Yıl: 2009 Cilt: 6 Sayı: 3 Sayfa Aralığı: 163 - 176 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations

Öz:
Bu çalışmada asemetasin 'in farmasötik preparatlarda miktar tayini için üç yeni spektrofotometrik yöntem, orijinal UV spektrofotometri, birinci Ve ikinci türev spektrofotometri, geliştirilmiştir. Orijinal UVspektrofotometride absorbans değerleri, asemetasin'in 0,1 NNaOH içerisindeki çözeltilerinin 210-325 nm aralığındaki UV spektrumlarında 280.0 nm de ölçülmüştür. Birinci türev UV spektrofotometride, dA/dA değerleri, asemetasin in 0,1 N NaOH içerisindeki çözeltilerinin 230-325 nm aralığındaki birinci türev UV spektrumlarında (AX= 2 nm) 240.0 nm de ölçülmüştür. İkinci türev UV spektrofotometride d2A/d A değerleri asemetasin'in 0,1 N NaOH içerisindeki çözeltilerinin 240-325 nm aralığındaki ikinci türev UV spektrumlarında (AX— 2 nm) 274.0 nm de ölçülmüştür. Çalışmada doğrusal çalışma aralığı orijinal UV spektrofotometri ve birinci türev spektrofotometri için 8.0 — 85.0 jug/mL, ikinci türev spektrofotometri için ise 15.0 — 85.0 /jg/mL olarak bulunmuştur. Yöntemlerdeki ortalama geri kazanım ve bağıl standart sapma değerleri sırasıyla orijinal UV spektrofotometrik yöntemde % 100.64 ve % 0.95, birinci türev UV spektrofotometrik yöntemde % 100.36 ve % 0.62 ve, ikinci türev UV spektrofotometrik yöntemde % 100.45 ve % 1.10 olarak bulunmuştur. Ayrıca tarafımızdan yeni bir YPSK yöntemi geliştirilmiştir. Bu yöntemde, optimal kromatografik ayırım, ACE C18 kolonu ve lmL/dk akış hızında asetonitril-su (80:20, v/v) mobil fazı ve 280 nm 'de deteksiyonla sağlanmıştır. İç standart olarak dienogest kullanılmıştır. Geliştirilen yöntemler Türkiye ilaç piyasasında bulunan iki adet tablet formulasyonuna başarıyla uygulanmıştır. Elde edilen tüm sonuçlar kendi aralarında istatistiksel olarak karşılaştırılmıştır.
Anahtar Kelime: Kromatografi, yüksek basınçlı sıvı Spektrofotometri Kromatografi, sıvı asemetasin Farmasötik preparasyonlar

Konular: Farmakoloji ve Eczacılık

Asemitasin'in farmasötik preparatlarda spektrofotometrik ve sıvı kromatografik yöntemlerle miktar tayini

Öz:
In present study, three new spectrophotometric methods, original UV spectrophotometry, first and second order derivative UV spectrophotometry and a new liquid chromatographic method were developed for the determination of acemetacin in pharmaceutical preparations. In original UV spectrophotometry, absorbances were measured at 280,0 nm in the zero order UV spectra of the solution of acemetacin in O,1N NaOH in the range of 210 - 325 nm. In first derivative UV spectrophotometry, dA/dX values were measured at 240.0 nm in the first derivative UV spectra of the solution of acemetacin in 0,1 N NaOH in the range of 230 - 325 nm (AX= 2 nm). In second derivative UV spectrophotometry d2A/dA2 values were measured at 274.0 nm in the second derivative UV spectra of the solution of acemetacin in 0,1 NNaOH in the range of 240 - 325 nm (AX= 2 nm). Linearity range was found as 8.0 - 85.0 jUg/mL in original UV spectrophotometric and first order derivative UV spectrophotometric methods and 15.0 — 85.0 jUg/mL in second order derivative UV spectrophotometric method. Mean recoveries and the relative standard deviations of the methods were found as 100.64 % and 0.95 % in original UV spectrophotometic method 100.36 % and 0.62 % in first derivative UV spectrophotometric method and, 100.45 % and 1.10 % in second derivative UV spectrophotometric method respectively. Also, a new HPLC method was developed. In this method, ACE C18 analytical column and a mobile phase composed of acetonitrile - water (80:20, v/v) at a flow rate of 1.0 mL/min was used for the optimal chromatographic separation using UV detection at 280 nm. Dienogest was used as internal standard. All the methods developed were successfully applied to two tablet formulations commercially available in Turkish drug market. All the results were compared statistically with each other.
Anahtar Kelime: Chromatography, High Pressure Liquid Spectrophotometry Chromatography, Liquid acemetacin Pharmaceutical Preparations

Konular: Farmakoloji ve Eczacılık
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
0
0
0
  • 1.Castro, B., Gameiro, P., Lima, J. L.F.C., Matos, C, Reis, S."Interaction of drugs with hexadecylphosphocoline micelles. Derivative spectroscopy, acid-base and solubility" Materials Sciences and Engineering, C, 18,71-78, 2001.
  • 2.Arcos, M.J., Ortiz, M.C., Villahoz, B., Sarabia, L. A.,"Genetic algorithm-based wavelenghts selection in multicomponent spectrometric determinations by PLS:application on indomethacin and acemethacin mixtıro"Anafytica Chimica Açta, 339, 63-77, 1997. 3.
  • Gilabert, L.E., Biosca, Y.M., Sagrado, S.,Camanas, R.M.V., "Quality control of Pharmaceuticals containing non-steroidal anti-inflammatory drugs by micellar liquid chromatography" Chromatographia, 55(5-6), 283-285, 2002.
  • 4. Martinez-Algaba, C, Escuder-Gilabert, L., Sagrado, S., Villanueva-Camanas, R.M., Medina-Hernandez, M.J."Comparison between sodium dodecylsulphate and cetyltrimethylammonium bromide as mobile phases in the micellar liquid chromatography determination of non-steroidal anti-inflammatory drugs in pharmaceuticals" J.Pharm. Biomed.Anal.,36, 393-399, 2004.
  • 5.Kanberoğlu, G.S. "Antiinflamatuvar-analjezik ilaçlardan asemetasin'in akış enjeksiyon analiz yöntemi ile miktar tayini", Yüksek lisans tezi, Van , 2004.
  • 6.Schöllnhammer, G., Dell H.D., Doersing, K., Kamp, R. "Quantitative determination of acemetacin and its metabolite indometacin blood and plasma by column liquid chromatography",/.C7zromafogr.,375, 331-338,1986.
  • 7.Notarianni, L."Method for the determination of acemetacin, a non-steroidal anti-inflammatory drug, in plasma by high performance liquid chromatography"/.C/*romafogr.,413, 305-308,1987.
  • 8.Pang, J. and Hengshan, TV'HPLC determination of acemetacin and its metabolite indometacin in human plasma" Zhongguo Yiyuan Yaoxue Zazhi, 24(4), 246-247, 2004.
  • 9.Zouvelekis, D., Yannakopoulou, K., Mavridis, I. M.,"The self-association of the drug acemetacin and its interactions and stabilization with |3-cyclodextrin in aqueous solution as inferre from NMR spectroscopy and HPLC studies"Carbonhydrate Research, 337, 1387-1395,2002.
  • 10.Hu, Y.,Liu, H., Ma, R., Wang, J., Hou, Y., "Determination of acemetacin and indometacin in human serum by high performance liquid chromatography" Sepu, 17(6), 586-587, 1999.
  • 11. Shi, X., Chen, M., Zhang, J., Zhang, L., Wang, H., "Determination of acemetacin and its metabolite indometacin in human plasma by solid phase extraction HPLC with UV detection" Zhongguo Yiyao Zazhi, 32(10), 454-456, 2001.
  • 12.Eunmi, B., Jung, C, Dong, J., Xiang, P., Jin, K., Jun, J., Chong, K., "HPLC method for the pharmacokinetics study of acemetacin in human plasma" J. Liq. Chromatogr.Rel.Tech., 28,1593-1604,2005.
  • 13.Li, D., Lu, W., W, X., Wang, J., Zhang, H., Zhang, R., Wang, G., Zang, X., Zang, Q., "Pharmacokinetics of indomethacin, a metabolite of acemetacin following a single dose and multiple doses administrated as acemetacin sustained-release tablets in healthy male volunteers" Journal ofHelath Science, 31(3), 308-310, 2005.
  • 14.Reguera, C, Cruz, O. M., Arcos, J.M."Differential pulse voltammetric simultaneous determination of four anti-inflammatory drugs by using soft mo&QYmg'Electroanalysis, 14(24), 1699-1706,2002.
  • 15.Reguera, C, Arcos, J.M., Cruz, O. M. "An optimization procedure for determination of indometacin and acemetacin by differential pulse adsorptive stripping voltammetry. Application on urine samples "Talanta, 46, 1493-1505,1998.
APA ÖZBAKAN H, PALABIYIK İ, ÇAĞLAYAN M, ONUR F (2009). Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations. , 163 - 176.
Chicago ÖZBAKAN Hakan,PALABIYIK İ. Murat,ÇAĞLAYAN M. Gökhan,ONUR Feyyaz Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations. (2009): 163 - 176.
MLA ÖZBAKAN Hakan,PALABIYIK İ. Murat,ÇAĞLAYAN M. Gökhan,ONUR Feyyaz Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations. , 2009, ss.163 - 176.
AMA ÖZBAKAN H,PALABIYIK İ,ÇAĞLAYAN M,ONUR F Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations. . 2009; 163 - 176.
Vancouver ÖZBAKAN H,PALABIYIK İ,ÇAĞLAYAN M,ONUR F Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations. . 2009; 163 - 176.
IEEE ÖZBAKAN H,PALABIYIK İ,ÇAĞLAYAN M,ONUR F "Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations." , ss.163 - 176, 2009.
ISNAD ÖZBAKAN, Hakan vd. "Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations". (2009), 163-176.
APA ÖZBAKAN H, PALABIYIK İ, ÇAĞLAYAN M, ONUR F (2009). Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations. Turkish Journal of Pharmaceutical Sciences, 6(3), 163 - 176.
Chicago ÖZBAKAN Hakan,PALABIYIK İ. Murat,ÇAĞLAYAN M. Gökhan,ONUR Feyyaz Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations. Turkish Journal of Pharmaceutical Sciences 6, no.3 (2009): 163 - 176.
MLA ÖZBAKAN Hakan,PALABIYIK İ. Murat,ÇAĞLAYAN M. Gökhan,ONUR Feyyaz Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations. Turkish Journal of Pharmaceutical Sciences, vol.6, no.3, 2009, ss.163 - 176.
AMA ÖZBAKAN H,PALABIYIK İ,ÇAĞLAYAN M,ONUR F Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations. Turkish Journal of Pharmaceutical Sciences. 2009; 6(3): 163 - 176.
Vancouver ÖZBAKAN H,PALABIYIK İ,ÇAĞLAYAN M,ONUR F Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations. Turkish Journal of Pharmaceutical Sciences. 2009; 6(3): 163 - 176.
IEEE ÖZBAKAN H,PALABIYIK İ,ÇAĞLAYAN M,ONUR F "Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations." Turkish Journal of Pharmaceutical Sciences, 6, ss.163 - 176, 2009.
ISNAD ÖZBAKAN, Hakan vd. "Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations". Turkish Journal of Pharmaceutical Sciences 6/3 (2009), 163-176.