Objective: This cross section study has been conducted to determine the burnout level of the nurses working in a university hospital and to analyze the relationship between burnout and socio-demographic variables. Methods: The study has been done on 192 nurses who agreed to participate. Data has been collected by a sociodemographic data form consisting of 31 questions and consisting of 22 questions Maslach Burnout Inventory (MBI). Results: The nurses’ MBI emotional exhaustion average subscale points were determined as 20.3±7.0, depersonalization subscale average points were determined as 6.0±3.2 and personal accomplishment average subscale points were determined as 19.3±4.1. Gender, perceived income level, presence of chronic disease, history of receiving psychiatric help, total length of service in clinic, problem life while taking annual leave, occupational compliance, adherence to the profession, love of the profession and the possibility of professional development of the institution were found to affect burnout. (p<0.05). Conclusions: The level of emotional exhaustion of nurses is determined to be high, their depersonalization level is medium and personal accomplishment level is in inverse proportion and low. Burnout syndrome can be experienced as a result of the interaction between the personal qualities, workplace and professional qualities of the nurses. All these variables affect the burnout level. The burnout of nurses must be evaluated in both personal and organizational perspective.