Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus

SARER, Engin; Gökbulut, Alper

Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus

Engin ŞARER, (Ankara Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, Ankara, Türkiye)

Alper GÖKBULUT (Ankara Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, Ankara, Türkiye)

Turkish Journal of Pharmaceutical Sciences

1 0

Yıl: 2008 Cilt: 5 Sayı: 3 Sayfa Aralığı: 167 - 174 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus

Öz:

Türkiye’nin üç farklı yöresinden toplanan Vitex agnus castus bitkisinin yaprak ve meyvalarmdakikafeik ve klorojenik asit tayini yüksek basıngh sıvı kromatografisi yöntemi uygulanarakgergekleştirilmiştir. Kafeik ve klorojenik asit potansiyel antioksidanlar olarak bilinmektedir ve Türkiyedeyeti§en Vitex agnus castus örneklerinde bu iki fenolik asit ilk defa incelenmiştir. Bitkiden elde edilen altıekstrenin kalitatif ve kantitatif analizleri RP-HPLC yöntemi He yapılmi§tır. Kafeik asit ve klorojenik asitigin sırasıyla 1.02-400 (r 20.9999) ve 1.08-476 (r 20.9948) /ug/mL konsantrasyon arahklarında mükemmeldoğrusalhk elde edilmiştir. Marmaris ve Antalya yaprak ekstrelerinde kafeik asit miktarlan en yüksekseviyede olup sırasıyla %0.277(a/a) ve %0.266(a/a) oramnda iken Marmaris meyva ve Isparta meyvaekstreleri klorojenik asit agisından zengindir ve sırasıyla % 0.343(a/a) ve %0.303(a/a) oramndaklorojenik asit igermektedir.

Anahtar Kelime: Kromatografi, yüksek basınçlı sıvı Vitex fenolik asit Nitel araştırma Konu ile ilgili değerlendirme çalışmaları Kafeik asitler

Konular: Farmakoloji ve Eczacılık

Vitex agnus castus yaprak ve meyvalarında kafeik asit ve klorojenik asit tayini

Öz:

A high performance liquid chromatographic method was applied to the determination of caffeic andchlorogenic acids in the leaves and fruits of Vitex agnus-castus collected from three different region ofTurkey. Caffeic and chlorogenic acids were known to be potent antioxidants and the two phenolic acidswere investigated in Turkish Vitex agnus-castus samples for the first time. The qualitative andquantitative analysis of six extracts of the plant were performed by RP-HPLC method. The linear rangeof detection for caffeic and chlorogenic acids were between 1.02-400 (r 20.9999) and 1.08-476 (r 20.9948) jig/mL, respectively. Marmaris and Antalya leaf samples display the highest content of caffeicacid as 0.277%(w/w) and 0.266%(w/w) while Marmaris fruit and Isparta fruit samples were rich inchlorogenic acid as 0.343%(w/w) and 0.303%(w/w), respectively.

Anahtar Kelime: Caffeic Acids Chromatography, High Pressure Liquid Vitex phenolic acid Qualitative Research Evaluation Studies as Topic

Konular: Farmakoloji ve Eczacılık

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SARER E, Gökbulut A (2008). Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus. , 167 - 174.
Chicago	SARER Engin,Gökbulut Alper Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus. (2008): 167 - 174.
MLA	SARER Engin,Gökbulut Alper Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus. , 2008, ss.167 - 174.
AMA	SARER E,Gökbulut A Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus. . 2008; 167 - 174.
Vancouver	SARER E,Gökbulut A Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus. . 2008; 167 - 174.
IEEE	SARER E,Gökbulut A "Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus." , ss.167 - 174, 2008.
ISNAD	SARER, Engin - Gökbulut, Alper. "Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus". (2008), 167-174.

APA	SARER E, Gökbulut A (2008). Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus. Turkish Journal of Pharmaceutical Sciences, 5(3), 167 - 174.
Chicago	SARER Engin,Gökbulut Alper Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus. Turkish Journal of Pharmaceutical Sciences 5, no.3 (2008): 167 - 174.
MLA	SARER Engin,Gökbulut Alper Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus. Turkish Journal of Pharmaceutical Sciences, vol.5, no.3, 2008, ss.167 - 174.
AMA	SARER E,Gökbulut A Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus. Turkish Journal of Pharmaceutical Sciences. 2008; 5(3): 167 - 174.
Vancouver	SARER E,Gökbulut A Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus. Turkish Journal of Pharmaceutical Sciences. 2008; 5(3): 167 - 174.
IEEE	SARER E,Gökbulut A "Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus." Turkish Journal of Pharmaceutical Sciences, 5, ss.167 - 174, 2008.
ISNAD	SARER, Engin - Gökbulut, Alper. "Determination of caffeic and chlorogenic acids in the leaves and fruits of vitex agnus-castus". Turkish Journal of Pharmaceutical Sciences 5/3 (2008), 167-174.