The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study

Cebrail AKYUZ, (Haydarpaşa Eğitim ve Araştırma Hastanesi, Genel Cerrahi Kliniği, İstanbul, Türkiye)
Emine ERDAG, (Yakın Doğu Üniversitesi, Eczacılık Fakültesi, Farmasötik Kimya Anabilim Dalı, Mersin, Türkiye)
Ayliz Velioglu-OGUNC, (Marmara Üniversitesi, Sağlık Meslek Yüksekokulu, Biyokimya Anabilim Dalı, İstanbul, Türkiye)
Nurettin ABACIOGLU, (Yakın Doğu Üniversitesi, Eczacılık Fakültesi, Eczacılık Anabilim Dalı,, Mersin, Türkiye)
Ahmet Ozer SEHIRLI (Yakın Doğu Üniversitesi, Diş Hekimliği Fakültesi, Farmakoloji Anabilim Dalı, Mersin, Türkiye)
Journal of research in pharmacy (online)
6 0
Yıl: 2023 Cilt: 27 Sayı: 4 Sayfa Aralığı: 1513 - 1523 Metin Dili: İngilizce DOI: 10.29228/jrp.437 İndeks Tarihi: 28-07-2023

The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study

Öz:
In this study, we aimed to determine the protective effect of grape seed extract against mesenteric ischemia/reperfusion (I/R) injury in rats. In addition, an in silico molecular docking study was performed to compare the effects of natural compounds in grape seed extract on myeloperoxidase (MPO) enzyme at the molecular level. In addition to MPO activity, malondialdehyde (MDA) and glutathione (GSH) activities were also measured in the intestinal tissue samples. Serum cytokines, TNF-α and IL-1β, were measured to assess systemic tissue damage. Mesenteric GSH decreased significantly after ischemia/reperfusion, while MDA levels and MPO activity increased significantly. Binding interactions were detected by the binding mode of analysis, and the study compared the binding affinities of compounds at the active site of myeloperoxidase and glutathione peroxidase. As a result, it was found that procyanidins (B1-B4), which are predominantly present in GS extract, have the highest binding affinity and molecular interaction.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA AKYUZ C, ERDAG E, Velioglu-OGUNC A, ABACIOGLU N, SEHIRLI A (2023). The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. , 1513 - 1523. 10.29228/jrp.437
Chicago AKYUZ Cebrail,ERDAG Emine,Velioglu-OGUNC Ayliz,ABACIOGLU Nurettin,SEHIRLI Ahmet Ozer The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. (2023): 1513 - 1523. 10.29228/jrp.437
MLA AKYUZ Cebrail,ERDAG Emine,Velioglu-OGUNC Ayliz,ABACIOGLU Nurettin,SEHIRLI Ahmet Ozer The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. , 2023, ss.1513 - 1523. 10.29228/jrp.437
AMA AKYUZ C,ERDAG E,Velioglu-OGUNC A,ABACIOGLU N,SEHIRLI A The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. . 2023; 1513 - 1523. 10.29228/jrp.437
Vancouver AKYUZ C,ERDAG E,Velioglu-OGUNC A,ABACIOGLU N,SEHIRLI A The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. . 2023; 1513 - 1523. 10.29228/jrp.437
IEEE AKYUZ C,ERDAG E,Velioglu-OGUNC A,ABACIOGLU N,SEHIRLI A "The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study." , ss.1513 - 1523, 2023. 10.29228/jrp.437
ISNAD AKYUZ, Cebrail vd. "The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study". (2023), 1513-1523. https://doi.org/10.29228/jrp.437
APA AKYUZ C, ERDAG E, Velioglu-OGUNC A, ABACIOGLU N, SEHIRLI A (2023). The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. Journal of research in pharmacy (online), 27(4), 1513 - 1523. 10.29228/jrp.437
Chicago AKYUZ Cebrail,ERDAG Emine,Velioglu-OGUNC Ayliz,ABACIOGLU Nurettin,SEHIRLI Ahmet Ozer The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. Journal of research in pharmacy (online) 27, no.4 (2023): 1513 - 1523. 10.29228/jrp.437
MLA AKYUZ Cebrail,ERDAG Emine,Velioglu-OGUNC Ayliz,ABACIOGLU Nurettin,SEHIRLI Ahmet Ozer The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. Journal of research in pharmacy (online), vol.27, no.4, 2023, ss.1513 - 1523. 10.29228/jrp.437
AMA AKYUZ C,ERDAG E,Velioglu-OGUNC A,ABACIOGLU N,SEHIRLI A The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. Journal of research in pharmacy (online). 2023; 27(4): 1513 - 1523. 10.29228/jrp.437
Vancouver AKYUZ C,ERDAG E,Velioglu-OGUNC A,ABACIOGLU N,SEHIRLI A The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. Journal of research in pharmacy (online). 2023; 27(4): 1513 - 1523. 10.29228/jrp.437
IEEE AKYUZ C,ERDAG E,Velioglu-OGUNC A,ABACIOGLU N,SEHIRLI A "The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study." Journal of research in pharmacy (online), 27, ss.1513 - 1523, 2023. 10.29228/jrp.437
ISNAD AKYUZ, Cebrail vd. "The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study". Journal of research in pharmacy (online) 27/4 (2023), 1513-1523. https://doi.org/10.29228/jrp.437
Sayfa Oluşturulma Tarihi
24-04-2024 8:38

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