Yıl: 2022 Cilt: 7 Sayı: 4 Sayfa Aralığı: 537 - 545 Metin Dili: İngilizce DOI: 10.35229/jaes.1180420 İndeks Tarihi: 06-01-2023

Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?

Öz:
Abstract: The aim of this study is to determine the effect of growing media on antioxidant properties and antimicrobial activities of Pleurotus ostreatus(Jacq. ex Fr.) P. Kumm. In the study, growing substrates were prepared with five tree sawdust (80%) and waste (20% Tea Waste or Wheat Bran) at different ratios (80% Sawdust + 20% TW, 80% Sawdust + 20% GW). The antimicrobial activities of methanol extracts from 19 P. ostreatus against microorganisms were tested by agar well diffusion technique. The total phenolic content was measured using the Folin-Ciocalteau procedure. The antioxidant capacity was determined using ferric reducing antioxidant power (FRAP) and free radical scavenging activity of DPPH. It was observed that the addition of 20% tea waste in the substrate had a positive effect on antimicrobial activity. It was determined that the methanol extract of mushrooms obtained from the 80QS+20TW substrate was the most effective extract against to all of microorganisms investigated in this study. Significant differences (P
Anahtar Kelime: Antimicrobial Antioxidant Mushroom Pleurotus ostreatus Total phenolic.

Farklı substratlar, Pleurotus ostreatus'un antioksidan özelliklerini ve antimikrobiyal aktivitesini etkiler mi?

Öz:
Öz: Bu çalışmanın amacı, yetiştirme ortamının Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm.'un antioksidan özellikleri ve antimikrobiyal aktiviteleri üzerindeki etkisini belirlemektir. Çalışmada beş adet talaş (%80) ve atık (%20 Çay Atığı veya Buğday Kepeği) ile farklı oranlarda (%80 Talaş + %20 TW, %80 Talaş + 20% GW) yetiştirme substratları hazırlanmıştır. Toplam 19 farklı ortamda yetiştirilen P. ostreatus'tan elde edilen metanol ekstraktların, mikroorganizmalara karşı antimikrobiyal aktiviteleri agar kuyu difüzyon tekniği ile test edildi. Toplam fenolik içerik, Folin-Ciocalteau prosedürü kullanılarak ölçülmüştür. Antioksidan kapasitesi, DPPH'nin ferrik indirgeyici antioksidan gücü (FRAP) ve serbest radikal süpürücü aktivitesi kullanılarak belirlendi.. 80QS+20TW substratından elde edilen mantarların metanol ekstraktının bu çalışmada incelenen tüm mikrooganizmalara karşı en etkili ekstrakt olduğu belirlendi. Farklı substratlar üzerinde yetiştirilen mantar ekstraktlarının antioksidan içerikleri arasında önemli farklılıklar (P
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Akindahunsi, A.A. & Oyetayo, F.L. (2006). Nutrient and antinutrient distribution of edible mushroom, Pleurotus tuber-regium (Fries). LWT-Food Science and Technology, 39, 548-53. DOI: 10.1016/j.lwt .2005.04.005
  • Akyüz, M. & Kırbağ, S. (2010). Nutritive value of wild edible and cultured mushrooms. Turkish Journal Biology, 34, 97-102. DOI: 10.3906/biy-0805-17
  • Alves, M., Ferreira, I., Martins, A. & Pintado, M. (2012a). Antimicrobial activity of wild mushroom extracts against clinical isolates resistant to different antibiotics, Journal of Applied Microbiology 113(2), 466-475. DOI: 10.1111/j.1365-2672.2012.05347.x
  • Arbaayah, H.H. & UmiKalsom, Y. (2013). Antioxidant properties in the oyster mushrooms (Pleurotus spp.) and splitgill mushroom (Schizophyllum commune) ethanolic extracts. Mycosphere, 4(4), 661-673. DOI: 10.5943/mycosphere/4/4/2
  • Archana, S. & Abraham, J. (2011). Comparative analysis of antimicrobial activity of leaf extracts from fresh green tea, commercial green tea and black tea on pathogens. Journal of Applied Pharmaceutical Science, 01(08), 149-152.
  • Bakir, T., Karadeniz, M. & Unal, S. (2018). Investigation of antioxidant activities of Pleurotus ostreatus stored at different temperatures. Food science & nutrition, 6(4), 1040-1044. DOI: 10.1002/fsn3.644
  • Baysal, E., Peker, H., Yalınkılıç, M.K. & Temiz, A. (2003). Cultivation of oyster mushroom on waste paper with some added supplementary materials. Bioresource Technology, 89(1), 95-97. DOI: 10.1016/s0960-8524(03)00028-2
  • Benzie, I.F.F. & Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay. Analytical Biochemistry, 239, 70-76. DOI: 10.1006/abio.1996.0292
  • Benzie, I.F.F. & Szeto, Y.T. (1999). Total antioxidant capacity of teas by the ferric reducing/antioxidant power assay. Journal Agricultural and Food Chemistry, 47, 633-636. DOI: 10.1021/jf9807768
  • Bozdogan, A., Ulukanlı, Z., Bozok, F., Eker, T., Doğan, H.H. & Büyükalaca, S. (2018). Antioxidant Potential of Lactarius deliciosus and Pleurotus ostreatus from Amanos Mountains. Advancements in Life Sciences, 5(3), 113-120.
  • Centko, R.M., Ramon-Garcia, S., Taylor, T., Patrick, B.O., Thompson, C.J., Miao, V.P. & Andersen, R.J. (2012). Ramariolides A-D, antimycobacterial butenolides isolated from the mushroom Ramaria cystidiophora. Journal Of Natural Products, 75(12), 2178-82. DOI: 10.1021/np3006277
  • Chiavaroli, V., Giannini, C, De Marco, S., Chiarelli, F. & Mohn, A. (2011). Unbalanced oxidant- antioxidant status and its effects in pediatric diseases. Redox Report, 16, 101-107. DOI: 10.1179/174329211X13049
  • Cuendet, M., Hostettmann, K. & Potterat, O. (1997). Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta, 80, 1144-1152. DOI: 10.1002/hlca.19970800411.
  • Demirhan, A., Yeşil, Ö.F., Yıldız, A. & Gül, K. (2007). Bazı makrofungus türlerinin antimikrobiyal aktiviteleri üzerine bir araştırma. Fırat Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 19(4), 425- 433.
  • Doğan, N. & Cemhan, D. (2021). Determination of antioxidant activity of oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm.) according to different flush times and the ınhibition potential of α -amylase and αglucosidase, which are key enzyme role in hyperglycemia. KSU J. Agric Nat 24(6), 1177-1186. DOI: 10.18016/ksutarimdoga.vi.853006
  • Earnshaw, D.M., Dlamini, B. & Masarirambi, M.T. (2012). Growth and yield of oyster mushroom (Pleurotus ostreatus) grown on different substrates ammended with varying levels of wheat bran. International Journal of Life Sciences, 1(4), 111- 117.
  • Elmastas, M., Isıldak, O., Türkekul, I. & Temura, N. (2007). Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms, Journal of Food Composition and Analysis, 20, 337-345. DOI: 10.1016/j.jfca.2006.07.003
  • Eren, E. & Pekşen, A. (2014). Türkiye’de kültür mantarı üretimi, sorunları ve çözüm yolları. 1.Ulusal Mikoloji Günleri, Erzurum, 01-04 Eylül 2014.
  • Farhoosh, R., Golmovahhed, G. & Khodaparast, M. (2007). Antioxidant activity of various extracts of old tea leaves and black tea wastes (Camellia sinensis L.). Food Chemistry, 100, 231-236. DOI: 10.1016/j.foodchem.2005.09.046
  • García-Lafuente, A., Moro, C., Villares, A., Guillamón, E., Rostagno, M.A., DArrigo, M. & Martínez, J.A. (2011). Mushrooms as a source of anti- inflammatory agents. American Journal of Community Psychology, 48(1-2), 125-141. DOI: 10.2174/187152310791110643
  • Gashaw, G., Fassil, A. & Redi, F. (2020). Evaluation of the antibacterial activity of Pleurotus spp. cultivated on different agricultural wastes in Chiro, Ethiopia, International Journal of Microbiology, ID 9312489, 1-9, DOI: 10.1155/2020/9312489
  • Güçlü, Ü.Ö., Erşan, S., Özcan, E., Özan, G., Kayra, N. & Ekinci, F.Y. (2016). Black tea processing waste as a source of antioxidant and antimicrobial phenolic compounds. European Food Research & Technology, 242, 1523-1532 DOI: 10.1007/s00217-016-2653-9
  • Gülser, C. & Pekşen, A. (2003). Using tea waste as a new casing material in mushroom (Agaricus bisporus (L.)Sing.) cultivation. Bioresource Technology, 88, 153-156. DOI: 10.1016/S0960- 8524(02)00279-1
  • Harikrishnan, R., Balasundaram, C. & Heo, M.S. (2011). Diet enriched with mushroom Phellinus linteus extract enhances the growth, innate immune response, and disease resistance of kelp grouper, Epinephelus bruneus against vibriosis, Fish & Shellfish Immunology, 30(1), 128-134. DOI: 10.1016/j.fsi.2010.09.013
  • Harikrishnan, R., Balasundaram, C. & Heo, M.S. (2012). Effect of Inonotus obliquus enriched diet on hematology, immune response, and disease protection in kelp grouper, Epinephelus bruneus against Vibrio harveyi. Aquaculture, 344-349, 48- 53. DOI: 10.1016/j.aquaculture.2012.03.010
  • Hearst, R., Nelson, D., McCollum, G., Cherie, Millar, B., Yasunori M., Goldsmith, C.E., Rooney, P.J., Loughrey, A., Rao J.R. & Moore, J.E. (2009). An examination of antibacterial and antifungal properties of constituents of Shiitake (Lentinula edodes) and oyster (Pleurotus ostreatus) mushrooms. Complementary Therapies in Clinical Practice, 15(1), 5-7, DOI: 10.1016/j.ctcp.2008.10.002
  • Kacar, B. (1987). Çayın biyokimyasl ve işlenme teknolojisi. Çay işletmeleri Genel Müdürlüğü, Yayını No. 6, DSİ Matbaası, Ankara, 329s.
  • Kalac, P. (2009). Chemical composition and nutritional value of European species of wild growing mushrooms: A review. Food Chemistry, 11(1), 9- 16. DOI: 10.1016/j.foodchem.2008.07.077
  • Kalac, P. & Svoboda, L. (2001). A review of trace element concentrations in edible mushrooms. Food Chemistry, 69, 273-281. DOI: 10.1016/S0308- 8146(99)00264-2
  • Kumari, B. & Atri, N.S. (2014). Nutritional and nutraceutical potential of wild edible macrolepiotoid mushrooms of north India. International Journal of Pharmacy and Pharmaceutical Sciences, 6(2), 200-204.
  • Kumla, J., Suwannarach, N., Sujarit, K., Penkhrue, W., Kakumyan, P., Jatuwong, K., Vadthanarat, S. & Lumyong, S. (2020). Cultivation of mushrooms and their lignocellulolytic enzyme production through the utilization of agro-ındustrial waste. Molecules (Basel, Switzerland), 25(12), 2811. DOI: 10.3390/molecules25122811
  • Mau, J.L., Lin, H.C. & Chen, C.C. (2002). Antioxidant properties of several medicinal mushrooms. Journal Agricultural and Food Chemistry, 50, 6072-6077. DOI: 10.1021/jf0201273
  • Menaga, D., Rajakumar, S. & Ayyasamy, P.M. (2013). Free radical scavenging activity of methanolic extract of Pleurotus florida mushroom. International Journal of Pharmacy and Pharmaceutical Sciences, 5(4), 601-606.
  • Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal Science Technology, 26(2), 211-219.
  • Mossebo, D.C., Metsebing, B.P., Oba, R., Tsigaing Tsigaing, F., Ryvarden, L., Fonkui, T.Y., Mungoh Tata, C. & Ndinteh, D.T. (2020). Comparative evaluation of antifungal and antibacterial activities of crude extracts of Pleurotus sajor-caju, Pleurotus tuber-regium and Lentinus squarrosulus (Basidiomycota, Pleurotaceae, Lentinaceae) from Cameroon. European Journal of Biology and Biotechnology, 1(5). DOI: 10.24018/ejbio.2020.1.5.97
  • Nichenametla, S.N., Taruscio, T.G., Barney, D.L. & Exon, J.H. (2006). A review of the effects and mechanisms of polyphenolics in cancer. Critical Reviews in Food Science and Nutrition, 46, 161- 183. DOI: 10.1080/10408390591000541
  • Okwulehie, I.C. & Ogoke, J.A. (2013). Bioactive, nutritional and heavy metal constituents of some edible mushrooms found in Abia State of Nigeria. International Journal of Applied Microbiology and Biotechnology Research (IJAMBR), 1, 7-15. DOI: 10.33500/ijambr.2013.01.002
  • Oyetayo, V.O. & Ariyo, O.O. (2013). Antimicrobial and antioxidant properties of Pleurotus ostreatus (Jacq: Fries) cultivated on different tropical woody substrates. Journal of Waste Conversion Bioproducts and Biotechnology, 1(2), 28-32. DOI: 10.5147/jwcbb.2013.0121
  • Pala, S.A. & Wani, A.H. (2011). Antioxidant activity of some wild mushrooms of Kashmir Valley. Bioresearch Bulletin, 2, 125-129.
  • Pekşen, A. & Yakupoğlu, G. (2009). Tea waste as a supplement for the cultivation of Ganoderma lucidum. World Journal of Microbiology and Biotechnology, 25, 611-618. DOI: 10.1007/s11274-008-9931-z
  • Perez, C., Pauli, M. & Bazerque, P. (1990). An antibiotic assay by the well agar method. Acta Biologia et Medicine Experimentalis, 15, 113-115.
  • Ponmurugan, P., Sekhar, Y.N. & Sreeshakti, T.R. (2007). Effect of various substrates on the growth and quality of mushrooms. Pakistan Journal of Biological Sciences, 10, 171-173. DOI: 10.3923/pjbs.2007.171.173
  • Porgal, E. & Büyüktuncel, E. (2012). Determination of phenolic composition and antioxidant capacity of native red wines by high performance liquid chromatography and spectrophotometric methods. Food Research International, 45, 145-154. DOI: 10.1016/j.foodres.2011.10.025
  • Ruiz-Rodriguez, A., Soler-Rivas, C., Polonia, I. & Wichers, J.H. (2010). Effect of olive mill waste (OMW) supplementation to Oyster mushrooms substrates on the cultivation 3 parameters and fruiting bodies quality. International Biodeterioration and Biodegradation, 64, 638- 645. DOI: 10.1016/j.ibiod.2010.07.003
  • Sabaratnam, V., Kah-hui, W., Naidu, M. & David, P.R. (2011). Neuronal health–Can culinary and medicinal mushrooms help? Journal of traditional and complementary medicine, 3(1), 62-68. DOI: 10.4103/2225-4110.106549
  • Serpen, A., Pelvan, E., Alasalvar, C., Mogol, B.A., Yavuz, H.T., Gökmen, V. & Özçelik, B. (2012). Nutritional and functional characteristics of seven grades of black tea produced in Turkey. Journal of Agricultural Food Chemistry, 60, 7682-7689. DOI: 10.1021/jf302058d
  • Sevindik, M., Bal, C. & Akgül, H. (2018). Comparison of antioxidant potentials of the wild and cultivated forms of edible pleurotus ostreatus and Agaricus bisporus mushrooms, Türk Yaşam Bilimleri Dergisi, 3/2, 263-266.
  • Shirmila, J.G. & Radhamany, P.M. (2013). In vitro antioxidant activities, total phenolics and flavonoid of wild edible mushroom Macrolepiota mastoidea (Fr.) Singer. International Journal of Pharmacy and Pharmaceutical Sciences, 5(2), 161-166.
  • Silva, S.O., Costa, S.M.G. & Clemente, E. (2002). Chemical composition of Pleurotus pulmonarius (Fr.) Quél., substrates and residue after cultivation. Brazilian Archives of Biology and Technology, 45(4), 531-535. DOI: 10.1590/S1516- 89132002000600018
  • Singh, V., Pandey R. & Vyas D. (2015). Antioxidant potentiality of Pleurotus ostreatus (MTCC142) cultivated on different agro wastes. Asian Journal of Plant Science and Research, 5(6), 22-27.
  • Singleton, V.L., Orthofer, R. & Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. In Methods in Enzymology, Oxidant and Antioxidants (Part A); Packer, L., Ed; Academic Press: San Diego, CA, 299, 152-178 p.
  • Slinkard, K. & Singleton, V.L. (1977). Total phenol analysis automation and comparison with manual methods. American Journal of Enology and Viticulture, 28, 49-55.
  • Sturion, G.L. & Oetterer, M. (1995). Composição química de cogumelos comestíveis (Pleurotus spp.) originados de cultivo em diferentes substratos. Ciência e Tecnologia de Alimentos, 15(2), 189-193.
  • Sulistiany, H., Sudirman, L.I. & Dharmaputra, O. S. (2016). Production of fruiting body and antioxidant activity of wild Pleurotus. HAYATI Journal of Biosciences, 23(4), 191-195. DOI: 10.1016 /j.hjb.2016.07.003.
  • Svaprakasam, K. & Kondaswary, T.K. (1981). Waste materials for the cultivation of Pleurotus sajor- caju. The Mushroom Journal, 101, 178-179.
  • Turkoglu, A., Duru, M.E., Mercan, N., Kivrak, I. & Gezer, K. (2007). Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chemistry, 101, 267-73. DOI: 10.1016/j .foodchem.2006.01.025
  • Vamanu, E. (2013). Studies on the antioxidant and antimicrobial activities of Pleurotus ostreatus PSI101109 mycelium. Pakistan Journal of Botany, 45(1), 311-317.
  • Yıldız, S., Yılmaz, A., Can, Z., Kılıç, C. & Yıldız, Ü.C. (2017). Total phenolic, flavonoid, tannin contents and antioxidant properties of Pleurotus ostreatus And Pleurotus citrinopileatus cultıvated on various sawdust. The Journal of Food, 42(3), 315- 323.
  • Yılmaz, A., Yıldız, S. , Tabbouche, S. , Kılıç, A.O. & Can, Z. (2016). Total phenolic content, antioxidant and antimicrobial properties of Pleurotus ostreatus grown on lime Tilia Tomentosa Leaves. Hacettepe Journal of Biology and Chemistry, 44(2), 119-124. DOI: 10.15671/HJBC.20184417585
  • Yılmaz, A., Yıldız, S., Kılıç C. & Can Z. (2017) Total phenolics, flavonoids, tannin contents and antioxidant properties of Pleurotus ostreatus cultivated on different wastes and sawdust. International Journal Secondary Metabolite, 4(1), 1-9. DOI: 10.21448/ijsm.252052
APA BOZDEVECİ A, AVCI S, Alpay Karaoğlu Ş, Can Z, Peksen A (2022). Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?. , 537 - 545. 10.35229/jaes.1180420
Chicago BOZDEVECİ ARİF,AVCI SİBEL,Alpay Karaoğlu Şengül,Can Zehra,Peksen Aysun Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?. (2022): 537 - 545. 10.35229/jaes.1180420
MLA BOZDEVECİ ARİF,AVCI SİBEL,Alpay Karaoğlu Şengül,Can Zehra,Peksen Aysun Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?. , 2022, ss.537 - 545. 10.35229/jaes.1180420
AMA BOZDEVECİ A,AVCI S,Alpay Karaoğlu Ş,Can Z,Peksen A Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?. . 2022; 537 - 545. 10.35229/jaes.1180420
Vancouver BOZDEVECİ A,AVCI S,Alpay Karaoğlu Ş,Can Z,Peksen A Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?. . 2022; 537 - 545. 10.35229/jaes.1180420
IEEE BOZDEVECİ A,AVCI S,Alpay Karaoğlu Ş,Can Z,Peksen A "Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?." , ss.537 - 545, 2022. 10.35229/jaes.1180420
ISNAD BOZDEVECİ, ARİF vd. "Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?". (2022), 537-545. https://doi.org/10.35229/jaes.1180420
APA BOZDEVECİ A, AVCI S, Alpay Karaoğlu Ş, Can Z, Peksen A (2022). Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 7(4), 537 - 545. 10.35229/jaes.1180420
Chicago BOZDEVECİ ARİF,AVCI SİBEL,Alpay Karaoğlu Şengül,Can Zehra,Peksen Aysun Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 7, no.4 (2022): 537 - 545. 10.35229/jaes.1180420
MLA BOZDEVECİ ARİF,AVCI SİBEL,Alpay Karaoğlu Şengül,Can Zehra,Peksen Aysun Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, vol.7, no.4, 2022, ss.537 - 545. 10.35229/jaes.1180420
AMA BOZDEVECİ A,AVCI S,Alpay Karaoğlu Ş,Can Z,Peksen A Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2022; 7(4): 537 - 545. 10.35229/jaes.1180420
Vancouver BOZDEVECİ A,AVCI S,Alpay Karaoğlu Ş,Can Z,Peksen A Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2022; 7(4): 537 - 545. 10.35229/jaes.1180420
IEEE BOZDEVECİ A,AVCI S,Alpay Karaoğlu Ş,Can Z,Peksen A "Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?." JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 7, ss.537 - 545, 2022. 10.35229/jaes.1180420
ISNAD BOZDEVECİ, ARİF vd. "Do Different Substrates Affect Antioxidant Properties and Antimicrobial Activity of Pleurotus ostreatus?". JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 7/4 (2022), 537-545. https://doi.org/10.35229/jaes.1180420