The purpose of this study is to determine the institutionalization perception of hotel employees and reveal whether their institutionalization perception levels change or not according to different variables. The data of the research was obtained by surveying hotel employees working at the hotels with tourism operation license in six cities which hosts the most tourists and have the most tourism operation licenses in Turkey. According to the results of the study, there is significant differences among the institutionalization perception levels of employees with regard to the variables which are businesses’ having multiple branches, their belonging to an international brand and proprietorship of the top management. The outcomes of higher institutionalization perception levels of hotel employees who work at the hotels which have multiple branches, belong to an international brand and the top management of which are not composed of business owners but of professional managers are significant results of the study. Along with these results, it is thought that the study is going to provide a different point of view for the hotel business managers who plan to start the process of institutionalization and contribute to future researches of institutionalization in tourism businesses..