Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender

The aim of the current study was to determine the optimum concentration of bovine serum albumin for post-thawing quality of drone sperm and this is the first study to evaluate the effect of BSA supplemented TL-Hepes based extenders for drone semen cryopreservation. Sexually mature drones were used for semen collection. Pooled semen was diluted with TL-Hepes based extender supplemented with different concentrations of BSA (1 mg/mL, 3 mg/mL, and 5 mg/mL) or without BSA (control), at a final concentration of 100x106 spermatozoon/mL. Motility, plasma membrane functional integrity (HOST), and defected acrosome (PSA-FITC) were evaluated in the study. At post thaw, the highest sperm motility rates were obtained in the BSA5 group (P<0.05). Functional integrity of sperm membrane was better preserved in the BSA3 and BSA5 groups compared to the other groups.The acrosomal integrity rates were higher in BSA5 group than in control group (P<0.05). The study shows that bovine serum albumin supplemented TL-Hepes based extenders have beneficial effect on drone semen parameters at post-thaw. The results of the study demonstrated a notable advantage of using 5 mg/mL of BSA in TL-Hepes based extender.

Anahtar Kelime:

Arı Spermasının Protein Eklenmiş TL-Hepes Bazlı Sulandırıcı İle Dondurulması

Öz:

Bu çalışmanın amacı, arı spermasının çözdürme sonrası kalitesi için en uygun sığır serum albümin konsantrasyonunu belirlemekti ve bu çalışma, arı spermasının dondurulması için BSA eklenmiş TL-Hepes bazlı sulandırıcıların etkisini değerlendiren ilk çalışmadır. Cinsel olarak olgun arılar sperma toplanması için kullanıldı. Alınan spermalar bir araya getirildikten sonra final konsantrasyonu 100x106 spermatozoon/ mL olacak şekilde farklı dozlarda BSA içeren (1 mg/mL, 3 mg/mL, ve 5 mg/mL) ve içermeyen (kontrol) TL-Hepes bazlı sulandırıcılarla sulandırılmıştır. Spermanın değerlendirilmesi amacıyla motilite, plazma membran fonksiyonel bütünlüğü (HOST), ve akrozomal bozukluk (PSA-FITC) değerlerine bakılmıştır. Eritme sonrası en yüksek motilite oranları BSA5 grubunda elde edilmiştir (P<0.05). BSA3 ve BSA5 gruplarında fonksiyonel membran bütünlüğü diğer gruplara göre daha iyi korunmuştur. Akrozomal bütünlük oranları BSA5 grubunda kontrol grubuna göre daha yüksek bulunmuştur (P<0.05). Çalışma sonucunda, sığır serum albümini eklenmiş TL-Hepes bazlı sulandırıcıların eritme sonrası arı sperm parametreleri üzerinde yararlı etkisi olduğu görülmektedir. Çalışma sonucu elde edilen veriler göz önüne alındığında TL-Hepes bazlı sulandırıcıya 5 mg/mL BSA ilavesinin önemli bir avantaj oluşturduğu görülmektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Alçay S, ÇAKMAK S, ÇAKMAK İ, Mülkpınar E, TOKER M, ÜSTÜNER B, ŞEN H, NUR Z (2019). Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. , 554 - 557. DOI: 10.9775/kvfd.2018.21311
Chicago	Alçay Selim,ÇAKMAK Selvinar SEVEN,ÇAKMAK İbrahim,Mülkpınar Emine,TOKER Mehmed Berk,ÜSTÜNER Burcu,ŞEN Hasan,NUR ZEKARIYA Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. (2019): 554 - 557. DOI: 10.9775/kvfd.2018.21311
MLA	Alçay Selim,ÇAKMAK Selvinar SEVEN,ÇAKMAK İbrahim,Mülkpınar Emine,TOKER Mehmed Berk,ÜSTÜNER Burcu,ŞEN Hasan,NUR ZEKARIYA Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. , 2019, ss.554 - 557. DOI: 10.9775/kvfd.2018.21311
AMA	Alçay S,ÇAKMAK S,ÇAKMAK İ,Mülkpınar E,TOKER M,ÜSTÜNER B,ŞEN H,NUR Z Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. . 2019; 554 - 557. DOI: 10.9775/kvfd.2018.21311
Vancouver	Alçay S,ÇAKMAK S,ÇAKMAK İ,Mülkpınar E,TOKER M,ÜSTÜNER B,ŞEN H,NUR Z Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. . 2019; 554 - 557. DOI: 10.9775/kvfd.2018.21311
IEEE	Alçay S,ÇAKMAK S,ÇAKMAK İ,Mülkpınar E,TOKER M,ÜSTÜNER B,ŞEN H,NUR Z "Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender." , ss.554 - 557, 2019. DOI: 10.9775/kvfd.2018.21311
ISNAD	Alçay, Selim vd. "Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender". (2019), 554-557. https://doi.org/DOI: 10.9775/kvfd.2018.21311

APA	Alçay S, ÇAKMAK S, ÇAKMAK İ, Mülkpınar E, TOKER M, ÜSTÜNER B, ŞEN H, NUR Z (2019). Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25(4), 554 - 557. DOI: 10.9775/kvfd.2018.21311
Chicago	Alçay Selim,ÇAKMAK Selvinar SEVEN,ÇAKMAK İbrahim,Mülkpınar Emine,TOKER Mehmed Berk,ÜSTÜNER Burcu,ŞEN Hasan,NUR ZEKARIYA Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 25, no.4 (2019): 554 - 557. DOI: 10.9775/kvfd.2018.21311
MLA	Alçay Selim,ÇAKMAK Selvinar SEVEN,ÇAKMAK İbrahim,Mülkpınar Emine,TOKER Mehmed Berk,ÜSTÜNER Burcu,ŞEN Hasan,NUR ZEKARIYA Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.25, no.4, 2019, ss.554 - 557. DOI: 10.9775/kvfd.2018.21311
AMA	Alçay S,ÇAKMAK S,ÇAKMAK İ,Mülkpınar E,TOKER M,ÜSTÜNER B,ŞEN H,NUR Z Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2019; 25(4): 554 - 557. DOI: 10.9775/kvfd.2018.21311
Vancouver	Alçay S,ÇAKMAK S,ÇAKMAK İ,Mülkpınar E,TOKER M,ÜSTÜNER B,ŞEN H,NUR Z Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2019; 25(4): 554 - 557. DOI: 10.9775/kvfd.2018.21311
IEEE	Alçay S,ÇAKMAK S,ÇAKMAK İ,Mülkpınar E,TOKER M,ÜSTÜNER B,ŞEN H,NUR Z "Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25, ss.554 - 557, 2019. DOI: 10.9775/kvfd.2018.21311
ISNAD	Alçay, Selim vd. "Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 25/4 (2019), 554-557. https://doi.org/DOI: 10.9775/kvfd.2018.21311