Aim: This study aims to determine the primary infertility-related stress levels of infertile women and some of the affecting factors. Design: This descriptive study was conducted at a private IVF center in a province in the Mediterranean region of Turkey, and the study data were collected between January and April 2016. The study population consisted of primary infertile women, who were treated at the relevant center between the dates mentioned. The sample of the study consisted of 577 primary infertile women who met the inclusion criteria and agreed to participate in the research. The Personal Information Form and the Infertility Stress Scale were used to collect data. Results: It was determined that the average age of the women included in the study was 28.35±4.11, 44.5% was university graduate and the infertility cause of 28.6% was her spouse. It was found that the mean score of the women in the stress in personal area sub-scale was 17.59±1.97, the mean score in the stress in marriage area was 10.52±2.13, and the mean score in the stress in social area sub-scale was 7.18±2.76. The difference between the mean sub-scale scores of the Infertility Stress Scale was found to be significant in terms of women's education level, professions, income levels, place of residence, infertility cause, infertility and marriage duration, treatment duration, type of previous treatment and the way of covering the treatment costs (p<0.05). Conclusion: It was determined that infertile women experience moderate stress in the social area, whereas they experience high level of stress in personal and marital area. It was also found that the women's education level, profession, income level, place of residence, infertility cause, infertility and marriage duration, treatment duration, type of previous treatment and the way of covering the treatment costs affect their stress levels.