Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris
Yıl: 2023 Cilt: 27 Sayı: 1 Sayfa Aralığı: 64 - 72 Metin Dili: İngilizce DOI: 10.29050/harranziraat.1216304 İndeks Tarihi: 07-04-2023
Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris
Öz: In 2021, post-harvest soil samples were taken from fields where chickpea and wheat were grown in Yozgat province to isolate bacteria. Of 74 isolates obtained from soil samples, 14 nonpathogenic isolates were evaluated for their inhibitory potential against two pathogenic isolates (YBUFoc9 and YBUFoc2) of Fusarium wilt disease agent Fusarium oxysporum f. sp. ciceris. Antagonistic bacterial isolates were identified as Bacillus spp. by Blastn analysis based on their 16S rDNA nucleotide sequences. Among them, 2 isolates were identified as B. amyloliquefaciens (BM23 and BM40), 3 isolates as B. subtilis (BM8, BM32, and BM105), 6 isolates as B. cereus (BM10, BM69, BM70, BM104, BM111 and BM215), 2 isolates as B. megaterium (BM44 and BM135) and 1 isolate as B. pumilus (BM28). No effective isolate of any genus other than Bacillus spp. was found. They inhibited mycelial growth of isolates YBUFoc9 and YBUFoc2 from 0 to 78.50% and from 0 to 58.33%, respectively. Among the 5 different species, B. amyloliquefaciens (BM23 and BM40) and B. subtilis (BM8, BM32, and BM105) showed the strongest inhibitory activity against the two fungal isolates under in vitro conditions. In further studies, the biocontrol properties of the most effective isolates (BM23, BM40, BM8, BM32 and BM105) will be investigated and compared with approved fungicides for their fungicidal activity.
Anahtar Kelime: Toprak örneklerinden bakteri izolatlarının izolasyonu ve nohut solgunluğu hastalığına neden olan Fusarium oxysporum f. sp. ciceris’e karşı in vitro antagonistik potansiyellerinin belirlenmesi
Öz: 2021 yılında Yozgat ilinde nohut ve buğday yetiştirilen tarlalardan bakteri izolasyonu için hasat sonrası toprak örnekleri toplanmıştır. Toprak örneklerinden izole edilen 74 izolat arasından patojenik olmayan 14 aday bakteri izolatın Fusarium solgunluğu etmeni Fusarium oxysporum f. sp. ciceris’in iki izolatına (YBUFoc9 and YBUFoc2) karşı engelleme potansiyelleri in vitro koşullarda değerlendirilmiştir. Antagonist bakteri izolatların tanısı 16S rDNA nükleotid dizilerine dayanan Blastn analizi ile Bacillus spp. olarak belirlenmiştir. Elde edilen izolatların 2'si B. amyloliquefaciens (BM23 ve BM40), 3'ü B. subtilis (BM8, BM32 ve BM105), 6'sı B. cereus (BM10, BM69, BM70, BM104, BM111 ve BM215), 2’si B. megaterium olarak (BM44 ve BM135) ve 1’i B. pumilus (BM28) olarak tanılanmıştır. Antagonistik etkinlik bakımından Bacillus spp. dışında etkili bir cinse rastlanılmamıştır. Bakteriyel izolatlar, YBUFoc9 ve YBUFoc2 izolatlarının misel büyümesine sırasıyla %0-%78.50 ve %0-%58.33 oranında inhibisyon sağlamışlardır. Beş farklı tür arasından B. amyloliquefaciens (BM23 ve BM40) ve B. subtilis’in (BM8, BM32 ve BM105) in vitro koşullar altında patojenin iki izolatına karşı en güçlü inhibisyon aktivitesi gösterdiği belirlenmiştir. Gelecekte yapılması planlanan çalışmalarda, en etkili izolatların (BM23, BM40, BM8, BM32 ve BM105) biyokontrol özellikleri araştırılacak ve fungisidal aktiviteleri için onaylanmış fungisitlerle karşılaştırılacaktır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ozturk m, ENDES A (2023). Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. , 64 - 72. 10.29050/harranziraat.1216304 |
| Chicago | ozturk murat,ENDES Ali Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. (2023): 64 - 72. 10.29050/harranziraat.1216304 |
| MLA | ozturk murat,ENDES Ali Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. , 2023, ss.64 - 72. 10.29050/harranziraat.1216304 |
| AMA | ozturk m,ENDES A Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. . 2023; 64 - 72. 10.29050/harranziraat.1216304 |
| Vancouver | ozturk m,ENDES A Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. . 2023; 64 - 72. 10.29050/harranziraat.1216304 |
| IEEE | ozturk m,ENDES A "Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris." , ss.64 - 72, 2023. 10.29050/harranziraat.1216304 |
| ISNAD | ozturk, murat - ENDES, Ali. "Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris". (2023), 64-72. https://doi.org/10.29050/harranziraat.1216304 |
| APA | ozturk m, ENDES A (2023). Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. Harran Tarım ve Gıda Bilimleri Dergisi, 27(1), 64 - 72. 10.29050/harranziraat.1216304 |
| Chicago | ozturk murat,ENDES Ali Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. Harran Tarım ve Gıda Bilimleri Dergisi 27, no.1 (2023): 64 - 72. 10.29050/harranziraat.1216304 |
| MLA | ozturk murat,ENDES Ali Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. Harran Tarım ve Gıda Bilimleri Dergisi, vol.27, no.1, 2023, ss.64 - 72. 10.29050/harranziraat.1216304 |
| AMA | ozturk m,ENDES A Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. Harran Tarım ve Gıda Bilimleri Dergisi. 2023; 27(1): 64 - 72. 10.29050/harranziraat.1216304 |
| Vancouver | ozturk m,ENDES A Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. Harran Tarım ve Gıda Bilimleri Dergisi. 2023; 27(1): 64 - 72. 10.29050/harranziraat.1216304 |
| IEEE | ozturk m,ENDES A "Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris." Harran Tarım ve Gıda Bilimleri Dergisi, 27, ss.64 - 72, 2023. 10.29050/harranziraat.1216304 |
| ISNAD | ozturk, murat - ENDES, Ali. "Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris". Harran Tarım ve Gıda Bilimleri Dergisi 27/1 (2023), 64-72. https://doi.org/10.29050/harranziraat.1216304 |
