Objective: The aim of this study was to determine the perceptions of patient safety culture of nurses working in a private hospital. Materials and Methods: The sample of the study consisted of 145 nurses with JCI standards and working in a private hospital with proactive levels of patient safety. After obtaining the permission of the ethics committee and the permission of the institution, the research data were collected with the “Personal Information Form” and the “Patient Safety Culture Scale” (PSCS) by face-to-face interview method. SPSS v.25 program was used to analyze the data. Descriptive statistics for continuous (quantitative) variables; Categorical variables are given as numbers and percentages while “mean, standard deviation, minimum and maximum” “Independent T-test and One-Way Variance Analysis (ANOVA)” were applied after examining scale sub-dimensions according to Normality distribution. Results: The mean total score of the nurses from the PSCS was 3.23 ± 0.37 points. In addition, all nurses' perception of security culture boyut was above average in all sub-dimensions of the scale. The highest score was determined by the “training of the employees” (3,35 ± 0,44), the lowest score by “unexpected event and error reporting” (3,13 ± 0,51). A significant difference was found between the duration of the study and the sub-dimensions of the study. In the study, the mean total and subscale scores of the PSCS scale of 11 years and over employees were found to be statistically significantly higher than those who worked between 1-5 years and 6-10 years. Conclusions: The results of these study findings indicate that nurses have worked in the institution for a long time and that they have a it has contributed to the increase in perceptions of patient safety culture in parallel with the years of study. Patient safety has always been the most important function of nurses. Reporting errors while giving clinical care and discussing about the subject is the key of a good safety culture.