Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis
Yıl: 2023 Cilt: 48 Sayı: 2 Sayfa Aralığı: 295 - 302 Metin Dili: İngilizce DOI: 10.55262/fabadeczacilik.1324184 İndeks Tarihi: 17-08-2023
Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis
Öz: Cystic Echinococcosis (CE) is a type of zoonotic infection that can be caused by a specific form of a parasite called Echinococcus granulosus sensu lato. Mainly, imaging techniques are utilized to diagnose CE. Serological tests are only used when imaging findings are atypical. Additionally, laboratory assays including, direct microscopy and PCR are used to confirm of diagnosis after treatment though obtained negative results with these tests cannot be ruled out the diagnosis. Specific miRNAs produced by the parasite could be used as markers to diagnose and monitor CE. This research investigates the diagnostic potential of parasite-derived miRNAs compared to the presence of protoscolex in animal-derived hydatid cyst samples. Accordingly, egr-let-7-5p, egr-miR71-5p, and egr-miR-9-5p were positive in 26, 25, and 11 out of 30 samples (86.6%, 83.3%, and (36.6%), respectively. There was no relationship between protoscolex presence and detection of either egr-let-7-5p or egr-miR-9-5p (p>0.05). On the other hand, egr-miR71-5p positivity was found to be statistically significant compared with protoscolex presence (p=0.04). As a result, egr-miR-71 is a promising potential target for the diagnosis of CE. Additional research is necessary to assess the diagnostic value of miRNAs in CE using a larger group of samples.
Anahtar Kelime: Parazit Kaynaklı MikroRNA’lar: Kistik Ekinokokkoz’un Laboratuvar Tanısında Potansiyel Alternatif Hedefler
Öz: Kistik Ekinokokkoz (KE), Echinococcus granulosus sensu lato adlı parazitin larva formunun neden olduğu bir tür zoonotik enfeksiyondur. Çoğunlukla, KE tanısı için görüntüleme teknikleri kullanılmaktadır. Serolojik testler yalnızca görüntüleme bulguları atipik olduğunda tanıya yardımcı olmakta, tedavi sonrası tanının doğrulanmasında direkt mikroskopi ve PCR gibi laboratuvar tetkikleri de kullanılmaktadır. Ancak bu testlerle elde edilen negatif sonuçlar tanıyı dışlamamaktadır. Parazit tarafından üretilen spesifik mikroRNA’lar (miRNA), KE’yi teşhis etmek ve hastaları tedavi etkinliği açısından takip için belirteçler olarak kullanım potansiyeli taşımaktadır. Bu araştırmanın amacı, hayvan kaynaklı hidatik kist örneklerinde protoskoleks varlığına kıyasla parazit kaynaklı miRNA’ların teşhis potansiyelini araştırmaktır. Buna göre egr- let-7-5p, egr-miR71-5p ve egr-miR-9-5p, 30 örneğin sırasıyla 26, 25 ve 11’inde (%86,6, %83,3 ve %36,6) pozitif bulunmuştur. Protoskoleks varlığı ile egr-let-7-5p veya egr-miR-9-5p’nin saptanması arasında anlamlı bir ilişki (p>0.05) olmamasına karşın egr-miR71-5p pozitifliği ise protoskoleks varlığına göre istatistiksel olarak anlamlı bulunmuştur (p=0,04). Sonuç olarak, egr-miR-71, KE tanısı için umut verici bir potansiyel hedef olarak görülmektedir. İleri çalışmalarda, daha büyük bir örneklem kullanarak KE tanısında parazit kaynaklı miRNA’ların tanısal değerinin değerlendirilmesi gereklidir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Örsten S, Baysal i, MAÇIN S (2023). Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis. , 295 - 302. 10.55262/fabadeczacilik.1324184 |
| Chicago | Örsten Serra,Baysal ipek,MAÇIN SALIH Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis. (2023): 295 - 302. 10.55262/fabadeczacilik.1324184 |
| MLA | Örsten Serra,Baysal ipek,MAÇIN SALIH Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis. , 2023, ss.295 - 302. 10.55262/fabadeczacilik.1324184 |
| AMA | Örsten S,Baysal i,MAÇIN S Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis. . 2023; 295 - 302. 10.55262/fabadeczacilik.1324184 |
| Vancouver | Örsten S,Baysal i,MAÇIN S Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis. . 2023; 295 - 302. 10.55262/fabadeczacilik.1324184 |
| IEEE | Örsten S,Baysal i,MAÇIN S "Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis." , ss.295 - 302, 2023. 10.55262/fabadeczacilik.1324184 |
| ISNAD | Örsten, Serra vd. "Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis". (2023), 295-302. https://doi.org/10.55262/fabadeczacilik.1324184 |
| APA | Örsten S, Baysal i, MAÇIN S (2023). Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis. FABAD Journal of Pharmaceutical Sciences, 48(2), 295 - 302. 10.55262/fabadeczacilik.1324184 |
| Chicago | Örsten Serra,Baysal ipek,MAÇIN SALIH Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis. FABAD Journal of Pharmaceutical Sciences 48, no.2 (2023): 295 - 302. 10.55262/fabadeczacilik.1324184 |
| MLA | Örsten Serra,Baysal ipek,MAÇIN SALIH Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis. FABAD Journal of Pharmaceutical Sciences, vol.48, no.2, 2023, ss.295 - 302. 10.55262/fabadeczacilik.1324184 |
| AMA | Örsten S,Baysal i,MAÇIN S Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis. FABAD Journal of Pharmaceutical Sciences. 2023; 48(2): 295 - 302. 10.55262/fabadeczacilik.1324184 |
| Vancouver | Örsten S,Baysal i,MAÇIN S Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis. FABAD Journal of Pharmaceutical Sciences. 2023; 48(2): 295 - 302. 10.55262/fabadeczacilik.1324184 |
| IEEE | Örsten S,Baysal i,MAÇIN S "Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis." FABAD Journal of Pharmaceutical Sciences, 48, ss.295 - 302, 2023. 10.55262/fabadeczacilik.1324184 |
| ISNAD | Örsten, Serra vd. "Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis". FABAD Journal of Pharmaceutical Sciences 48/2 (2023), 295-302. https://doi.org/10.55262/fabadeczacilik.1324184 |
