Kronik Lenfositik Lösemide Risk Faktörü Olarak Endotelyal Nitrik Oksit Sentaz Gen Varyantlari

Yıl: 2017 Cilt: 27 Sayı: 2 Sayfa Aralığı: 85 - 90 Metin Dili: Türkçe
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Kronik Lenfositik Lösemide Risk Faktörü Olarak Endotelyal Nitrik Oksit Sentaz Gen Varyantlari

Öz:
Nitrik oksit (NO) karsinogenezde karışık role sahiptir. Endotelial nitrik oksit sentaz (eNOS) geni üretilen NO'un çoğundan sorumludur. Bu nedenle, eNOS gen varyantlarının kansere yatkınlık ile ilişkili olduğu varsayılmaktadır. Bu çalışmanın amacı kronik lenfositik lösemili (KLL) Türk hastalarında eNOS (G894T ve intron 4 VNTR a/b) varyantlarının risk faktörü olup olmadığı saptamaktır. Bu çalışma, KLL hastalarında prospektif, tek merkezli kesitsel bir çalışmadır. Bu çalışmanın amacı eNOS varyantlarının (C894T ve 4VNTR b/a) Türk hastalarda Kronik lenfositik lösemi (KLL) riskini etkileyip etkilemediğini saptamaktır. Bu çalışmaya 60 KLL hastası ve benzer yaş ve cinsiyette 100 sağlıklı kontrol dahil edilmiştir. İki eNOS gen varyantı (G894T ve intron 4VNTR a/b) polimeraz zincir reaksiyonu (PZR) ve/veya restriksiyon fragman uzunluk polimorfizmi (RFLP) yöntemleri ile analiz edildi. Bu çalışmada, eNOS G894T varyantı TT genotipinin KLL hastalarında kontrole göre belirgin şekilde artmış risk ile ilişkili olduğunu bulduk (OR: 0.867, Cl: 0.785-0.957, p= 0.001). eNOS G894T allel dağılımında gruplar arasında belirgin fark yoktu (p> 0.05). Ayrıca eNOS intron 4'deki VNTR a/b varyant genotip ve allel sıklığı açısından KLL hastaları ve kontroller arasında belirgin fark saptanmadı. Bu çalışmada KLL hastalarındaki eNOS gen varyantları (G894T ve intron 4VNTR a/b) ilk defa eş zamanlı olarak incelenmiştir. Sonuçlarımız eNOS G894T varyantının Türk toplumunda KLL gelişmesi ile ilişkili olabileceğini önermektedir
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The Endothelial Nitric Oxide Synthase Gene Variants as a Risk Factor for Chronic Lymphocytic Leukemia

Öz:
Nitric oxide (NO) plays complicated roles in carcinogenesis. Endothelial nitric oxide synthase (eNOS) gene is responsible for most of the NO produced. For this reason, it was considered that the eNOS gene variants is associated with cancer suspectibility. The aim of this study was to determine whether eNOS variants (G894T and intron 4 VNTR a/b) affect in Chronic Lymphocytic Leukemia (CLL) risk in Turkish patients. This is a prospective single-center crosssectional study in patients with CLL. A total of 60 CLL patients and 100 healthy controls with similar age and sex were included to this study. Two eNOS gene variants (G894T and intron 4VNTR a/b) were analysed with polymerase chain reaction (PCR) and/or restriction fragment length polymorphism (RFLP) methods. In this study, we found that the TT genotype of eNOS G894T variant was significantly associated with an increased risk in patient with CLL compared with control (OR: 0.867, Cl: 0.785-0.957, p= 0.001). There was not any significant difference in the eNOS G894T allele distribution between the groups (p> 0.05). In addition, no significant difference was detected between the CLL patients and healthy controls with respect to the frequencies of genotypes and alleles in intron 4 VNTR a/b variant of eNOS. eNOS gene variants (G894T and intron 4 VNTR a/b) in CLL patients were simultaneously analyzed for the first time in present study. Our study suggest that the eNOS G894T variant may be associated with the development of CLL in the Turkish population
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APA PEHLİVAN M, PEHLİVAN S, SAHIN H, TOMATIR A, NURSAL A (2017). Kronik Lenfositik Lösemide Risk Faktörü Olarak Endotelyal Nitrik Oksit Sentaz Gen Varyantlari. Uluslararası Hematoloji-Onkoloji Dergisi, 27(2), 85 - 90.
Chicago PEHLİVAN MUSTAFA,PEHLİVAN SACİDE,SAHIN Handan H.,TOMATIR Ayse G,NURSAL Ayşe F Kronik Lenfositik Lösemide Risk Faktörü Olarak Endotelyal Nitrik Oksit Sentaz Gen Varyantlari. Uluslararası Hematoloji-Onkoloji Dergisi 27, no.2 (2017): 85 - 90.
MLA PEHLİVAN MUSTAFA,PEHLİVAN SACİDE,SAHIN Handan H.,TOMATIR Ayse G,NURSAL Ayşe F Kronik Lenfositik Lösemide Risk Faktörü Olarak Endotelyal Nitrik Oksit Sentaz Gen Varyantlari. Uluslararası Hematoloji-Onkoloji Dergisi, vol.27, no.2, 2017, ss.85 - 90.
AMA PEHLİVAN M,PEHLİVAN S,SAHIN H,TOMATIR A,NURSAL A Kronik Lenfositik Lösemide Risk Faktörü Olarak Endotelyal Nitrik Oksit Sentaz Gen Varyantlari. Uluslararası Hematoloji-Onkoloji Dergisi. 2017; 27(2): 85 - 90.
Vancouver PEHLİVAN M,PEHLİVAN S,SAHIN H,TOMATIR A,NURSAL A Kronik Lenfositik Lösemide Risk Faktörü Olarak Endotelyal Nitrik Oksit Sentaz Gen Varyantlari. Uluslararası Hematoloji-Onkoloji Dergisi. 2017; 27(2): 85 - 90.
IEEE PEHLİVAN M,PEHLİVAN S,SAHIN H,TOMATIR A,NURSAL A "Kronik Lenfositik Lösemide Risk Faktörü Olarak Endotelyal Nitrik Oksit Sentaz Gen Varyantlari." Uluslararası Hematoloji-Onkoloji Dergisi, 27, ss.85 - 90, 2017.