Due to the use of intensive chemical fertilizers and pesticides in agriculture are increasing risks related to human and animal health and environmental problems. Therefore, interest in alternative methods is increasing day by day. Therefore, the demand in alternative methods especially in plant nutrition and plant protection products is increasing day by day. The use of organic and biological solutions in agriculture is one of these alternative methods. The importance of the use of beneficial bacteria in agriculture, which are one of the biological solutions, is increasingly understood. In this study, Bacillus subtilis TV-17C bacterial isolate, which fixes nitrogen, dissolves phosphate, dissolves calcium, produces amino acids and organic acids and produces hormones, was formulated with an organic liquid carrier and used in apple sapling cultivation. The effect of this bacterial application on some plant growth parameters, hormone levels in the plant, macro and micro nutrient levels in the plant and soil and some soil properties were investigated in this study. As a result of the research; this bacterial microbial fertilizer formulation has been shown to cause increases in all plant growth parameters and hormone levels in plants, macro and micro nutrients levels in plants and soil. Considering all these versatile effects of B. subtilis TV-17C bacterial strain, it is planned to make the necessary applications for the registration of this formulation under the trade name Stoma Fix.