Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance
Yıl: 2018 Cilt: 46 Sayı: 2 Sayfa Aralığı: 247 - 261 Metin Dili: İngilizce DOI: 10.15671/HJBC.2018.233 İndeks Tarihi: 12-11-2018
Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance
Öz: Enlightening effects of biocontrol agents as Trichoderma spp. to provide disease control by combating pathogens is an important alternative in agricultural crop production. To that end, 9 species of Hypocrea/ Trichoderma having green ascospores isolated from the soil that tea plants cultivated in are identified by ITS sequences and were found to be close relative of Trichoderma sect. Pachybasium (77%). Ethyl acetate extracts of fungal isolates exhibited the antimicrobial activity against to Vibrio sp, Serratia marcescens, Mycobacterium smegmatis and Bacillus cereus but no antifungal activity was detected. The highest level of inhibitory activity was observed against to M. smegmatis by Trichoderma harzianum ID4A, ID4B and ID6B. In dual culture test, all Trichoderma strains were found to be showing the highest inhibitory activity against to the plant pathogens Botrytis cinerea, Sclerotonia sclerotiorum and Rhizoctonia solani (AG3), but relatively low activity against to the entomopathogen fungi. Volatile metabolites of Trichoderma spp. caused maximum reduction in mycelial growth and sclerotial production. Tested strains showed the highest tolerance to the fungicide Dikozin, whereas the lowest tolerance was against to the Captan regardless of the dosage.
Anahtar Kelime: Konular:
Potansiyel Biyokontrol Ajanları Olarak Yerel Trichoderma spp. Türlerinin Karakterizasyonu, in vitro Antagonistik Aktivitelerinin ve Fungisit Toleransların İncelenmesi
Öz: Trichoderma spp. gibi biyokontrol ajanlarının etkilerini incelemek tarımsal üretimde patojenlerle mücadele yoluyla hastalık kontrolünü sağlamada önemli bir alternatiftir. Bu amaçla, çay bitkilerinin topraklarından izole edilmiş yeşil askosporlara sahip 9 Hypocrea/ Trichoderma türü, ITS sekansları ile tanımlanmış ve türlerin % 77 oranında Trichoderma sect. Pachybasium ile yakın akraba olduğu belirlenmiştir. Fungal izolatların etil asetat ekstraktlarının, Vibrio sp., Serratia marcescens, Mycobacterium smegmatis ve Bacillus cereus’a karşı antimikrobiyal aktivite sergilediği tespit edilmesine karşın, izolatlarda antifungal aktivite gözlenmemiştir. En yüksek inhibisyon aktivitesinin, Trichoderma harzianum ID4A, ID4B ve ID6B tarafından M. smegmatis’e karşı oluşturulduğu ortaya çıkarılmıştır. Çift kültür testinde, tüm Trichoderma suşlarının Botrytis cinerea, Sclerotonia sclerotiorum ve Rhizoctonia solani (AG3) bitki patojenlerine karşı en yüksek inhibitör aktivite gösterdiği, fakat entomopatojen mantarlara karşı nispeten düşük etkide oldukları bulunmuştur. Trichoderma spp. uçucu metabolitleri, misel artışı ve sklerotik üretimde maksimum azalmaya neden olmuştur. Test edilen suşların, dozajdan bağımsız olarak en yüksek toleransı Dikozin fungisitine karşı, en düşük toleransı ise; Captan’a karşı gösterdiği belirlenmiştir.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Alpay Karaoğlu Ş, BOZDEVECİ A, PEHLİVAN GEDİK N (2018). Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance. , 247 - 261. 10.15671/HJBC.2018.233 |
| Chicago | Alpay Karaoğlu Şengül,BOZDEVECİ ARİF,PEHLİVAN GEDİK NECLA Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance. (2018): 247 - 261. 10.15671/HJBC.2018.233 |
| MLA | Alpay Karaoğlu Şengül,BOZDEVECİ ARİF,PEHLİVAN GEDİK NECLA Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance. , 2018, ss.247 - 261. 10.15671/HJBC.2018.233 |
| AMA | Alpay Karaoğlu Ş,BOZDEVECİ A,PEHLİVAN GEDİK N Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance. . 2018; 247 - 261. 10.15671/HJBC.2018.233 |
| Vancouver | Alpay Karaoğlu Ş,BOZDEVECİ A,PEHLİVAN GEDİK N Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance. . 2018; 247 - 261. 10.15671/HJBC.2018.233 |
| IEEE | Alpay Karaoğlu Ş,BOZDEVECİ A,PEHLİVAN GEDİK N "Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance." , ss.247 - 261, 2018. 10.15671/HJBC.2018.233 |
| ISNAD | Alpay Karaoğlu, Şengül vd. "Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance". (2018), 247-261. https://doi.org/10.15671/HJBC.2018.233 |
| APA | Alpay Karaoğlu Ş, BOZDEVECİ A, PEHLİVAN GEDİK N (2018). Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance. Hacettepe Journal of Biology and Chemistry, 46(2), 247 - 261. 10.15671/HJBC.2018.233 |
| Chicago | Alpay Karaoğlu Şengül,BOZDEVECİ ARİF,PEHLİVAN GEDİK NECLA Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance. Hacettepe Journal of Biology and Chemistry 46, no.2 (2018): 247 - 261. 10.15671/HJBC.2018.233 |
| MLA | Alpay Karaoğlu Şengül,BOZDEVECİ ARİF,PEHLİVAN GEDİK NECLA Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance. Hacettepe Journal of Biology and Chemistry, vol.46, no.2, 2018, ss.247 - 261. 10.15671/HJBC.2018.233 |
| AMA | Alpay Karaoğlu Ş,BOZDEVECİ A,PEHLİVAN GEDİK N Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance. Hacettepe Journal of Biology and Chemistry. 2018; 46(2): 247 - 261. 10.15671/HJBC.2018.233 |
| Vancouver | Alpay Karaoğlu Ş,BOZDEVECİ A,PEHLİVAN GEDİK N Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance. Hacettepe Journal of Biology and Chemistry. 2018; 46(2): 247 - 261. 10.15671/HJBC.2018.233 |
| IEEE | Alpay Karaoğlu Ş,BOZDEVECİ A,PEHLİVAN GEDİK N "Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance." Hacettepe Journal of Biology and Chemistry, 46, ss.247 - 261, 2018. 10.15671/HJBC.2018.233 |
| ISNAD | Alpay Karaoğlu, Şengül vd. "Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance". Hacettepe Journal of Biology and Chemistry 46/2 (2018), 247-261. https://doi.org/10.15671/HJBC.2018.233 |
