Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis

OK, Mahmut; şen, ismail; ER, CENK; ÇÖL, Ramazan; YILDIZ, Ramazan; GÜZELBEKTEŞ, Hasan; AYDOĞDU, Uğur

Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis

Mahmut OK, (Selçuk Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Konya, Türkiye)

Cenk ER, (Selçuk Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Konya, Türkiye)

Ramazan YILDIZ, (Mehmet Akif Ersoy Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Burdur, Türkiye)

Ramazan ÇÖL, (Selçuk Üniversitesi, Veteriner Fakültesi, Fizyoloji Anabilim Dalı, Konya, Türkiye)

Uğur AYDOĞDU, (Cumhuriyet Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Sivas, Türkiye)

İsmail ŞEN, (Selçuk Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Konya, Türkiye)

Hasan GÜZELBEKTEŞ (Selçuk Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Konya, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

7 3

Yıl: 2015 Cilt: 21 Sayı: 5 Sayfa Aralığı: 761 - 766 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis

Öz:

Bu çalışmanın amacı; sepsisli köpeklerde akut faz proteinler, bazı sitokinler ve hemostatik sistem parametrelerin değişimlerini değerlendirerek,hastalığın tanısında bu parametrelerin önemini ortaya koymaktır. Bu çalışmanın materyalini 30 sepsisli ve 9 sağlıklı köpek oluşturdu. Sepsisliköpeklerde iştahsızlık, durgunluk, depresyon, vücut ısısında artış, mukoz membranlarda konjesyon, kapiller dolum zamanında uzama,taşikardi, takipnea, lökositozis veya lökopeni belirlendi. Bütün köpeklerin interlökin-1ß (IL-1ß), tümör nekroz faktör α (TNF α), interferon γ (INF-γ), C-reaktif protein (CRP), serum amiloid A (SAA) ve protrombin zamanı (PT), aktive edilmiş parsiyel tromboplastin zamanı (aPTT), antitrombinIII (AT III), fibrinojen, protein C ve D-dimer seviyeleri ölçüldü. Sepsisli köpeklerde serum IL-1ß, TNF-α, INF-γ, CRP ve SAA düzeylerinde önemliartış belirlendi. Sepsisli köpeklerde plazma PT ve APTT sürelerinde önemli uzama, fibrinojen, D-dimer ve protein C düzeylerinde önemli artış,AT-III düzeyinde ise önemli azalma tespit edildi. Sonuç olarak sepsisli köpeklerde SAA, IL-1ß ve TNFα paramterelerinin yangısel olaylardaönemli rol aldığı belirlendi. Sepsisli köpeklerde tespit edilen hemostatik anormallikler dissemine intravaskuler koagulasyon (DİK) gelişimiile ilgili olabilir.

Anahtar Kelime:

Konular: Veterinerlik

Sepsisli Köpeklerde Akut Faz Proteinler, Bazı Sitokinler ve Hemostatik Parametrelerin Değerlendirilmesi

Öz:

The aim of this study was to evaluate the alterations in acute phase proteins, cytokines and hemostatic parameters in dogs with sepsis and todetermine the importance of these parameters in diagnosis of the sepsis. Thirty dogs with sepsis and 9 healthy dogs were used in this study.Anorexia, depression, lethargy, hyperthermia, tachycardia, tachypnea, congestion in the mucosal membranes, prolonged capillary refill time,and leukocytosis or leucopenia were identified in the dogs with sepsis. The serum interleukin-1ß (IL-1ß), tumor necrosis factor α (TNF-α),interferon γ (INF-γ), C-reactive protein (CRP), serum amyloid A (SAA), prothrombin time (PT), activated partial thromboplastin time (aPTT),antithrombin III (AT III), fibrinogen, protein C (PC), and D-dimer levels were measured in all dogs. We found that the serum IL-1ß, TNF-α, INF-γ,CRP and SAA concentrations were significantly elevated in dogs with sepsis as compared with healthy controls. In addition, the plasmaPT and APTT levels were notably prolonged, the plasma fibrinogen, D-dimers and protein C concentrations were significantly increased.However, the antithrombin III activity was significantly decreased in the dogs with sepsis. In conclusion, the results of this study indicate thatthe SAA, IL-1ß and TNF-α parameters play important roles in the inflammatory process in dogs with sepsis. The hemostatic abnormalitiesobserved in dogs with sepsis may be due to the development of disseminated intravascular coagulation (DIC).

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. de Boer JP, Wolbink GJ, Thijs LG, Baars JW, Wagstaff J, Hack E: Interplay of complement and cytokines in the pothohenesis of septic shock. Immunopharmacol, 24, 135- 148, 1992. DOI: 10.10 16/0 162- 3109(92)90019-9
2. de Laforcade AM, Freeman LMS, Shaw SP, Brooks MB, Rozanski EA, Rush JE: Hemostatic changes in dogs with naturally occuring sepsis. Vet Intern Med, 17, 674-679, 2003. DOI: 10.1111/j.1939-1676.2003.tb02499.x
3. Nemzek JA, Agrodnia MD, Hauptman G: Breed-specific pro- inflammatory cytokine production as a predisposing factor for susceptibility in the dogs. Vet Emerg Crit Care, 17, 368-372, 2007. DOI: 10.1111/j.1476-4431.2006.00215.x
4. de Laforcade AM, Rozanski EA, Freeman LM, Li W: Serial evalutaion of protein C and antitrombin in dogs with sepsis. J Vet Intern Med, 22, 26- 30, 2008. DOI: 10.1111/j.1939-1676.2007.0021.x
5. Otto CM, Rieser TM, Brooks MB, Russell MW: Evidence of hyper- coagulability in dogs with parvoviral enteritis. JAVMA, 217, 1500-1509, 2000. DOI: 10.2460/javma.2000.217.1500
6. Rau S, Kohn B, Richter C, Fenske N, Kuchenhoff H, Hartmann K, Hartle S, Kaspers B, Hirschberger J: Plasma interleukin-6 response is predictive for severity and mortality in canine systemic inflammatory response syndrome and sepsis. Vet Clin Pathol, 36, 253-260, 2007. DOI: 10.1111/j.1939-165X.2007.tb00220.x
7. Song R, Kim J, Yu D, Park C, Park J: Kinetics of IL-6 and TNF-α changes in canine models of sepsis induced by endotoxin. Vet Immunol Immunopathol, 146, 143-149, 2012. DOI: 10.1016/j.vetimm.2012.02.008
8. Bochsler PN, Slauson DO: Inflammation and repair tissue. In, Mechanism of Disease. A Textbook of Comparetive General Pathology. Third ed., 140-245, Mosby, St. Louis, 2002.
9. Cary C: Acute phase proteins in animals. Prog Mol Biol Transl Sci, 105, 113-150, 2011. DOI: 10.1016/B978-0-12-394596-9.00005-6
10. Jain S, Gautam V, Naseem S: Acute-phase proteins: As daignostic tool. J Pram Bioall Sci, 1, 118-127, 2011. DOI: 10.4103/0975-7406.76489
11. Kjelgaard-Hansen M, Jacobsen S: Assay validation and diagnostic applications of major acute-phase proteins testing in companion animals. Clin Lab Med, 31, 51-70, 2011. DOI: 10.1016/j.cll.2010.10.002
12. Kocaturk M, Martinez S, Eralp O, Tvarijonaviciute A, Ceron J, Yilmaz Z: Prognostic value of serum acute-phase proteins in dogs with parvoviral enteritis. J Small Anim Pract, 51, 478-483, 2010. DOI: 10.1111/ j.1748-5827.2010.00965.x
13. Murata H, Shimada N, Yoshioka M: Current research on acute phase proteins in veterinary diagnosis: An overview. Vet J, 168, 28-40, 2004. DOI: 10.1016/S1090-0233(03)00119-9
14. Turgut K: Veteriner Klinik Laboratuvar Teşhis. İkinci Baskı, Bahçıvanlar Basım Sanayi A.Ş. Konya, s.235-245, 2000.
15. Cary C: Biomarkers of inflammation in exotic pets. J Exotic Pet Med, 22, 245-250, 2013. DOI: 10.1053/j.jepm.2013.08.003
16. Gebhardt C, Hirschberger J, Rau S, Arndt G, Krainer K, Schweigert FJ, Brunnberg L, Kaspers B, Kohn B: Use of C-reaktive protein to predict outcome in dogs with systemic inflammatory response syndrome or sepsis. Vet Emerg Crit Care, 19, 450-458, 2009. DOI: 10.1111/j.1476- 4431.2009.00462.x
17. Langhorn CMB, Goddard R, Andreasen A, Moldal E, Tvarijonaviciute A, Kirpensteij n J, Jakobsen S, Persson F, Kj elgaard-Hansen M: Comparison of serum amyloid A and C-reactive protein as diagnostic markers of systemic inflammation in dogs. Can Vet J, 55, 161-168, 2014.
18. Jitpean S, Holst BS, Höglund OV, Pettersson A, Olsson U, Strage E, Södersten F, Hagman R: Serum insulin-like factor-I, iron, C-reactive protein, and serum amyloid A for prediction of outcome in dogs with pyometra. Theriogenelogy, 30, 1-6, 2014. DOI: 10.1016/j.theriogenology. 2014.02.014
19. Bruchim Y, Aroch I, Saragusty J, Waner T: Disseminated intravascular coagulation. Compendium, 3, 1-16, 2008. DOI: 10.1056/ NEJM199908193410807
20. Caldin M, Furlanello T, Lubas G: Validation of an immunoturbidimetric D-dimer assay in canine citrated plasma. Vet Clin Pathol, 29, 51-54, 2000. DOI: 10.1111/j.1939-165X.2000.tb00398.x
21. Esmon CT: Inflammation and thrombosis. J Thromb Haemost, 1, 1343-1348, 2003. DOI: 10.1046/j.1538-7836.2003.00261.x
22. Ceremi A: Inflammatory cytokines. Clin Immunol Immunolpathol, 62, 3-10, 1999.
23. Alsemgeest SP, Van- Klooster GA, Van Miert AS, Hulskamp-Koch CK, Gruys E: Primary bovine hepatocytes in the study of cytokine induced acute-phase protein secretion in vitro. Vet Immunol Immunopathol, 53, 179-184, 1996. DOI: 10.1016/0165-2427(96)05602-4
24. Baggio V, Ott F, Fischer RW: Production of antibodies to canine IL- 1ß and canine TNF to assess the role of proinflammatory cytokines. Vet Immunol Immunopathol, 107, 27-39, 2005.
25. Nakagawa-Tosa N, Morimatsu M, Kawasaki MN, Nakatsuji H, Syuto B, Saito M: Stimulation of haptoglobin synthesis by interleukin-6 and tumor necrosis factor, but not by interleukin-1, in bovine primary cultured hepatocytes. J Vet Med Sci, 57, 219-223, 1995. DOI: 10.1292/jvms.57.219
26. Ohtsuka H, Ohki K, Tanaka T, Tajima M, Yoshino T, Takahashi K: Circuling tumor necrosis factor and interleukin-1 after administration of lps in adult cows. J Vet Med Sci, 19, 227-229, 1997. DOI: 10.1292/ jvms.59.927
27. Nemzek JA, Siddiqui J, Remick DG: Development and optimization of cytokine ELISAs using commercial antibody pairs. J Immunol Methods, 255, 149-157, 2001. DOI: 10.1016/S0022-1759(01)00419-7
28. Grone A, Aldinger S, Baumgartner W: Interleukin-1beta-6-12 and tumor necrosis factor-alpha experission in barin of dogs with canine distemper virus infection. J Neuroimmunol, 110, 20-30, 2000. DOI: 10.1016/S0165-5728(00)00332-5
29. Miyamota T, Fujinaga T, Yamashita K, Hagio M: Changes of serum cytokine activities and other parameters in dogs with experimentally induced endotoxic shock. Jpn J Vet Res, 44, 107-118, 1996. DOI: 10.14943/ jjvr.44.2.107
30. Fransson BA, Lagerstedt AS, Bergstrom A, Hagman R, Park JS, Evans MA, Ragle CA: C-reaktive protein, tumor necrosis factor α and interleukin-6 in dogs with pyometra and SIRS. Vet Emerg Crit Care, 17, 373- 381, 2007. DOI: 10.1111/j.1476-4431.2006.00203.x
31. Ceron JJ, Eckersall PD, Martinez-Subiela S: Acute phase proteins in dogs and cats: Current knowledge and future perspectives. Vet Clinical Pathol, 34, 85-99, 2005. DOI: 10.1111/j.1939-165X.2005.tb00019.x
32. Eckersall PD, Bell R: Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. Vet J, 185, 23-27, 2010. DOI: 10.1016/j.tvj l.2010.04.009
33. Yule TD, Roth MB, Dreier K, Johnson AF, Palmer-Densmore M, Simmons K, Fanton R: Canine parvovirus vaccine elicits protection from the inflammatory clinical consequences of the disease. Vaccine, 15, 720- 729, 1997. DOI: 10.1016/S0264-410X(96)00232-0
34. Mastrorilli C, Dondi F, Agnoli C: Clinopathologic features and outcome predictors of leptospira interogans Australis serogroup infection in dogs: A retrospective study of 20 cases (2001-2004). J Vet Intern Med, 21, 3-10, 2007. DOI: 10.1892/0891-6640(2007)21[3:CFAOPO]2.0.CO;2
35. Mylonakis ME, Ceron JJ, Leontides L, Siarkou VI, Martinez S, Tvarijonaviciute A, Koutinas AF, Harrus S: Serum acute phase proteins as clinical phase indicators and outcome predicators in naturally occuring canine monocytic ehrlichiosis. J Vet Intern Med, 25, 811-817, 2011. DOI: 10.1111/j.1939-1676.2011.0728.x
36. Saba HI, Morelli GA: T he pathogenesis and management of disseminated intravascular coagulation. Clin Adv Hematol Oncol, 4, 919- 926, 2006.
37. Col R, Durgun Z: Sepsis, lökositler, sitokinler ve disseminant intra- vasküler koagulasyon. Vet Bil Derg, 23, 97-106, 2007.
38. Goddard A, Leisewitz AL: Canine parvovirus. Vet Clin North Am: Small Anim Pract, 40, 1041-1053, 2010. DOI: 10.1016/j.cvsm.2010.07.007
39. Levi M: Pathogenesis and treatment of DIC. Thromb Res, 1, 54-55, 2005. 40. Mischke R: Acute haemostatic changes in accidentally traumatised dogs. Vet J, 169, 60-64, 2005. DOI: 10.1016/j.tvj l.2004.01.008
41. Feldman BF, Madewell BR, ONeill S: Disseminated intravascular coagulation: Antitrombin, plasminogen, and coagulation abnormalities in 41 dogs. JAVMA, 179, 151-154, 1981.
42. Yilmaz Z, Yalçın E, Ilçol Y: Septik şoklu köpeklerde koagulasyon profilinin değerlendirilmesi. Turk J Vet Anim Sci, 26, 171-176, 2002.
43. Bauer N, Moritz A: Coagulation response in dogs with and without systemic inflammatory response syndrome-Preliminary results. Res Vet Sci, 94, 123-121, 2013. DOI: 10.1016/j.rvsc.2012.07.029
44. Bentley AM, Mayhew PD, Culp WTN, Otto CM: Alterations in the hemostatic profiles of dogs with naturally occuring septic peritonitis. Vet Emerg Crit Care, 23, 14-22, 2013. DOI: 10.1111/vec.12013
45. Prins M, Schellens CJMM, van Leeuwen MW, Rothuizen J, Teske E: Coagulation disorders in dogs with hepatic disease. Vet J, 185, 163-168, 2010. DOI: 10.1016/j.tvj l.2009.05.009
46. Er C, Ok M: Levels of cardiac biomarkers and coagulation profiles in dogs with parvoviral enteritis. Kafkas Univ Vet Fak Derg, 21, 383-388, 2015. DOI: 10.9775/kvfd.2014.12575
47. Otto CM: Sepsis. In, Wingfield WE (Ed): The Veterinary ICU Book. 695- 709, Jackson Hole, WY: Teton NewMedia, 2002.
48. Wada H: Disseminated intravascular coagulation. Clin Chim Acta, 344, 13-21, 2004. DOI: 10.1016/j.cccn.2004.02.015
49. Yan SB, Dhainaut JF: Activated protein C versus protein C in severe sepsis. Crit Care Med, 29 (Suppl. 7): S69-S74, 2001.
50. Fourrier F, Goudemand J, Hendrycx S, Caron C, Rime A, Marey A, Lesteval P: Septic shock, multiple organ failure, and disseminated intravascular coagulation. Chest, 101, 816-823, 1992. DOI: 10.1378/ chest.101.3.816

APA	OK M, ER C, YILDIZ R, ÇÖL R, AYDOĞDU U, şen i, GÜZELBEKTEŞ H (2015). Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis. , 761 - 766.
Chicago	OK Mahmut,ER CENK,YILDIZ Ramazan,ÇÖL Ramazan,AYDOĞDU Uğur,şen ismail,GÜZELBEKTEŞ Hasan Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis. (2015): 761 - 766.
MLA	OK Mahmut,ER CENK,YILDIZ Ramazan,ÇÖL Ramazan,AYDOĞDU Uğur,şen ismail,GÜZELBEKTEŞ Hasan Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis. , 2015, ss.761 - 766.
AMA	OK M,ER C,YILDIZ R,ÇÖL R,AYDOĞDU U,şen i,GÜZELBEKTEŞ H Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis. . 2015; 761 - 766.
Vancouver	OK M,ER C,YILDIZ R,ÇÖL R,AYDOĞDU U,şen i,GÜZELBEKTEŞ H Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis. . 2015; 761 - 766.
IEEE	OK M,ER C,YILDIZ R,ÇÖL R,AYDOĞDU U,şen i,GÜZELBEKTEŞ H "Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis." , ss.761 - 766, 2015.
ISNAD	OK, Mahmut vd. "Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis". (2015), 761-766.

APA	OK M, ER C, YILDIZ R, ÇÖL R, AYDOĞDU U, şen i, GÜZELBEKTEŞ H (2015). Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(5), 761 - 766.
Chicago	OK Mahmut,ER CENK,YILDIZ Ramazan,ÇÖL Ramazan,AYDOĞDU Uğur,şen ismail,GÜZELBEKTEŞ Hasan Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, no.5 (2015): 761 - 766.
MLA	OK Mahmut,ER CENK,YILDIZ Ramazan,ÇÖL Ramazan,AYDOĞDU Uğur,şen ismail,GÜZELBEKTEŞ Hasan Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.21, no.5, 2015, ss.761 - 766.
AMA	OK M,ER C,YILDIZ R,ÇÖL R,AYDOĞDU U,şen i,GÜZELBEKTEŞ H Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(5): 761 - 766.
Vancouver	OK M,ER C,YILDIZ R,ÇÖL R,AYDOĞDU U,şen i,GÜZELBEKTEŞ H Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(5): 761 - 766.
IEEE	OK M,ER C,YILDIZ R,ÇÖL R,AYDOĞDU U,şen i,GÜZELBEKTEŞ H "Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21, ss.761 - 766, 2015.
ISNAD	OK, Mahmut vd. "Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21/5 (2015), 761-766.