Objective: Premenstrual syndrome (PMS) is a combination of behavioral, somatic, emotional, and cognitive symptoms that are very common in women during reproductive age. The worldwide prevalence of PMS varies between 12% and 98%. Thus, this study aimed to determine the prevalence of PMS and its associated factors and comfort level in a group of university students who stay in a dormitory. Methods: This cross-sectional study was conducted in Yozgat Sürmeli Girls’ Dormitory, which is located within Yozgat Bozok University Erdoğan Akdağcampus. A total of 1162 students are staying in the dormitory, and the study was completed with 935 students who stayed in the dormitory and volunteered to participate in the study without sample selection. The study collected data using the Participant Information Form that consist of 30 questions prepared by the researchers, the premenstrual syndrome scale (PMSS), and the general comfort scale (GCS). Percentage, mean, chi-square, t-test, correlation, and logistic regression analysis were used for data evaluation. Results: The mean age of the students was 20.81±1.487 years. The total score of the PMS scale was 115.21±41.615. On the scale, the highest score is 20.203±7.493 from the depressive affection dimension and the lowest score is 7.854±3.771 from the sleep change dimension. PMS was found in 34.2% of students. The total GCS score of the students was 2.43±0.35, and scores obtained from the sub-dimensions and GCS levels were lower in students with PMS. A weak and negative relationship was found between the PMSS and the GCS. The risk of PMS increased by 1.366 times (p=0.036) in students with menarche at the age of 13 years and younger, whereas, in students with chronic diseases, it was 2.512 times higher (p=0.001). The use of salt without tasting the food and daily coffee consumption increased the risk of PMS by 1.626 times (p=0.004) and 1.882 times (p=0.000), respectively. The risk of PMS was 2.026 times (p=0.000) higher in students with dysmenorrhea, whereas 1.614 times higher in students who used any method to cope with dysmenorrhea (p=0.004). Conclusion: PMS is an important problem among women. A weak and negative relationship was found between PMS and comfort level. Considering the results of this study, menarche before the age of 13, the presence of chronic disease, dysmenorrhea, excessive salt use, and coffee consumption increases the risk of PMS. Many factors lead to the occurrence of PMS. Interventional studies are necessary to reduce the risk factors for PMS that significantly affect the women’s health or its risk factors.