte sığırlarda serum NO ve MDA düzeyleri sırası ile 58.20 ± 6.50 μmol/L ve 2.25 ± 0.10 nmol/ml, kontrol sığırlarda ise 38.07 ± 2.40 μmol/L ve 1.74 ± 0.25 nmol/ml bulundu. Enfekte sığırlarda serum NO ve MDA düzeylerinin sağlıklı kontrollerden istatistik olarak önemli oranda yüksek olduğu belirlendi (sırası ile, p < 0.05 ve p < 0.01). Sonuç olarak, NO düzeyindeki artışın bakteriyel lipopolisakkaridler ile makrofajlardan NO sentezinin uyarılmasının ve MDA düzeyindeki artışın da brusellozis enfeksiyonunun membran lipidlerini etkileyerek aşırı radikal üretimine neden olmasının sonucu olabileceği ve doku hasarının bir göstergesi olarak kullanılabileceği kanaatine varıldı.

Anahtar Kelime: brusellozis Brucella sığır malondialdehit Brucella abortus nitrik oksit tanı teknikleri

Konular: Veterinerlik

Brucella abortus ile enfekte sığırlarda serum nitrik oksit ve malondialdehit düzeylerinin araştırılması

Öz:

The objective of this study was to determine serum nitric oxide (NO) and malondialdehyde (MDA) levels in cattle infected with Brucella abortus (B. abortus). Study cohort was comprised of 30 cattle aged between 2-3 years; 20 infected with B. abortus and 10 healthy cattle. B. abortus infection was determined in the animals by Serum Agglutination Test and Multiplex PCR assay. Serum NO level was determined by enzymatic Greiss reaction and MDA level by Yoshioka method. Mean serum NO and MDA levels of cases with confirmed B. abortus were 58.20 ± 6.50 μmol/L and 2.25 ± 0.10 nmol/ml, respectively whereas those of the controls were 38.07 ± 2.40 μmol/L and 1.74 ± 0.25 nmol/ml, respectively. We found that serum NO and MDA levels in infected cattle were significantly higher than healthy control cattle (p < 0.05 and p < 0.01, respectively). In conclusion, the increase in NO level in cattle infected with B. abortus is a result of stimulation of NO synthesis in macrophages by bacterial lipopolysaccharides, and the increase in MDA level is a result of excessive radical production due to brucellosis acting upon membrane lipids and can be used as an indicator of tissue damage.

Anahtar Kelime: nitric oxide diagnostic techniques brucellosis Brucella cattle malondialdehyde Brucella abortus

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	NİSBET C, Yarim G, Ciftci A, ÇENESİZ S, ÇİFTCİ G (2007). Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus. , 159 - 163.
Chicago	NİSBET Cevat,Yarim Gul Fatma,Ciftci Alper,ÇENESİZ Sena,ÇİFTCİ GÜLAY Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus. (2007): 159 - 163.
MLA	NİSBET Cevat,Yarim Gul Fatma,Ciftci Alper,ÇENESİZ Sena,ÇİFTCİ GÜLAY Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus. , 2007, ss.159 - 163.
AMA	NİSBET C,Yarim G,Ciftci A,ÇENESİZ S,ÇİFTCİ G Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus. . 2007; 159 - 163.
Vancouver	NİSBET C,Yarim G,Ciftci A,ÇENESİZ S,ÇİFTCİ G Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus. . 2007; 159 - 163.
IEEE	NİSBET C,Yarim G,Ciftci A,ÇENESİZ S,ÇİFTCİ G "Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus." , ss.159 - 163, 2007.
ISNAD	NİSBET, Cevat vd. "Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus". (2007), 159-163.

APA	NİSBET C, Yarim G, Ciftci A, ÇENESİZ S, ÇİFTCİ G (2007). Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 54(3), 159 - 163.
Chicago	NİSBET Cevat,Yarim Gul Fatma,Ciftci Alper,ÇENESİZ Sena,ÇİFTCİ GÜLAY Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus. Ankara Üniversitesi Veteriner Fakültesi Dergisi 54, no.3 (2007): 159 - 163.
MLA	NİSBET Cevat,Yarim Gul Fatma,Ciftci Alper,ÇENESİZ Sena,ÇİFTCİ GÜLAY Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.54, no.3, 2007, ss.159 - 163.
AMA	NİSBET C,Yarim G,Ciftci A,ÇENESİZ S,ÇİFTCİ G Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2007; 54(3): 159 - 163.
Vancouver	NİSBET C,Yarim G,Ciftci A,ÇENESİZ S,ÇİFTCİ G Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2007; 54(3): 159 - 163.
IEEE	NİSBET C,Yarim G,Ciftci A,ÇENESİZ S,ÇİFTCİ G "Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 54, ss.159 - 163, 2007.
ISNAD	NİSBET, Cevat vd. "Investigation of serum nitric oxide and malondialdehyde levels in cattle infeted with Brucella abortus". Ankara Üniversitesi Veteriner Fakültesi Dergisi 54/3 (2007), 159-163.